TY - JOUR T1 - Laser-induced ocular hypertension in adult rats does not affect non-RGC neurons in the ganglion cell layer but results in protracted severe loss of cone-photoreceptors. AU - Ortín-Martínez,Arturo, AU - Salinas-Navarro,Manuel, AU - Nadal-Nicolás,Francisco Manuel, AU - Jiménez-López,Manuel, AU - Valiente-Soriano,Francisco Javier, AU - García-Ayuso,Diego, AU - Bernal-Garro,José Manuel, AU - Avilés-Trigueros,Marcelino, AU - Agudo-Barriuso,Marta, AU - Villegas-Pérez,María Paz, AU - Vidal-Sanz,Manuel, Y1 - 2015/01/07/ PY - 2014/08/21/received PY - 2014/11/19/revised PY - 2015/01/07/accepted PY - 2015/1/11/entrez PY - 2015/1/13/pubmed PY - 2015/5/6/medline KW - Adult albino rat retinal ganglion cells KW - Displaced amacrine cells KW - Elevated intraocular pressure KW - Ganglion cell layer of the retina KW - Limbar laser photocoagulation KW - Neuronal degeneration KW - Ocular hypertension KW - Outer retinal layers KW - Photoreceptors loss KW - Rodent experimental glaucoma model KW - S-cones L-cones SP - 17 EP - 33 JF - Experimental eye research JO - Exp Eye Res VL - 132 N2 - To investigate the long-term effects of laser-photocoagulation (LP)-induced ocular hypertension (OHT) in the innermost and outermost (outer-nuclear and outer segment)-retinal layers (ORL). OHT was induced in the left eye of adult rats. To investigate the ganglion cell layer (GCL) wholemounts were examined at 1, 3 or 6 months using Brn3a-immunodetection to identify retinal ganglion cells (RGCs) and DAPI-staining to detect all nuclei in this layer. To study the effects of LP on the ORL up to 6 months, retinas were: i) fresh extracted to quantify the levels of rod-, S- and L-opsin; ii) cut in cross-sections for morphometric analysis, or; iii) prepared as wholemounts to quantify and study retinal distributions of entire populations of RGCs (retrogradely labeled with fluorogold, FG), S- and L-cones (immunolabeled). OHT resulted in wedge-like sectors with their apex on the optic disc devoid of Brn3a(+)RGCs but with large numbers of DAPI(+)nuclei. The levels of all opsins diminished by 2 weeks and further decreased to 20% of basal-levels by 3 months. Cross-sections revealed focal areas of ORL degeneration. RGC survival at 15 days represented approximately 28% and did not change with time, whereas the S- and L-cone populations diminished to 65% and 80%, or to 20 and 35% at 1 or 6 months, respectively. In conclusion, LP induces in the GCL selective RGCs loss that does not progress after 1 month, and S- and L-cone loss that progresses for up to 6 months. Thus, OHT results in severe damage to both the innermost and the ORL. SN - 1096-0007 UR - https://www.unboundmedicine.com/medline/citation/25576772/Laser_induced_ocular_hypertension_in_adult_rats_does_not_affect_non_RGC_neurons_in_the_ganglion_cell_layer_but_results_in_protracted_severe_loss_of_cone_photoreceptors_ DB - PRIME DP - Unbound Medicine ER -