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Development of nanofibrous scaffolds containing gum tragacanth/poly (ε-caprolactone) for application as skin scaffolds.
Mater Sci Eng C Mater Biol Appl. 2015 Mar; 48:71-9.MS

Abstract

Outstanding wound healing activity of gum tragacanth (GT) and higher mechanical strength of poly (ε-caprolactone) (PCL) may produce an excellent nanofibrous patch for either skin tissue engineering or wound dressing application. PCL/GT scaffold containing different concentrations of PCL with different blend ratios of GT/PCL was produced using 90% acetic acid as solvent. The results demonstrated that the PCL/GT (3:1.5) with PCL concentration of 20% (w/v) produced nanofibers with proper morphology. Scanning electron microscopy (SEM) and differential scanning calorimetry (DSC) were utilized to characterize the nanofibers. Surface wettability, functional groups analysis, porosity and tensile properties of nanofibers were evaluated. Morphological characterization showed that the addition of GT to PCL solution results in decreasing the average diameter of the PCL/GT nanofibers. However, the hydrophilicity increased in the PCL/GT nanofibers. Slight increase in melting peaks was observed due to the blending of PCL with GT nanofibers. PCL/GT nanofibers were used for in vitro cell culture of human fibroblast cell lines AGO and NIH 3T3 fibroblast cells. MTT assay and SEM results showed that the biocomposite PCL/GT mats enhanced the fibroblast adhesion and proliferation compared to PCL scaffolds. The antibacterial activity of PCL/GT and GT nanofibers against Staphylococcus aureus and Pseudomonas aeruginosa was also examined.

Authors+Show Affiliations

Textile Engineering Department, Amirkabir University of Technology, Tehran, Iran.Textile Engineering Department, Amirkabir University of Technology, Tehran, Iran; Center for excellence Modern Textile Characterization, Tehran, Iran. Electronic address: hajirb@aut.ac.ir.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25579898

Citation

Ranjbar-Mohammadi, Marziyeh, and S Hajir Bahrami. "Development of Nanofibrous Scaffolds Containing Gum Tragacanth/poly (ε-caprolactone) for Application as Skin Scaffolds." Materials Science & Engineering. C, Materials for Biological Applications, vol. 48, 2015, pp. 71-9.
Ranjbar-Mohammadi M, Bahrami SH. Development of nanofibrous scaffolds containing gum tragacanth/poly (ε-caprolactone) for application as skin scaffolds. Mater Sci Eng C Mater Biol Appl. 2015;48:71-9.
Ranjbar-Mohammadi, M., & Bahrami, S. H. (2015). Development of nanofibrous scaffolds containing gum tragacanth/poly (ε-caprolactone) for application as skin scaffolds. Materials Science & Engineering. C, Materials for Biological Applications, 48, 71-9. https://doi.org/10.1016/j.msec.2014.10.020
Ranjbar-Mohammadi M, Bahrami SH. Development of Nanofibrous Scaffolds Containing Gum Tragacanth/poly (ε-caprolactone) for Application as Skin Scaffolds. Mater Sci Eng C Mater Biol Appl. 2015;48:71-9. PubMed PMID: 25579898.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of nanofibrous scaffolds containing gum tragacanth/poly (ε-caprolactone) for application as skin scaffolds. AU - Ranjbar-Mohammadi,Marziyeh, AU - Bahrami,S Hajir, Y1 - 2014/10/13/ PY - 2014/05/14/received PY - 2014/08/22/revised PY - 2014/10/08/accepted PY - 2015/1/13/entrez PY - 2015/1/13/pubmed PY - 2015/9/22/medline KW - Cell culture KW - Electrospinning KW - Gum tragacanth KW - Nanofiber KW - Poly (ε-caprolactone) SP - 71 EP - 9 JF - Materials science & engineering. C, Materials for biological applications JO - Mater Sci Eng C Mater Biol Appl VL - 48 N2 - Outstanding wound healing activity of gum tragacanth (GT) and higher mechanical strength of poly (ε-caprolactone) (PCL) may produce an excellent nanofibrous patch for either skin tissue engineering or wound dressing application. PCL/GT scaffold containing different concentrations of PCL with different blend ratios of GT/PCL was produced using 90% acetic acid as solvent. The results demonstrated that the PCL/GT (3:1.5) with PCL concentration of 20% (w/v) produced nanofibers with proper morphology. Scanning electron microscopy (SEM) and differential scanning calorimetry (DSC) were utilized to characterize the nanofibers. Surface wettability, functional groups analysis, porosity and tensile properties of nanofibers were evaluated. Morphological characterization showed that the addition of GT to PCL solution results in decreasing the average diameter of the PCL/GT nanofibers. However, the hydrophilicity increased in the PCL/GT nanofibers. Slight increase in melting peaks was observed due to the blending of PCL with GT nanofibers. PCL/GT nanofibers were used for in vitro cell culture of human fibroblast cell lines AGO and NIH 3T3 fibroblast cells. MTT assay and SEM results showed that the biocomposite PCL/GT mats enhanced the fibroblast adhesion and proliferation compared to PCL scaffolds. The antibacterial activity of PCL/GT and GT nanofibers against Staphylococcus aureus and Pseudomonas aeruginosa was also examined. SN - 1873-0191 UR - https://www.unboundmedicine.com/medline/citation/25579898/Development_of_nanofibrous_scaffolds_containing_gum_tragacanth/poly__ε_caprolactone__for_application_as_skin_scaffolds_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0928-4931(14)00640-7 DB - PRIME DP - Unbound Medicine ER -