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Antioxidant effects of proanthocyanidin-rich natural extracts from grape seed and cupuassu on gastrointestinal mucosa.
J Sci Food Agric. 2016 Jan 15; 96(1):178-82.JS

Abstract

BACKGROUND

The gastrointestinal tract (GI) is constantly exposed to reactive species released by the GI tract itself, and those present in food and beverages. Phenolic compounds may help in protecting the GI tract against damage produced by the reactive species. In this paper we have analyzed the effects of a grape seed proanthocyanidin extract (GSPE) on reactive oxygen species (ROS) production in two different intestinal cell types: the absorptive cell line Caco-2 and the enteroendocrine cell line STC-1.

RESULTS

We show that GSPE prevents tert-butylhydroperoxide-induced oxidative stress in both cell lines, and that the effects are dose and time dependent. We have also analyzed whether GSPE has any in vivo effect, and found that 25 mg kg(-1) body weight cannot counteract the increase in intestinal ROS induced by the cafeteria diet. However, an acute (1 h) treatment of 1 g GSPE kg(-1) body weight reduced ROS in fasted animals and also decreased ROS induction by food. These effects were found only after a short-term treatment. Furthermore, we have compared the in vitro GSPE effects with those of another proanthocyanidin-rich extract from cupuassu seeds, though it has compounds with different structures. Cupuassu extract also shows antioxidant effects in both cell types, which suggests different mechanisms from those of GSPE.

CONCLUSION

Natural proanthocyanidin-rich extracts have an antioxidant effect in the GI tract, acting on absorptive cells and enterohormone-secreting cells, although the effects depend on the dose and period of treatment. © 2015 Society of Chemical Industry.

Authors+Show Affiliations

MoBioFood Research Group, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, 43007 Tarragona, Spain.MoBioFood Research Group, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, 43007 Tarragona, Spain.Departamento de Alimentos e Nutriçao Experimental, Faculdade de Ciências Farmacêuticas, Universidade de Sao Paulo, 05508-900 Sao Paulo, SP, Brazil.MoBioFood Research Group, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, 43007 Tarragona, Spain.MoBioFood Research Group, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, 43007 Tarragona, Spain.MoBioFood Research Group, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, 43007 Tarragona, Spain.MoBioFood Research Group, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, 43007 Tarragona, Spain.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25582348

Citation

Pinent, Montserrat, et al. "Antioxidant Effects of Proanthocyanidin-rich Natural Extracts From Grape Seed and Cupuassu On Gastrointestinal Mucosa." Journal of the Science of Food and Agriculture, vol. 96, no. 1, 2016, pp. 178-82.
Pinent M, Castell-Auví A, Genovese MI, et al. Antioxidant effects of proanthocyanidin-rich natural extracts from grape seed and cupuassu on gastrointestinal mucosa. J Sci Food Agric. 2016;96(1):178-82.
Pinent, M., Castell-Auví, A., Genovese, M. I., Serrano, J., Casanova, A., Blay, M., & Ardévol, A. (2016). Antioxidant effects of proanthocyanidin-rich natural extracts from grape seed and cupuassu on gastrointestinal mucosa. Journal of the Science of Food and Agriculture, 96(1), 178-82. https://doi.org/10.1002/jsfa.7079
Pinent M, et al. Antioxidant Effects of Proanthocyanidin-rich Natural Extracts From Grape Seed and Cupuassu On Gastrointestinal Mucosa. J Sci Food Agric. 2016 Jan 15;96(1):178-82. PubMed PMID: 25582348.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant effects of proanthocyanidin-rich natural extracts from grape seed and cupuassu on gastrointestinal mucosa. AU - Pinent,Montserrat, AU - Castell-Auví,Anna, AU - Genovese,Maria Inés, AU - Serrano,Joan, AU - Casanova,Angela, AU - Blay,Mayte, AU - Ardévol,Anna, Y1 - 2015/02/10/ PY - 2014/09/26/received PY - 2014/12/24/revised PY - 2015/01/06/accepted PY - 2015/1/14/entrez PY - 2015/1/15/pubmed PY - 2016/10/8/medline KW - Enteroendocrine KW - ROS KW - cupuassu KW - enterocyte KW - grape KW - proanthocyanidin SP - 178 EP - 82 JF - Journal of the science of food and agriculture JO - J Sci Food Agric VL - 96 IS - 1 N2 - BACKGROUND: The gastrointestinal tract (GI) is constantly exposed to reactive species released by the GI tract itself, and those present in food and beverages. Phenolic compounds may help in protecting the GI tract against damage produced by the reactive species. In this paper we have analyzed the effects of a grape seed proanthocyanidin extract (GSPE) on reactive oxygen species (ROS) production in two different intestinal cell types: the absorptive cell line Caco-2 and the enteroendocrine cell line STC-1. RESULTS: We show that GSPE prevents tert-butylhydroperoxide-induced oxidative stress in both cell lines, and that the effects are dose and time dependent. We have also analyzed whether GSPE has any in vivo effect, and found that 25 mg kg(-1) body weight cannot counteract the increase in intestinal ROS induced by the cafeteria diet. However, an acute (1 h) treatment of 1 g GSPE kg(-1) body weight reduced ROS in fasted animals and also decreased ROS induction by food. These effects were found only after a short-term treatment. Furthermore, we have compared the in vitro GSPE effects with those of another proanthocyanidin-rich extract from cupuassu seeds, though it has compounds with different structures. Cupuassu extract also shows antioxidant effects in both cell types, which suggests different mechanisms from those of GSPE. CONCLUSION: Natural proanthocyanidin-rich extracts have an antioxidant effect in the GI tract, acting on absorptive cells and enterohormone-secreting cells, although the effects depend on the dose and period of treatment. © 2015 Society of Chemical Industry. SN - 1097-0010 UR - https://www.unboundmedicine.com/medline/citation/25582348/Antioxidant_effects_of_proanthocyanidin_rich_natural_extracts_from_grape_seed_and_cupuassu_on_gastrointestinal_mucosa_ L2 - https://doi.org/10.1002/jsfa.7079 DB - PRIME DP - Unbound Medicine ER -