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Silencing the expression of the salivary sheath protein causes transgenerational feeding suppression in the aphid Sitobion avenae.
Plant Biotechnol J. 2015 Aug; 13(6):849-57.PB

Abstract

Aphids produce gel saliva during feeding which forms a sheath around the stylet as it penetrates through the apoplast. The sheath is required for the sustained ingestion of phloem sap from sieve elements and is thought to form when the structural sheath protein (SHP) is cross-linked by intermolecular disulphide bridges. We investigated the possibility of controlling aphid infestation by host-induced gene silencing (HIGS) targeting shp expression in the grain aphid Sitobion avenae. When aphids were fed on transgenic barley expressing shp double-stranded RNA (shp-dsRNA), they produced significantly lower levels of shp mRNA compared to aphids feeding on wild-type plants, suggesting that the transfer of inhibitory RNA from the plant to the insect was successful. shp expression remained low when aphids were transferred from transgenic plants and fed for 1 or 2 weeks, respectively, on wild-type plants, confirming that silencing had a prolonged impact. Reduced shp expression correlated with a decline in growth, reproduction and survival rates. Remarkably, morphological and physiological aberrations such as winged adults and delayed maturation were maintained over seven aphid generations feeding on wild-type plants. Targeting shp expression therefore appears to cause strong transgenerational effects on feeding, development and survival in S. avenae, suggesting that the HIGS technology has a realistic potential for the control of aphid pests in agriculture.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Centre for BioSystems, Land Use and Nutrition, Institute of Phytopathology and Applied Zoology, Justus Liebig University, Giessen, Germany.

Centre for BioSystems, Land Use and Nutrition, Institute of Phytopathology and Applied Zoology, Justus Liebig University, Giessen, Germany.

Centre for BioSystems, Land Use and Nutrition, Institute of Phytopathology and Applied Zoology, Justus Liebig University, Giessen, Germany.

Centre for BioSystems, Land Use and Nutrition, Institute of Phytopathology and Applied Zoology, Justus Liebig University, Giessen, Germany.

Centre for BioSystems, Land Use and Nutrition, Institute of Phytopathology and Applied Zoology, Justus Liebig University, Giessen, Germany. Project Group 'Bioresources', Fraunhofer Institute of Molecular Biology and Applied Ecology IME, Giessen, Germany.

Centre for BioSystems, Land Use and Nutrition, Institute of Phytopathology and Applied Zoology, Justus Liebig University, Giessen, Germany.

MeSH

AnimalsAphidsFeeding BehaviorGene SilencingMolecular Sequence DataSalivary Proteins and Peptides

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25586210

Citation

Abdellatef, Eltayb, et al. "Silencing the Expression of the Salivary Sheath Protein Causes Transgenerational Feeding Suppression in the Aphid Sitobion Avenae." Plant Biotechnology Journal, vol. 13, no. 6, 2015, pp. 849-57.

Abdellatef E, Will T, Koch A, et al. Silencing the expression of the salivary sheath protein causes transgenerational feeding suppression in the aphid Sitobion avenae. Plant Biotechnol J. 2015;13(6):849-57.

Abdellatef, E., Will, T., Koch, A., Imani, J., Vilcinskas, A., & Kogel, K. H. (2015). Silencing the expression of the salivary sheath protein causes transgenerational feeding suppression in the aphid Sitobion avenae. Plant Biotechnology Journal, 13(6), 849-57. https://doi.org/10.1111/pbi.12322

Abdellatef E, et al. Silencing the Expression of the Salivary Sheath Protein Causes Transgenerational Feeding Suppression in the Aphid Sitobion Avenae. Plant Biotechnol J. 2015;13(6):849-57. PubMed PMID: 25586210.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Silencing the expression of the salivary sheath protein causes transgenerational feeding suppression in the aphid Sitobion avenae. AU - Abdellatef,Eltayb, AU - Will,Torsten, AU - Koch,Aline, AU - Imani,Jafargholi, AU - Vilcinskas,Andreas, AU - Kogel,Karl-Heinz, Y1 - 2015/01/14/ PY - 2014/08/29/received PY - 2014/11/07/revised PY - 2014/12/04/accepted PY - 2015/1/15/entrez PY - 2015/1/15/pubmed PY - 2016/4/6/medline KW - RNAi KW - Sitobion avenae KW - aphid KW - host-induced gene silencing KW - salivary sheath protein KW - transgenerational silencing SP - 849 EP - 57 JF - Plant biotechnology journal JO - Plant Biotechnol J VL - 13 IS - 6 N2 - Aphids produce gel saliva during feeding which forms a sheath around the stylet as it penetrates through the apoplast. The sheath is required for the sustained ingestion of phloem sap from sieve elements and is thought to form when the structural sheath protein (SHP) is cross-linked by intermolecular disulphide bridges. We investigated the possibility of controlling aphid infestation by host-induced gene silencing (HIGS) targeting shp expression in the grain aphid Sitobion avenae. When aphids were fed on transgenic barley expressing shp double-stranded RNA (shp-dsRNA), they produced significantly lower levels of shp mRNA compared to aphids feeding on wild-type plants, suggesting that the transfer of inhibitory RNA from the plant to the insect was successful. shp expression remained low when aphids were transferred from transgenic plants and fed for 1 or 2 weeks, respectively, on wild-type plants, confirming that silencing had a prolonged impact. Reduced shp expression correlated with a decline in growth, reproduction and survival rates. Remarkably, morphological and physiological aberrations such as winged adults and delayed maturation were maintained over seven aphid generations feeding on wild-type plants. Targeting shp expression therefore appears to cause strong transgenerational effects on feeding, development and survival in S. avenae, suggesting that the HIGS technology has a realistic potential for the control of aphid pests in agriculture. SN - 1467-7652 UR - https://www.unboundmedicine.com/medline/citation/25586210/Silencing_the_expression_of_the_salivary_sheath_protein_causes_transgenerational_feeding_suppression_in_the_aphid_Sitobion_avenae_ DB - PRIME DP - Unbound Medicine ER -