Tags

Type your tag names separated by a space and hit enter

TRPM8 function and expression in vagal sensory neurons and afferent nerves innervating guinea pig esophagus.
Am J Physiol Gastrointest Liver Physiol. 2015 Mar 15; 308(6):G489-96.AJ

Abstract

Sensory transduction in esophageal afferents requires specific ion channels and receptors. TRPM8 is a new member of the transient receptor potential (TRP) channel family and participates in cold- and menthol-induced sensory transduction, but its role in visceral sensory transduction is still less clear. This study aims to determine TRPM8 function and expression in esophageal vagal afferent subtypes. TRPM8 agonist WS-12-induced responses were first determined in nodose and jugular neurons by calcium imaging and then investigated by whole cell patch-clamp recordings in Dil-labeled esophageal nodose and jugular neurons. Extracellular single-unit recordings were performed in nodose and jugular C fiber neurons using ex vivo esophageal-vagal preparations with intact nerve endings in the esophagus. TRPM8 mRNA expression was determined by single neuron RT-PCR in Dil-labeled esophageal nodose and jugular neurons. The TRPM8 agonist WS-12 elicited calcium influx in a subpopulation of jugular but not nodose neurons. WS-12 activated outwardly rectifying currents in esophageal Dil-labeled jugular but not nodose neurons in a dose-dependent manner, which could be inhibited by the TRPM8 inhibitor AMTB. WS-12 selectively evoked action potential discharges in esophageal jugular but not nodose C fibers. Consistently, TRPM8 transcripts were highly expressed in esophageal Dil-labeled TRPV1-positive jugular neurons. In summary, the present study demonstrated a preferential expression and function of TRPM8 in esophageal vagal jugular but not nodose neurons and C fiber subtypes. This provides a distinctive role of TRPM8 in esophageal sensory transduction and may lead to a better understanding of the mechanisms of esophageal sensation and nociception.

Authors+Show Affiliations

Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland.Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland.Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland.Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland.Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland.Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland syyu@jhmi.edu.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25591866

Citation

Yu, Xiaoyun, et al. "TRPM8 Function and Expression in Vagal Sensory Neurons and Afferent Nerves Innervating Guinea Pig Esophagus." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 308, no. 6, 2015, pp. G489-96.
Yu X, Hu Y, Ru F, et al. TRPM8 function and expression in vagal sensory neurons and afferent nerves innervating guinea pig esophagus. Am J Physiol Gastrointest Liver Physiol. 2015;308(6):G489-96.
Yu, X., Hu, Y., Ru, F., Kollarik, M., Undem, B. J., & Yu, S. (2015). TRPM8 function and expression in vagal sensory neurons and afferent nerves innervating guinea pig esophagus. American Journal of Physiology. Gastrointestinal and Liver Physiology, 308(6), G489-96. https://doi.org/10.1152/ajpgi.00336.2014
Yu X, et al. TRPM8 Function and Expression in Vagal Sensory Neurons and Afferent Nerves Innervating Guinea Pig Esophagus. Am J Physiol Gastrointest Liver Physiol. 2015 Mar 15;308(6):G489-96. PubMed PMID: 25591866.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TRPM8 function and expression in vagal sensory neurons and afferent nerves innervating guinea pig esophagus. AU - Yu,Xiaoyun, AU - Hu,Youtian, AU - Ru,Fei, AU - Kollarik,Marian, AU - Undem,Bradley J, AU - Yu,Shaoyong, Y1 - 2015/01/15/ PY - 2015/1/17/entrez PY - 2015/1/17/pubmed PY - 2015/5/13/medline KW - C fiber KW - TRPM8 KW - esophagus KW - menthol KW - vagal SP - G489 EP - 96 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am J Physiol Gastrointest Liver Physiol VL - 308 IS - 6 N2 - Sensory transduction in esophageal afferents requires specific ion channels and receptors. TRPM8 is a new member of the transient receptor potential (TRP) channel family and participates in cold- and menthol-induced sensory transduction, but its role in visceral sensory transduction is still less clear. This study aims to determine TRPM8 function and expression in esophageal vagal afferent subtypes. TRPM8 agonist WS-12-induced responses were first determined in nodose and jugular neurons by calcium imaging and then investigated by whole cell patch-clamp recordings in Dil-labeled esophageal nodose and jugular neurons. Extracellular single-unit recordings were performed in nodose and jugular C fiber neurons using ex vivo esophageal-vagal preparations with intact nerve endings in the esophagus. TRPM8 mRNA expression was determined by single neuron RT-PCR in Dil-labeled esophageal nodose and jugular neurons. The TRPM8 agonist WS-12 elicited calcium influx in a subpopulation of jugular but not nodose neurons. WS-12 activated outwardly rectifying currents in esophageal Dil-labeled jugular but not nodose neurons in a dose-dependent manner, which could be inhibited by the TRPM8 inhibitor AMTB. WS-12 selectively evoked action potential discharges in esophageal jugular but not nodose C fibers. Consistently, TRPM8 transcripts were highly expressed in esophageal Dil-labeled TRPV1-positive jugular neurons. In summary, the present study demonstrated a preferential expression and function of TRPM8 in esophageal vagal jugular but not nodose neurons and C fiber subtypes. This provides a distinctive role of TRPM8 in esophageal sensory transduction and may lead to a better understanding of the mechanisms of esophageal sensation and nociception. SN - 1522-1547 UR - https://www.unboundmedicine.com/medline/citation/25591866/TRPM8_function_and_expression_in_vagal_sensory_neurons_and_afferent_nerves_innervating_guinea_pig_esophagus_ DB - PRIME DP - Unbound Medicine ER -