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Dissecting the function of the different chitin synthases in vegetative growth and sexual development in Neurospora crassa.
Fungal Genet Biol. 2015 Feb; 75:30-45.FG

Abstract

Chitin, one of the most important carbohydrates of the fungal cell wall, is synthesized by chitin synthases (CHS). Seven sequences encoding CHSs have been identified in the genome of Neurospora crassa. Previously, CHS-1, -3 and -6 were found at the Spitzenkörper(Spk) core and developing septa. We investigated the functional importance of each CHS in growth and development of N. crassa. The cellular distribution of each CHS tagged with fluorescent proteins and the impact of corresponding gene deletions on vegetative growth and sexual development were compared. CHS-2, -4, -5 and -7 were also found at the core of the Spk and in forming septa in vegetative hyphae. As the septum ring developed, CHS-2-GFP remained at the growing edge of the septum until it localized around the septal pore. In addition, all CHSs were located in cross-walls of conidiophores. A partial co-localization of CHS-1-m and CHS-5-GFP or CHS-2-GFP occurred in the Spk and septa. Analyses of deletion mutants suggested that CHS-6 has a role primarily in hyphal extension and ascospore formation, CHS-5 in aerial hyphae, conidia and ascospore formation, CHS-3 in perithecia development and CHS-7 in all of the aforementioned. We show that chs-7/csmB fulfills a sexual function and chs-6/chsG fulfills a vegetative growth function in N. crassa but not in Aspergillus nidulans, whereas vice versa chs-2/chsA fulfills a sexual function in A. nidulans but not in N. crassa. This suggests that different classes of CHSs can fulfill distinct developmental functions in various fungi. Immunoprecipitation followed by mass spectrometry of CHS-1-GFP, CHS-4-GFP and CHS-5-GFP identified distinct putative interacting proteins for each CHS. Collectively, our results suggest that there are distinct populations of chitosomes, each carrying specific CHSs, with particular roles during different developmental stages.

Authors+Show Affiliations

Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada, CICESE, Ctra. Ensenada-Tijuana No. 3918, Ensenada, Baja California C.P. 22860, Mexico.Department of Molecular Microbiology and Genetics, Georg-August Universität Göttingen, Grisebachstrasse 8, 37077 Göttingen, Germany.Department of Molecular Microbiology and Genetics, Georg-August Universität Göttingen, Grisebachstrasse 8, 37077 Göttingen, Germany.Department of Molecular Microbiology and Genetics, Georg-August Universität Göttingen, Grisebachstrasse 8, 37077 Göttingen, Germany.Department of Molecular Microbiology and Genetics, Georg-August Universität Göttingen, Grisebachstrasse 8, 37077 Göttingen, Germany.Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada, CICESE, Ctra. Ensenada-Tijuana No. 3918, Ensenada, Baja California C.P. 22860, Mexico. Electronic address: riquelme@cicese.mx.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25596036

Citation

Fajardo-Somera, Rosa A., et al. "Dissecting the Function of the Different Chitin Synthases in Vegetative Growth and Sexual Development in Neurospora Crassa." Fungal Genetics and Biology : FG & B, vol. 75, 2015, pp. 30-45.
Fajardo-Somera RA, Jöhnk B, Bayram Ö, et al. Dissecting the function of the different chitin synthases in vegetative growth and sexual development in Neurospora crassa. Fungal Genet Biol. 2015;75:30-45.
Fajardo-Somera, R. A., Jöhnk, B., Bayram, Ö., Valerius, O., Braus, G. H., & Riquelme, M. (2015). Dissecting the function of the different chitin synthases in vegetative growth and sexual development in Neurospora crassa. Fungal Genetics and Biology : FG & B, 75, 30-45. https://doi.org/10.1016/j.fgb.2015.01.002
Fajardo-Somera RA, et al. Dissecting the Function of the Different Chitin Synthases in Vegetative Growth and Sexual Development in Neurospora Crassa. Fungal Genet Biol. 2015;75:30-45. PubMed PMID: 25596036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dissecting the function of the different chitin synthases in vegetative growth and sexual development in Neurospora crassa. AU - Fajardo-Somera,Rosa A, AU - Jöhnk,Bastian, AU - Bayram,Özgür, AU - Valerius,Oliver, AU - Braus,Gerhard H, AU - Riquelme,Meritxell, Y1 - 2015/01/13/ PY - 2014/09/30/received PY - 2014/12/17/revised PY - 2015/01/07/accepted PY - 2015/1/18/entrez PY - 2015/1/18/pubmed PY - 2015/8/22/medline KW - Cell wall KW - Chitin synthase KW - Hyphal morphogenesis KW - Proteomics KW - Sexual development KW - Spitzenkörper SP - 30 EP - 45 JF - Fungal genetics and biology : FG & B JO - Fungal Genet. Biol. VL - 75 N2 - Chitin, one of the most important carbohydrates of the fungal cell wall, is synthesized by chitin synthases (CHS). Seven sequences encoding CHSs have been identified in the genome of Neurospora crassa. Previously, CHS-1, -3 and -6 were found at the Spitzenkörper(Spk) core and developing septa. We investigated the functional importance of each CHS in growth and development of N. crassa. The cellular distribution of each CHS tagged with fluorescent proteins and the impact of corresponding gene deletions on vegetative growth and sexual development were compared. CHS-2, -4, -5 and -7 were also found at the core of the Spk and in forming septa in vegetative hyphae. As the septum ring developed, CHS-2-GFP remained at the growing edge of the septum until it localized around the septal pore. In addition, all CHSs were located in cross-walls of conidiophores. A partial co-localization of CHS-1-m and CHS-5-GFP or CHS-2-GFP occurred in the Spk and septa. Analyses of deletion mutants suggested that CHS-6 has a role primarily in hyphal extension and ascospore formation, CHS-5 in aerial hyphae, conidia and ascospore formation, CHS-3 in perithecia development and CHS-7 in all of the aforementioned. We show that chs-7/csmB fulfills a sexual function and chs-6/chsG fulfills a vegetative growth function in N. crassa but not in Aspergillus nidulans, whereas vice versa chs-2/chsA fulfills a sexual function in A. nidulans but not in N. crassa. This suggests that different classes of CHSs can fulfill distinct developmental functions in various fungi. Immunoprecipitation followed by mass spectrometry of CHS-1-GFP, CHS-4-GFP and CHS-5-GFP identified distinct putative interacting proteins for each CHS. Collectively, our results suggest that there are distinct populations of chitosomes, each carrying specific CHSs, with particular roles during different developmental stages. SN - 1096-0937 UR - https://www.unboundmedicine.com/medline/citation/25596036/Dissecting_the_function_of_the_different_chitin_synthases_in_vegetative_growth_and_sexual_development_in_Neurospora_crassa_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1087-1845(15)00005-5 DB - PRIME DP - Unbound Medicine ER -