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Determination and pharmacokinetic study of pirfenidone in rat plasma by UPLC-MS/MS.
J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Feb 15; 981-982:14-8.JC

Abstract

A rapid, sensitive and selective ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed and validated for the determination and pharmacokinetic investigation of pirfenidone in rat plasma. Sample preparation was accomplished through a simple one-step deproteinization procedure with 0.2 mL of acetonitrile to a 0.1 mL plasma sample. Plasma samples were separated by UPLC on an Acquity UPLC BEH C18 column using a mobile phase consisting of acetonitrile-0.1% formic acid in water with gradient elution. The total run time was 3.0 min and the elution of pirfenidone was at 1.39 min. The detection was performed on a triple quadrupole tandem mass spectrometer in the multiple reaction-monitoring (MRM) mode using the respective transitions m/z 186.2→92.1 for pirfenidone and m/z 237.1→194.2 for carbamazepine (IS), respectively. The calibration curve was linear over the range of 5-2000 ng/mL with a lower limit of quantitation (LLOQ) of 5 ng/mL. Mean recovery of pirfenidone in plasma was in the range of 80.4-84.3%. Intra-day and inter-day precision were both <12.1%. This method was successfully applied in pharmacokinetic study after oral administration of 10.0mg/kg pirfenidone in rats.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Sun W
The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Jiang ZL
School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China.
Zhou L
The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Chen RM
The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Wang Z
The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Li WS
Ningbo Municipal Hospital of TCM, Ningbo 315010, China.
Jiang SM
The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Hu GX
School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China.
Chen RJ
The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China. Electronic address: crj@wzhealth.com.

MeSH

AnimalsChromatography, High Pressure LiquidDrug StabilityLimit of DetectionMalePyridonesRatsRats, Sprague-DawleyReproducibility of ResultsTandem Mass Spectrometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25596380

Citation

Sun, Wei, et al. "Determination and Pharmacokinetic Study of Pirfenidone in Rat Plasma By UPLC-MS/MS." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 981-982, 2015, pp. 14-8.
Sun W, Jiang ZL, Zhou L, et al. Determination and pharmacokinetic study of pirfenidone in rat plasma by UPLC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2015;981-982:14-8.
Sun, W., Jiang, Z. L., Zhou, L., Chen, R. M., Wang, Z., Li, W. S., Jiang, S. M., Hu, G. X., & Chen, R. J. (2015). Determination and pharmacokinetic study of pirfenidone in rat plasma by UPLC-MS/MS. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 981-982, 14-8. https://doi.org/10.1016/j.jchromb.2014.12.027
Sun W, et al. Determination and Pharmacokinetic Study of Pirfenidone in Rat Plasma By UPLC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Feb 15;981-982:14-8. PubMed PMID: 25596380.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination and pharmacokinetic study of pirfenidone in rat plasma by UPLC-MS/MS. AU - Sun,Wei, AU - Jiang,Zhe-li, AU - Zhou,Lei, AU - Chen,Rui-min, AU - Wang,Zhe, AU - Li,Wan-shu, AU - Jiang,Shuo-min, AU - Hu,Guo-xin, AU - Chen,Rui-jie, Y1 - 2015/01/03/ PY - 2014/09/26/received PY - 2014/12/18/revised PY - 2014/12/22/accepted PY - 2015/1/18/entrez PY - 2015/1/18/pubmed PY - 2015/9/24/medline KW - Pharmacokinetics KW - Pirfenidone KW - Rat plasma KW - UPLC–MS/MS SP - 14 EP - 8 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 981-982 N2 - A rapid, sensitive and selective ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed and validated for the determination and pharmacokinetic investigation of pirfenidone in rat plasma. Sample preparation was accomplished through a simple one-step deproteinization procedure with 0.2 mL of acetonitrile to a 0.1 mL plasma sample. Plasma samples were separated by UPLC on an Acquity UPLC BEH C18 column using a mobile phase consisting of acetonitrile-0.1% formic acid in water with gradient elution. The total run time was 3.0 min and the elution of pirfenidone was at 1.39 min. The detection was performed on a triple quadrupole tandem mass spectrometer in the multiple reaction-monitoring (MRM) mode using the respective transitions m/z 186.2→92.1 for pirfenidone and m/z 237.1→194.2 for carbamazepine (IS), respectively. The calibration curve was linear over the range of 5-2000 ng/mL with a lower limit of quantitation (LLOQ) of 5 ng/mL. Mean recovery of pirfenidone in plasma was in the range of 80.4-84.3%. Intra-day and inter-day precision were both <12.1%. This method was successfully applied in pharmacokinetic study after oral administration of 10.0mg/kg pirfenidone in rats. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/25596380/Determination_and_pharmacokinetic_study_of_pirfenidone_in_rat_plasma_by_UPLC_MS/MS_ DB - PRIME DP - Unbound Medicine ER -