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Addition of thymidine to culture media for accurate examination of thymidine-dependent small-colony variants of methicillin-resistant Staphylococcus aureus: a pilot study.
J Microbiol Methods 2015; 110:40-4JM

Abstract

Small-colony variants (SCVs) are slow-growing subpopulations of various auxotrophic bacterial strains. Thymidine-dependent SCVs (TD-SCVs) are unable to synthesize thymidine; hence, these variants fail to grow in a medium without thymidine. In this study, we used 10 TD-SCVs of Staphylococcus aureus, of which four strains possessed mecA. We compared the efficacy of a newly modified medium containing thymidine for the detection of TD-SCVs of methicillin-resistant S. aureus (MRSA) to the efficacy of routinely used laboratory media. We observed that none of the 10 TD-SCVs of S. aureus grew in Mueller-Hinton agar, and four TD-SCVs of MRSA failed to grow on all MRSA screening media, except for the ChromID™ MRSA medium. Laboratory tests conducted using medium with thymidine incorporated showed that thymidine did not affect the minimum inhibitory concentrations of oxacillin and cefoxitin for clinical isolates of S. aureus, and was able to detect MRSA, including TD-SCVs. These findings showed that thymidine-incorporated media are able to detect TD-SCVs of MRSA without altering the properties of other clinically isolated MRSA strains.

Authors+Show Affiliations

Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan.Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan. Electronic address: ggatcc@shinshu-u.ac.jp.Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine, Matsumoto, Japan.Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan.Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan.Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan.Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan.Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25596520

Citation

Horiuchi, Kazuki, et al. "Addition of Thymidine to Culture Media for Accurate Examination of Thymidine-dependent Small-colony Variants of Methicillin-resistant Staphylococcus Aureus: a Pilot Study." Journal of Microbiological Methods, vol. 110, 2015, pp. 40-4.
Horiuchi K, Matsumoto T, Ota Y, et al. Addition of thymidine to culture media for accurate examination of thymidine-dependent small-colony variants of methicillin-resistant Staphylococcus aureus: a pilot study. J Microbiol Methods. 2015;110:40-4.
Horiuchi, K., Matsumoto, T., Ota, Y., Kasuga, E., Negishi, T., Yaguchi, T., ... Honda, T. (2015). Addition of thymidine to culture media for accurate examination of thymidine-dependent small-colony variants of methicillin-resistant Staphylococcus aureus: a pilot study. Journal of Microbiological Methods, 110, pp. 40-4. doi:10.1016/j.mimet.2015.01.007.
Horiuchi K, et al. Addition of Thymidine to Culture Media for Accurate Examination of Thymidine-dependent Small-colony Variants of Methicillin-resistant Staphylococcus Aureus: a Pilot Study. J Microbiol Methods. 2015;110:40-4. PubMed PMID: 25596520.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Addition of thymidine to culture media for accurate examination of thymidine-dependent small-colony variants of methicillin-resistant Staphylococcus aureus: a pilot study. AU - Horiuchi,Kazuki, AU - Matsumoto,Takehisa, AU - Ota,Yusuke, AU - Kasuga,Eriko, AU - Negishi,Tatsuya, AU - Yaguchi,Tomomi, AU - Sugano,Mitsutoshi, AU - Honda,Takayuki, Y1 - 2015/01/14/ PY - 2014/10/25/received PY - 2015/01/12/revised PY - 2015/01/13/accepted PY - 2015/1/18/entrez PY - 2015/1/18/pubmed PY - 2015/10/7/medline KW - MRSA screening media KW - TD-SCVs of MRSA KW - Thymidine SP - 40 EP - 4 JF - Journal of microbiological methods JO - J. Microbiol. Methods VL - 110 N2 - Small-colony variants (SCVs) are slow-growing subpopulations of various auxotrophic bacterial strains. Thymidine-dependent SCVs (TD-SCVs) are unable to synthesize thymidine; hence, these variants fail to grow in a medium without thymidine. In this study, we used 10 TD-SCVs of Staphylococcus aureus, of which four strains possessed mecA. We compared the efficacy of a newly modified medium containing thymidine for the detection of TD-SCVs of methicillin-resistant S. aureus (MRSA) to the efficacy of routinely used laboratory media. We observed that none of the 10 TD-SCVs of S. aureus grew in Mueller-Hinton agar, and four TD-SCVs of MRSA failed to grow on all MRSA screening media, except for the ChromID™ MRSA medium. Laboratory tests conducted using medium with thymidine incorporated showed that thymidine did not affect the minimum inhibitory concentrations of oxacillin and cefoxitin for clinical isolates of S. aureus, and was able to detect MRSA, including TD-SCVs. These findings showed that thymidine-incorporated media are able to detect TD-SCVs of MRSA without altering the properties of other clinically isolated MRSA strains. SN - 1872-8359 UR - https://www.unboundmedicine.com/medline/citation/25596520/Addition_of_thymidine_to_culture_media_for_accurate_examination_of_thymidine_dependent_small_colony_variants_of_methicillin_resistant_Staphylococcus_aureus:_a_pilot_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0167-7012(15)00016-0 DB - PRIME DP - Unbound Medicine ER -