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Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine.
Anal Chim Acta 2015; 858:60-5AC

Abstract

A flow injection system incorporated with a polycation-sensitive polymeric membrane electrode in the flow cell is proposed for potentiometric determination of heparin. An external current in nano-ampere scale is continuously applied across the polymeric membrane for controlled release of protamine from the inner filling solution to the sample solution, which makes the electrode membrane regenerate quickly after each measurement. The protamine released at membrane-sample interface is consumed by heparin injected into the flow cell via their strong electrostatic interaction, thus decreasing the measured potential, by which heparin can be detected. Under optimized conditions, a linear relationship between the potential peak height and the concentration of heparin in the sample solution can be obtained in the range of 0.1-2.0 U mL(-1), and the detection limit is 0.06 U mL(-1). The proposed potentiometric sensing system has been successfully applied to the determination of heparin in undiluted sheep whole blood.

Authors+Show Affiliations

Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai, Shandong 264003, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai, Shandong 264003, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai, Shandong 264003, PR China.Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai, Shandong 264003, PR China. Electronic address: wqin@yic.ac.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25597803

Citation

Lei, Jiahong, et al. "Potentiometric Flow Injection Sensing System for Determination of Heparin Based On Current-controlled Release of Protamine." Analytica Chimica Acta, vol. 858, 2015, pp. 60-5.
Lei J, Ding J, Chen Y, et al. Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine. Anal Chim Acta. 2015;858:60-5.
Lei, J., Ding, J., Chen, Y., & Qin, W. (2015). Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine. Analytica Chimica Acta, 858, pp. 60-5. doi:10.1016/j.aca.2014.12.018.
Lei J, et al. Potentiometric Flow Injection Sensing System for Determination of Heparin Based On Current-controlled Release of Protamine. Anal Chim Acta. 2015 Feb 9;858:60-5. PubMed PMID: 25597803.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine. AU - Lei,Jiahong, AU - Ding,Jiawang, AU - Chen,Yan, AU - Qin,Wei, Y1 - 2014/12/11/ PY - 2014/09/26/received PY - 2014/12/04/revised PY - 2014/12/09/accepted PY - 2015/1/20/entrez PY - 2015/1/20/pubmed PY - 2015/9/15/medline KW - Current-controlled release KW - Flow injection analysis KW - Heparin KW - Polycation-sensitive membrane electrodes KW - Potentiometry KW - Protamine SP - 60 EP - 5 JF - Analytica chimica acta JO - Anal. Chim. Acta VL - 858 N2 - A flow injection system incorporated with a polycation-sensitive polymeric membrane electrode in the flow cell is proposed for potentiometric determination of heparin. An external current in nano-ampere scale is continuously applied across the polymeric membrane for controlled release of protamine from the inner filling solution to the sample solution, which makes the electrode membrane regenerate quickly after each measurement. The protamine released at membrane-sample interface is consumed by heparin injected into the flow cell via their strong electrostatic interaction, thus decreasing the measured potential, by which heparin can be detected. Under optimized conditions, a linear relationship between the potential peak height and the concentration of heparin in the sample solution can be obtained in the range of 0.1-2.0 U mL(-1), and the detection limit is 0.06 U mL(-1). The proposed potentiometric sensing system has been successfully applied to the determination of heparin in undiluted sheep whole blood. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/25597803/Potentiometric_flow_injection_sensing_system_for_determination_of_heparin_based_on_current_controlled_release_of_protamine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(14)01419-6 DB - PRIME DP - Unbound Medicine ER -