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Enzyme-assisted extraction of bioactive material from Chondrus crispus and Codium fragile and its effect on herpes simplex virus (HSV-1).
Mar Drugs. 2015 Jan 16; 13(1):558-80.MD

Abstract

Codium fragile and Chondrus crispus are, respectively, green and red seaweeds which are abundant along the North Atlantic coasts. We investigated the chemical composition and antiviral activity of enzymatic extracts of C. fragile (CF) and C. crispus (CC). On a dry weight basis, CF consisted of 11% protein, 31% neutral sugars, 0.8% sulfate, 0.6% uronic acids, and 49% ash, while CC contained 27% protein, 28% neutral sugars, 17% sulfate, 1.8% uronic acids, and 25% ash. Enzyme-assisted hydrolysis improved the extraction efficiency of bioactive materials. Commercial proteases and carbohydrases significantly improved (p ≤ 0.001) biomass yield (40%-70% dry matter) as compared to aqueous extraction (20%-25% dry matter). Moreover, enzymatic hydrolysis enhanced the recovery of protein, neutral sugars, uronic acids, and sulfates. The enzymatic hydrolysates exhibited significant activity against Herpes simplex virus (HSV-1) with EC50 of 77.6-126.8 μg/mL for CC and 36.5-41.3 μg/mL for CF, at a multiplicity of infection (MOI) of 0.001 ID50/cells without cytotoxity (1-200 μg/mL). The extracts obtained from proteases (P1) and carbohydrases (C3) were also effective at higher virus MOI of 0.01 ID50/cells without cytotoxity. Taken together, these results indicate the potential application of enzymatic hydrolysates of C. fragile and C. crispus in functional food and antiviral drug discovery.

Authors+Show Affiliations

Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, PO Box 550, Truro, NS B2N 5E3, Canada. gr784654@dal.ca.Laboratoire de Biotechnologie et Chimie Marines, EA3884, UBS, IUEM, F-56000 Vannes, France. anne-sophie.burlot@univ-ubs.fr.Laboratoire de Biotechnologie et Chimie Marines, EA3884, UBS, IUEM, F-56000 Vannes, France. Christel.Marty@univ-ubs.fr.Acadian Seaplants Limited, 30 Brown Avenue, Dartmouth, NS B3B 1X8, Canada. Alan.Critchley@acadian.ca.Acadian Seaplants Limited, 30 Brown Avenue, Dartmouth, NS B3B 1X8, Canada. jhafting@acadian.ca.Laboratoire de Biotechnologie et Chimie Marines, EA3884, UBS, IUEM, F-56000 Vannes, France. gilles.bedoux@univubs.fr.Laboratoire de Biotechnologie et Chimie Marines, EA3884, UBS, IUEM, F-56000 Vannes, France. nathalie.bourgougnon@univ-ubs.fr.Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, PO Box 550, Truro, NS B2N 5E3, Canada. bprithiviraj@dal.ca.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25603348

Citation

Kulshreshtha, Garima, et al. "Enzyme-assisted Extraction of Bioactive Material From Chondrus Crispus and Codium Fragile and Its Effect On Herpes Simplex Virus (HSV-1)." Marine Drugs, vol. 13, no. 1, 2015, pp. 558-80.
Kulshreshtha G, Burlot AS, Marty C, et al. Enzyme-assisted extraction of bioactive material from Chondrus crispus and Codium fragile and its effect on herpes simplex virus (HSV-1). Mar Drugs. 2015;13(1):558-80.
Kulshreshtha, G., Burlot, A. S., Marty, C., Critchley, A., Hafting, J., Bedoux, G., Bourgougnon, N., & Prithiviraj, B. (2015). Enzyme-assisted extraction of bioactive material from Chondrus crispus and Codium fragile and its effect on herpes simplex virus (HSV-1). Marine Drugs, 13(1), 558-80. https://doi.org/10.3390/md13010558
Kulshreshtha G, et al. Enzyme-assisted Extraction of Bioactive Material From Chondrus Crispus and Codium Fragile and Its Effect On Herpes Simplex Virus (HSV-1). Mar Drugs. 2015 Jan 16;13(1):558-80. PubMed PMID: 25603348.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enzyme-assisted extraction of bioactive material from Chondrus crispus and Codium fragile and its effect on herpes simplex virus (HSV-1). AU - Kulshreshtha,Garima, AU - Burlot,Anne-Sophie, AU - Marty,Christel, AU - Critchley,Alan, AU - Hafting,Jeff, AU - Bedoux,Gilles, AU - Bourgougnon,Nathalie, AU - Prithiviraj,Balakrishnan, Y1 - 2015/01/16/ PY - 2014/11/11/received PY - 2015/01/04/accepted PY - 2015/1/21/entrez PY - 2015/1/21/pubmed PY - 2015/9/19/medline SP - 558 EP - 80 JF - Marine drugs JO - Mar Drugs VL - 13 IS - 1 N2 - Codium fragile and Chondrus crispus are, respectively, green and red seaweeds which are abundant along the North Atlantic coasts. We investigated the chemical composition and antiviral activity of enzymatic extracts of C. fragile (CF) and C. crispus (CC). On a dry weight basis, CF consisted of 11% protein, 31% neutral sugars, 0.8% sulfate, 0.6% uronic acids, and 49% ash, while CC contained 27% protein, 28% neutral sugars, 17% sulfate, 1.8% uronic acids, and 25% ash. Enzyme-assisted hydrolysis improved the extraction efficiency of bioactive materials. Commercial proteases and carbohydrases significantly improved (p ≤ 0.001) biomass yield (40%-70% dry matter) as compared to aqueous extraction (20%-25% dry matter). Moreover, enzymatic hydrolysis enhanced the recovery of protein, neutral sugars, uronic acids, and sulfates. The enzymatic hydrolysates exhibited significant activity against Herpes simplex virus (HSV-1) with EC50 of 77.6-126.8 μg/mL for CC and 36.5-41.3 μg/mL for CF, at a multiplicity of infection (MOI) of 0.001 ID50/cells without cytotoxity (1-200 μg/mL). The extracts obtained from proteases (P1) and carbohydrases (C3) were also effective at higher virus MOI of 0.01 ID50/cells without cytotoxity. Taken together, these results indicate the potential application of enzymatic hydrolysates of C. fragile and C. crispus in functional food and antiviral drug discovery. SN - 1660-3397 UR - https://www.unboundmedicine.com/medline/citation/25603348/Enzyme_assisted_extraction_of_bioactive_material_from_Chondrus_crispus_and_Codium_fragile_and_its_effect_on_herpes_simplex_virus__HSV_1__ L2 - https://www.mdpi.com/resolver?pii=md13010558 DB - PRIME DP - Unbound Medicine ER -