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Real-time electrochemical detection of hydrogen peroxide secretion in live cells by Pt nanoparticles decorated graphene-carbon nanotube hybrid paper electrode.
Biosens Bioelectron. 2015 Jun 15; 68:358-364.BB

Abstract

In this work, we develop a new type of flexible and lightweight electrode based on highly dense Pt nanoparticles decorated free-standing graphene-carbon nanotube (CNT) hybrid paper (Pt/graphene-CNT paper), and explore its practical application as flexible electrochemical biosensor for the real-time tracking hydrogen peroxide (H2O2) secretion by live cells. For the fabrication of flexible nanohybrid electrode, the incorporation of CNT in graphene paper not only improves the electrical conductivity and the mechanical strength of graphene paper, but also increases its surface roughness and provides more nucleation sites for metal nanoparticles. Ultrafine Pt nanoparticles are further decorated on graphene-CNT paper by well controlled sputter deposition method, which offers several advantages such as defined particle size and dispersion, high loading density and strong adhesion between the nanoparticles and the substrate. Consequently, the resultant flexible Pt/graphene-CNT paper electrode demonstrates a variety of desirable electrochemical properties including large electrochemical active surface area, excellent electrocatalytic activity, high stability and exceptional flexibility. When used for nonenzymatic detection of H2O2, Pt/graphene-CNT paper exhibits outstanding sensing performance such as high sensitivity, selectivity, stability and reproducibility. The sensitivity is 1.41 µA µM(-1) cm(-2) with a linear range up to 25 µM and a low detection limit of 10 nM (S/N=3), which enables the resultant biosensor for the real-time tracking H2O2 secretion by live cells macrophages.

Authors+Show Affiliations

School of Material Science and Engineering, Wuhan Institute of Technology, Wuhan, Hubei 430073, People's Republic of China; School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, 637457, Singapore.School of Material Science and Engineering, Wuhan Institute of Technology, Wuhan, Hubei 430073, People's Republic of China.School of Material Science and Engineering, Wuhan Institute of Technology, Wuhan, Hubei 430073, People's Republic of China.School of Material Science and Engineering, Wuhan Institute of Technology, Wuhan, Hubei 430073, People's Republic of China. Electronic address: zhouaijun3@sina.com.School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, 637457, Singapore. Electronic address: hduan@ntu.edu.sg.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25603401

Citation

Sun, Yimin, et al. "Real-time Electrochemical Detection of Hydrogen Peroxide Secretion in Live Cells By Pt Nanoparticles Decorated Graphene-carbon Nanotube Hybrid Paper Electrode." Biosensors & Bioelectronics, vol. 68, 2015, pp. 358-364.
Sun Y, He K, Zhang Z, et al. Real-time electrochemical detection of hydrogen peroxide secretion in live cells by Pt nanoparticles decorated graphene-carbon nanotube hybrid paper electrode. Biosens Bioelectron. 2015;68:358-364.
Sun, Y., He, K., Zhang, Z., Zhou, A., & Duan, H. (2015). Real-time electrochemical detection of hydrogen peroxide secretion in live cells by Pt nanoparticles decorated graphene-carbon nanotube hybrid paper electrode. Biosensors & Bioelectronics, 68, 358-364. https://doi.org/10.1016/j.bios.2015.01.017
Sun Y, et al. Real-time Electrochemical Detection of Hydrogen Peroxide Secretion in Live Cells By Pt Nanoparticles Decorated Graphene-carbon Nanotube Hybrid Paper Electrode. Biosens Bioelectron. 2015 Jun 15;68:358-364. PubMed PMID: 25603401.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Real-time electrochemical detection of hydrogen peroxide secretion in live cells by Pt nanoparticles decorated graphene-carbon nanotube hybrid paper electrode. AU - Sun,Yimin, AU - He,Kui, AU - Zhang,Zefen, AU - Zhou,Aijun, AU - Duan,Hongwei, Y1 - 2015/01/08/ PY - 2014/10/29/received PY - 2014/12/26/revised PY - 2015/01/07/accepted PY - 2015/1/21/entrez PY - 2015/1/21/pubmed PY - 2015/12/15/medline KW - Cell tracking KW - Flexible electrochemical biosensor KW - Graphene–carbon nanotube hybrid paper KW - Nonenzymatic hydrogen peroxide detection KW - Pt nanoparticles KW - Sputter deposition SP - 358 EP - 364 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 68 N2 - In this work, we develop a new type of flexible and lightweight electrode based on highly dense Pt nanoparticles decorated free-standing graphene-carbon nanotube (CNT) hybrid paper (Pt/graphene-CNT paper), and explore its practical application as flexible electrochemical biosensor for the real-time tracking hydrogen peroxide (H2O2) secretion by live cells. For the fabrication of flexible nanohybrid electrode, the incorporation of CNT in graphene paper not only improves the electrical conductivity and the mechanical strength of graphene paper, but also increases its surface roughness and provides more nucleation sites for metal nanoparticles. Ultrafine Pt nanoparticles are further decorated on graphene-CNT paper by well controlled sputter deposition method, which offers several advantages such as defined particle size and dispersion, high loading density and strong adhesion between the nanoparticles and the substrate. Consequently, the resultant flexible Pt/graphene-CNT paper electrode demonstrates a variety of desirable electrochemical properties including large electrochemical active surface area, excellent electrocatalytic activity, high stability and exceptional flexibility. When used for nonenzymatic detection of H2O2, Pt/graphene-CNT paper exhibits outstanding sensing performance such as high sensitivity, selectivity, stability and reproducibility. The sensitivity is 1.41 µA µM(-1) cm(-2) with a linear range up to 25 µM and a low detection limit of 10 nM (S/N=3), which enables the resultant biosensor for the real-time tracking H2O2 secretion by live cells macrophages. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/25603401/Real_time_electrochemical_detection_of_hydrogen_peroxide_secretion_in_live_cells_by_Pt_nanoparticles_decorated_graphene_carbon_nanotube_hybrid_paper_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(15)00018-4 DB - PRIME DP - Unbound Medicine ER -