Tags

Type your tag names separated by a space and hit enter

Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells.
Invest Ophthalmol Vis Sci. 2015 Jan 20; 56(2):1051-62.IO

Abstract

PURPOSE

The purpose of this study was to determine whether human retinal pigment epithelial (RPE) cells from induced pluripotent stem (iPS) cells could inhibit T-cell activation in vitro.

METHODS

Cultured iPS-derived RPE (iPS-RPE) cells were established from fresh skin tissues or dental pulp cells obtained from healthy donors or a retinal patient after informed consent was obtained. To confirm expression of the specific markers on iPS and iPS-RPE cells, immunohistochemistry, quantitative RT-PCR (qRT-PCR), and flow cytometry were performed. Target T cells were obtained from peripheral blood mononuclear cells of healthy donors. Target T cells were assessed for proliferation by incorporation of bromodeoxyuridine or carboxyfluorescein succinimidyl ester for production of cytokines such as IFN-γ. Expression of TGFβ and other candidate molecules by iPS-RPE cells was evaluated with flow cytometry, ELISA, multiplex cytokine array, immunohistochemistry, and qRT-PCR.

RESULTS

The RPE cells we established from iPS cells had many characteristics of mature RPE cells but no characteristics of pluripotent stem cells. Cultured iPS-RPE cells inhibited cell proliferation and production of IFN-γ by activated CD4(+) T cells. In some bystander T cells, iPS-derived RPE cells induced CD25(+)Foxp3(+) regulatory T cells in vitro. Induced pluripotent stem-RPE cells constitutively expressed TGFβ and suppressed activation of T cells via soluble TGFβ, because TGFβ-downregulated iPS-RPE cells did not inhibit this T-cell activation.

CONCLUSIONS

Cultured iPS-derived retinal cells fully suppress T-cell activation. Transplantation of iPS-RPE cells into the eye might be a therapy for retinal disorders.

Authors+Show Affiliations

Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe, Japan.Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe, Japan.Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe, Japan.Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe, Japan Department of Physiology, Keio University School of Medicine, Tokyo, Japan.Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe, Japan.Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe, Japan.Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe, Japan.Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe, Japan.Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25604685

Citation

Sugita, Sunao, et al. "Inhibition of T-cell Activation By Retinal Pigment Epithelial Cells Derived From Induced Pluripotent Stem Cells." Investigative Ophthalmology & Visual Science, vol. 56, no. 2, 2015, pp. 1051-62.
Sugita S, Kamao H, Iwasaki Y, et al. Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells. Invest Ophthalmol Vis Sci. 2015;56(2):1051-62.
Sugita, S., Kamao, H., Iwasaki, Y., Okamoto, S., Hashiguchi, T., Iseki, K., Hayashi, N., Mandai, M., & Takahashi, M. (2015). Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells. Investigative Ophthalmology & Visual Science, 56(2), 1051-62. https://doi.org/10.1167/iovs.14-15619
Sugita S, et al. Inhibition of T-cell Activation By Retinal Pigment Epithelial Cells Derived From Induced Pluripotent Stem Cells. Invest Ophthalmol Vis Sci. 2015 Jan 20;56(2):1051-62. PubMed PMID: 25604685.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells. AU - Sugita,Sunao, AU - Kamao,Hiroyuki, AU - Iwasaki,Yuko, AU - Okamoto,Satoshi, AU - Hashiguchi,Tomoyo, AU - Iseki,Kyoko, AU - Hayashi,Naoko, AU - Mandai,Michiko, AU - Takahashi,Masayo, Y1 - 2015/01/20/ PY - 2015/1/22/entrez PY - 2015/1/22/pubmed PY - 2015/3/25/medline KW - PS cells KW - TGFβ KW - cytokine SP - 1051 EP - 62 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 56 IS - 2 N2 - PURPOSE: The purpose of this study was to determine whether human retinal pigment epithelial (RPE) cells from induced pluripotent stem (iPS) cells could inhibit T-cell activation in vitro. METHODS: Cultured iPS-derived RPE (iPS-RPE) cells were established from fresh skin tissues or dental pulp cells obtained from healthy donors or a retinal patient after informed consent was obtained. To confirm expression of the specific markers on iPS and iPS-RPE cells, immunohistochemistry, quantitative RT-PCR (qRT-PCR), and flow cytometry were performed. Target T cells were obtained from peripheral blood mononuclear cells of healthy donors. Target T cells were assessed for proliferation by incorporation of bromodeoxyuridine or carboxyfluorescein succinimidyl ester for production of cytokines such as IFN-γ. Expression of TGFβ and other candidate molecules by iPS-RPE cells was evaluated with flow cytometry, ELISA, multiplex cytokine array, immunohistochemistry, and qRT-PCR. RESULTS: The RPE cells we established from iPS cells had many characteristics of mature RPE cells but no characteristics of pluripotent stem cells. Cultured iPS-RPE cells inhibited cell proliferation and production of IFN-γ by activated CD4(+) T cells. In some bystander T cells, iPS-derived RPE cells induced CD25(+)Foxp3(+) regulatory T cells in vitro. Induced pluripotent stem-RPE cells constitutively expressed TGFβ and suppressed activation of T cells via soluble TGFβ, because TGFβ-downregulated iPS-RPE cells did not inhibit this T-cell activation. CONCLUSIONS: Cultured iPS-derived retinal cells fully suppress T-cell activation. Transplantation of iPS-RPE cells into the eye might be a therapy for retinal disorders. SN - 1552-5783 UR - https://www.unboundmedicine.com/medline/citation/25604685/Inhibition_of_T_cell_activation_by_retinal_pigment_epithelial_cells_derived_from_induced_pluripotent_stem_cells_ L2 - https://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.14-15619 DB - PRIME DP - Unbound Medicine ER -