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Reduced Coenzyme Q10 Decreases Urinary 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine Concentrations in Healthy Young Female Subjects.
J Med Food. 2015 Aug; 18(8):835-40.JM

Abstract

It remains unclear whether dietary supplementation with coenzyme Q10 (CoQ10) provides beneficial effects for healthy individuals, especially young subjects. This study investigated the effects of dietary supplementation with CoQ10 on oxidative stress in healthy young females. We performed a placebo-controlled trial using a crossover design (n=28) with 100 mg/day CoQ10 in reduced form or placebo, each lasting 2 weeks with a 2-week interval. The urinary levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), a biomarker of oxidative DNA damage, were determined by high-performance liquid chromatography (HPLC) coupled to an electrochemical detector. Levels of malondialdehyde (MDA), a biomarker of lipid peroxidation, and antioxidant vitamin C in urine were also measured using a thiobarbituric acid-reactive substance method with a commercial kit and by the 2,4-dinitrophenylhydrazine method with HPLC, respectively. Urinary 8-oxodG levels during supplementation with reduced form of CoQ10 (median [first and third quartiles]: 1.76 [1.24-2.08] nmol/mmol creatinine) were significantly lower than those with placebo (2.00 [1.34-2.49] nmol/mmol creatinine, P=.031 by Student's paired t-test using the logarithmically transformed values). In contrast, the urinary levels of MDA and vitamin C were not significantly affected (P=.094 and P=.247 by Student's paired t-test, respectively). There was no evidence of any side effects. Supplementation with CoQ10 in the reduced form showed a slightly protective effect against oxidative DNA damage even in healthy young subjects.

Authors+Show Affiliations

1 Department of Life Science, Tsu City College , Tsu, Mie, Japan .2 Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine , Tsu, Mie, Japan .2 Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine , Tsu, Mie, Japan .2 Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine , Tsu, Mie, Japan .2 Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine , Tsu, Mie, Japan .

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25607850

Citation

Ito, Kimiko, et al. "Reduced Coenzyme Q10 Decreases Urinary 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine Concentrations in Healthy Young Female Subjects." Journal of Medicinal Food, vol. 18, no. 8, 2015, pp. 835-40.
Ito K, Watanabe C, Nakamura A, et al. Reduced Coenzyme Q10 Decreases Urinary 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine Concentrations in Healthy Young Female Subjects. J Med Food. 2015;18(8):835-40.
Ito, K., Watanabe, C., Nakamura, A., Oikawa-Tada, S., & Murata, M. (2015). Reduced Coenzyme Q10 Decreases Urinary 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine Concentrations in Healthy Young Female Subjects. Journal of Medicinal Food, 18(8), 835-40. https://doi.org/10.1089/jmf.2014.3302
Ito K, et al. Reduced Coenzyme Q10 Decreases Urinary 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine Concentrations in Healthy Young Female Subjects. J Med Food. 2015;18(8):835-40. PubMed PMID: 25607850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reduced Coenzyme Q10 Decreases Urinary 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine Concentrations in Healthy Young Female Subjects. AU - Ito,Kimiko, AU - Watanabe,Chigusa, AU - Nakamura,Akari, AU - Oikawa-Tada,Saeko, AU - Murata,Mariko, Y1 - 2015/01/21/ PY - 2015/1/22/entrez PY - 2015/1/22/pubmed PY - 2016/6/29/medline KW - 8-oxo-7,8-dihydro-2′-deoxyguanosine KW - coenzyme Q10 KW - malondialdehyde SP - 835 EP - 40 JF - Journal of medicinal food JO - J Med Food VL - 18 IS - 8 N2 - It remains unclear whether dietary supplementation with coenzyme Q10 (CoQ10) provides beneficial effects for healthy individuals, especially young subjects. This study investigated the effects of dietary supplementation with CoQ10 on oxidative stress in healthy young females. We performed a placebo-controlled trial using a crossover design (n=28) with 100 mg/day CoQ10 in reduced form or placebo, each lasting 2 weeks with a 2-week interval. The urinary levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), a biomarker of oxidative DNA damage, were determined by high-performance liquid chromatography (HPLC) coupled to an electrochemical detector. Levels of malondialdehyde (MDA), a biomarker of lipid peroxidation, and antioxidant vitamin C in urine were also measured using a thiobarbituric acid-reactive substance method with a commercial kit and by the 2,4-dinitrophenylhydrazine method with HPLC, respectively. Urinary 8-oxodG levels during supplementation with reduced form of CoQ10 (median [first and third quartiles]: 1.76 [1.24-2.08] nmol/mmol creatinine) were significantly lower than those with placebo (2.00 [1.34-2.49] nmol/mmol creatinine, P=.031 by Student's paired t-test using the logarithmically transformed values). In contrast, the urinary levels of MDA and vitamin C were not significantly affected (P=.094 and P=.247 by Student's paired t-test, respectively). There was no evidence of any side effects. Supplementation with CoQ10 in the reduced form showed a slightly protective effect against oxidative DNA damage even in healthy young subjects. SN - 1557-7600 UR - https://www.unboundmedicine.com/medline/citation/25607850/Reduced_Coenzyme_Q10_Decreases_Urinary_8_Oxo_78_Dihydro_2'_Deoxyguanosine_Concentrations_in_Healthy_Young_Female_Subjects_ L2 - https://www.liebertpub.com/doi/10.1089/jmf.2014.3302?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -