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Molecular characterization of a new wheat-Thinopyrum intermedium translocation line with resistance to powdery mildew and stripe rust.
Int J Mol Sci. 2015 Jan 20; 16(1):2162-73.IJ

Abstract

A new wheat-Thinopyrum translocation line CH13-21 was selected from the progenies derived from a cross between wheat-Th. intermedium partial amphiploid TAI7047 and wheat line Mianyang11. CH13-21 was characterized by using genomic in situ hybridization (GISH), multicolor-GISH (mc-GISH), multicolor-fluorescence in situ hybridization (mc-FISH) and chromosome-specific molecular markers. When inoculated with stripe rust and powdery mildew isolates, CH13-21 displayed novel resistance to powdery mildew and stripe rust which inherited from its Thinopyrum parent. The chromosomal counting analyses indicated that CH13-21 has 42 chromosomes, with normal bivalent pairing at metaphase I of meiosis. GISH probed by Th. intermedium genomic DNA showed that CH13-21 contained a pair of wheat-Th. intermedium translocated chromosomes. Sequential mc-FISH analyses probed by pSc119.2 and pAs1 clearly revealed that chromosome arm 6BS of CH13-21 was replaced by Thinopyrum chromatin in the translocation chromosome. The molecular markers analysis further confirmed that the introduced Th. intermedium chromatin in CH13-21 belonged to the long arm of homoeologous group 6 chromosome. Therefore, CH13-21 was a new T6BS.6Ai#1L compensating Robertsonian translocation line. It concludes that CH13-21 is a new genetic resource for wheat breeding programs providing novel variation for disease resistances.

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Authors+Show Affiliations

Zhan H
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. zhan030006@126.com.
Zhang X
Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China. zxjemail@163.com.
Li G
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. ligr28@uestc.edu.cn.
Pan Z
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. pzh1990@aliyun.com.
Hu J
Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China. hujing56@126.com.
Li X
Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China. leexinlee@aliyun.com.
Qiao L
Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China. qiaoly1988@126.com.
Jia J
College of agronomy, Shanxi Agricultural University, Taigu 030801, China. jiajuqing@126.com.
Guo H
Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China. zhanhaixian@aliyun.com.
Chang Z
Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China. wrczj@126.com.
Yang Z
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. yangzujun@uestc.edu.cn.

MeSH

AscomycotaChromosomes, PlantDisease ResistanceGenetic MarkersGenome, PlantIn Situ Hybridization, FluorescencePlant DiseasesPolymerase Chain ReactionTranslocation, GeneticTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25608651

Citation

Zhan, Haixian, et al. "Molecular Characterization of a New wheat-Thinopyrum Intermedium Translocation Line With Resistance to Powdery Mildew and Stripe Rust." International Journal of Molecular Sciences, vol. 16, no. 1, 2015, pp. 2162-73.
Zhan H, Zhang X, Li G, et al. Molecular characterization of a new wheat-Thinopyrum intermedium translocation line with resistance to powdery mildew and stripe rust. Int J Mol Sci. 2015;16(1):2162-73.
Zhan, H., Zhang, X., Li, G., Pan, Z., Hu, J., Li, X., Qiao, L., Jia, J., Guo, H., Chang, Z., & Yang, Z. (2015). Molecular characterization of a new wheat-Thinopyrum intermedium translocation line with resistance to powdery mildew and stripe rust. International Journal of Molecular Sciences, 16(1), 2162-73. https://doi.org/10.3390/ijms16012162
Zhan H, et al. Molecular Characterization of a New wheat-Thinopyrum Intermedium Translocation Line With Resistance to Powdery Mildew and Stripe Rust. Int J Mol Sci. 2015 Jan 20;16(1):2162-73. PubMed PMID: 25608651.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of a new wheat-Thinopyrum intermedium translocation line with resistance to powdery mildew and stripe rust. AU - Zhan,Haixian, AU - Zhang,Xiaojun, AU - Li,Guangrong, AU - Pan,Zhihui, AU - Hu,Jin, AU - Li,Xin, AU - Qiao,Linyi, AU - Jia,Juqing, AU - Guo,Huijuan, AU - Chang,Zhijian, AU - Yang,Zujun, Y1 - 2015/01/20/ PY - 2014/12/08/received PY - 2015/01/07/revised PY - 2015/01/09/accepted PY - 2015/1/23/entrez PY - 2015/1/23/pubmed PY - 2015/9/17/medline SP - 2162 EP - 73 JF - International journal of molecular sciences JO - Int J Mol Sci VL - 16 IS - 1 N2 - A new wheat-Thinopyrum translocation line CH13-21 was selected from the progenies derived from a cross between wheat-Th. intermedium partial amphiploid TAI7047 and wheat line Mianyang11. CH13-21 was characterized by using genomic in situ hybridization (GISH), multicolor-GISH (mc-GISH), multicolor-fluorescence in situ hybridization (mc-FISH) and chromosome-specific molecular markers. When inoculated with stripe rust and powdery mildew isolates, CH13-21 displayed novel resistance to powdery mildew and stripe rust which inherited from its Thinopyrum parent. The chromosomal counting analyses indicated that CH13-21 has 42 chromosomes, with normal bivalent pairing at metaphase I of meiosis. GISH probed by Th. intermedium genomic DNA showed that CH13-21 contained a pair of wheat-Th. intermedium translocated chromosomes. Sequential mc-FISH analyses probed by pSc119.2 and pAs1 clearly revealed that chromosome arm 6BS of CH13-21 was replaced by Thinopyrum chromatin in the translocation chromosome. The molecular markers analysis further confirmed that the introduced Th. intermedium chromatin in CH13-21 belonged to the long arm of homoeologous group 6 chromosome. Therefore, CH13-21 was a new T6BS.6Ai#1L compensating Robertsonian translocation line. It concludes that CH13-21 is a new genetic resource for wheat breeding programs providing novel variation for disease resistances. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/25608651/Molecular_characterization_of_a_new_wheat_Thinopyrum_intermedium_translocation_line_with_resistance_to_powdery_mildew_and_stripe_rust_ DB - PRIME DP - Unbound Medicine ER -
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