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Field-evolved resistance to imidacloprid and ethiprole in populations of brown planthopper Nilaparvata lugens collected from across South and East Asia.
Pest Manag Sci. 2016 Jan; 72(1):140-9.PM

Abstract

BACKGROUND

We report on the status of imidacloprid and ethiprole resistance in Nilaparvata lugens Stål collected from across South and East Asia over the period 2005-2012.

RESULTS

A resistance survey found that field populations had developed up to 220-fold resistance to imidacloprid and 223-fold resistance to ethiprole, and that many of the strains collected showed high levels of resistance to both insecticides. We also found that the cytochrome P450 CYP6ER1 was significantly overexpressed in 12 imidacloprid-resistant populations tested when compared with a laboratory susceptible strain, with fold changes ranging from ten- to 90-fold. In contrast, another cytochrome P450 CYP6AY1, also implicated in imidacloprid resistance, was underexpressed in ten of the populations and only significantly overexpressed (3.5-fold) in a single population from India compared with the same susceptible strain. Further selection of two of the imidacloprid-resistant field strains correlated with an approximate threefold increase in expression of CYP6ER1.

CONCLUSIONS

We conclude that overexpression of CYP6ER1 is associated with field-evolved resistance to imidacloprid in brown planthopper populations in five countries in South and East Asia.

Links

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Authors+Show Affiliations

Garrood WT
Biological Chemistry and Crop Protection Department, Rothamsted Research, Harpenden, Hertfordshire, UK.
Zimmer CT
Biological Chemistry and Crop Protection Department, Rothamsted Research, Harpenden, Hertfordshire, UK.
Gorman KJ
Biological Chemistry and Crop Protection Department, Rothamsted Research, Harpenden, Hertfordshire, UK. Oxitec Ltd, Oxford, UK.
Nauen R
Bayer CropScience AG, Pest Control Biology, Monheim, Germany.
Bass C
Biological Chemistry and Crop Protection Department, Rothamsted Research, Harpenden, Hertfordshire, UK.
Davies TG
Biological Chemistry and Crop Protection Department, Rothamsted Research, Harpenden, Hertfordshire, UK.

MeSH

AnimalsAsia, SoutheasternCytochrome P-450 Enzyme SystemAsia, EasternFemaleHemipteraImidazolesIndiaInsecticide ResistanceInsecticidesNeonicotinoidsNitro CompoundsPyrazoles

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25612154

Citation

Garrood, William T., et al. "Field-evolved Resistance to Imidacloprid and Ethiprole in Populations of Brown Planthopper Nilaparvata Lugens Collected From Across South and East Asia." Pest Management Science, vol. 72, no. 1, 2016, pp. 140-9.
Garrood WT, Zimmer CT, Gorman KJ, et al. Field-evolved resistance to imidacloprid and ethiprole in populations of brown planthopper Nilaparvata lugens collected from across South and East Asia. Pest Manag Sci. 2016;72(1):140-9.
Garrood, W. T., Zimmer, C. T., Gorman, K. J., Nauen, R., Bass, C., & Davies, T. G. (2016). Field-evolved resistance to imidacloprid and ethiprole in populations of brown planthopper Nilaparvata lugens collected from across South and East Asia. Pest Management Science, 72(1), 140-9. https://doi.org/10.1002/ps.3980
Garrood WT, et al. Field-evolved Resistance to Imidacloprid and Ethiprole in Populations of Brown Planthopper Nilaparvata Lugens Collected From Across South and East Asia. Pest Manag Sci. 2016;72(1):140-9. PubMed PMID: 25612154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Field-evolved resistance to imidacloprid and ethiprole in populations of brown planthopper Nilaparvata lugens collected from across South and East Asia. AU - Garrood,William T, AU - Zimmer,Christoph T, AU - Gorman,Kevin J, AU - Nauen,Ralf, AU - Bass,Chris, AU - Davies,Thomas G E, Y1 - 2015/02/19/ PY - 2014/11/11/received PY - 2015/01/19/revised PY - 2015/01/19/accepted PY - 2015/1/23/entrez PY - 2015/1/23/pubmed PY - 2016/10/13/medline KW - brown planthopper KW - cytochrome P450 KW - insecticides KW - resistance SP - 140 EP - 9 JF - Pest management science JO - Pest Manag Sci VL - 72 IS - 1 N2 - BACKGROUND: We report on the status of imidacloprid and ethiprole resistance in Nilaparvata lugens Stål collected from across South and East Asia over the period 2005-2012. RESULTS: A resistance survey found that field populations had developed up to 220-fold resistance to imidacloprid and 223-fold resistance to ethiprole, and that many of the strains collected showed high levels of resistance to both insecticides. We also found that the cytochrome P450 CYP6ER1 was significantly overexpressed in 12 imidacloprid-resistant populations tested when compared with a laboratory susceptible strain, with fold changes ranging from ten- to 90-fold. In contrast, another cytochrome P450 CYP6AY1, also implicated in imidacloprid resistance, was underexpressed in ten of the populations and only significantly overexpressed (3.5-fold) in a single population from India compared with the same susceptible strain. Further selection of two of the imidacloprid-resistant field strains correlated with an approximate threefold increase in expression of CYP6ER1. CONCLUSIONS: We conclude that overexpression of CYP6ER1 is associated with field-evolved resistance to imidacloprid in brown planthopper populations in five countries in South and East Asia. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/25612154/Field_evolved_resistance_to_imidacloprid_and_ethiprole_in_populations_of_brown_planthopper_Nilaparvata_lugens_collected_from_across_South_and_East_Asia_ DB - PRIME DP - Unbound Medicine ER -