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Rutin attenuates gentamicin-induced renal damage by reducing oxidative stress, inflammation, apoptosis, and autophagy in rats.
Ren Fail. 2015 Apr; 37(3):518-25.RF

Abstract

Gentamicin is commonly used against gram-negative microorganisms. Its therapeutic use is mainly limited by nephrotoxicity. This study was aimed at evaluating the effect of rutin on oxidative stress, inflammation, apoptosis, and autophagy in gentamicin-induced nephrotoxicity in rats. The rats were treated with saline intraperitoneally (group I), 150 mg/kg of rutin orally (group II), 80 mg/kg of gentamicin intraperitoneally for 8 d (group III), or 150 mg/kg of rutin plus 80 mg/kg of gentamicin (group IV). The serum urea, creatinine, kidney malondialdehyde (MDA), and reduced glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity and protein concentration were measured, and renal histopathology analysis and immunohistochemical staining were performed. Rutin pretreatment attenuated nephrotoxicity induced by gentamicin by reducing the urea, creatinine, and MDA levels and increasing the SOD, CAT, and GPx activity, and the GSH levels. The rutin also inhibited inducible nitric oxide synthase (iNOS), cleaved caspase-3 and light chain 3B (LC3B), as evidenced by immunohistochemical staining. The present study demonstrates that rutin exhibits antioxidant, anti-inflammatory, anti-apoptotic, and anti-autophagic effects and that it attenuates gentamicin-induced nephrotoxicity in rats.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University , Yakutiye, Erzurum , Turkey .No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25613739

Citation

Kandemir, Fatih Mehmet, et al. "Rutin Attenuates Gentamicin-induced Renal Damage By Reducing Oxidative Stress, Inflammation, Apoptosis, and Autophagy in Rats." Renal Failure, vol. 37, no. 3, 2015, pp. 518-25.
Kandemir FM, Ozkaraca M, Yildirim BA, et al. Rutin attenuates gentamicin-induced renal damage by reducing oxidative stress, inflammation, apoptosis, and autophagy in rats. Ren Fail. 2015;37(3):518-25.
Kandemir, F. M., Ozkaraca, M., Yildirim, B. A., Hanedan, B., Kirbas, A., Kilic, K., Aktas, E., & Benzer, F. (2015). Rutin attenuates gentamicin-induced renal damage by reducing oxidative stress, inflammation, apoptosis, and autophagy in rats. Renal Failure, 37(3), 518-25. https://doi.org/10.3109/0886022X.2015.1006100
Kandemir FM, et al. Rutin Attenuates Gentamicin-induced Renal Damage By Reducing Oxidative Stress, Inflammation, Apoptosis, and Autophagy in Rats. Ren Fail. 2015;37(3):518-25. PubMed PMID: 25613739.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rutin attenuates gentamicin-induced renal damage by reducing oxidative stress, inflammation, apoptosis, and autophagy in rats. AU - Kandemir,Fatih Mehmet, AU - Ozkaraca,Mustafa, AU - Yildirim,Betul Apaydin, AU - Hanedan,Basak, AU - Kirbas,Akin, AU - Kilic,Kubra, AU - Aktas,Esra, AU - Benzer,Fulya, Y1 - 2015/01/23/ PY - 2015/1/24/entrez PY - 2015/1/24/pubmed PY - 2016/2/5/medline KW - Gentamicin KW - apoptosis KW - autophagy KW - nephrotoxicity KW - oxidative stress KW - rutin SP - 518 EP - 25 JF - Renal failure JO - Ren Fail VL - 37 IS - 3 N2 - Gentamicin is commonly used against gram-negative microorganisms. Its therapeutic use is mainly limited by nephrotoxicity. This study was aimed at evaluating the effect of rutin on oxidative stress, inflammation, apoptosis, and autophagy in gentamicin-induced nephrotoxicity in rats. The rats were treated with saline intraperitoneally (group I), 150 mg/kg of rutin orally (group II), 80 mg/kg of gentamicin intraperitoneally for 8 d (group III), or 150 mg/kg of rutin plus 80 mg/kg of gentamicin (group IV). The serum urea, creatinine, kidney malondialdehyde (MDA), and reduced glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity and protein concentration were measured, and renal histopathology analysis and immunohistochemical staining were performed. Rutin pretreatment attenuated nephrotoxicity induced by gentamicin by reducing the urea, creatinine, and MDA levels and increasing the SOD, CAT, and GPx activity, and the GSH levels. The rutin also inhibited inducible nitric oxide synthase (iNOS), cleaved caspase-3 and light chain 3B (LC3B), as evidenced by immunohistochemical staining. The present study demonstrates that rutin exhibits antioxidant, anti-inflammatory, anti-apoptotic, and anti-autophagic effects and that it attenuates gentamicin-induced nephrotoxicity in rats. SN - 1525-6049 UR - https://www.unboundmedicine.com/medline/citation/25613739/Rutin_attenuates_gentamicin_induced_renal_damage_by_reducing_oxidative_stress_inflammation_apoptosis_and_autophagy_in_rats_ L2 - https://www.tandfonline.com/doi/full/10.3109/0886022X.2015.1006100 DB - PRIME DP - Unbound Medicine ER -