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Cyclodextrin functionalized graphene-gold nanoparticle hybrids with strong supramolecular capability for electrochemical thrombin aptasensor.
Biosens Bioelectron. 2015 Jun 15; 68:429-436.BB

Abstract

We demonstrate a facile one-pot synthetic strategy for controlled synthesis of thio-β-cyclodextrin functionalized graphene/gold nanoparticles (SH-β-CD-Gr/AuNPs) composites using SH-β-CD as both the dispersant and linker. The obtained SH-β-CD-Gr/AuNPs integrate the excellent electrical properties and large surface area of graphene and AuNPs with supramolecular recognition ability of CD, which show more effective electron transfer and higher enriched ability for the ferrocene probe via the host-guest interaction between CD and ferrocene than SH-β-CD-Gr. In the presence of target, the stronger interaction between aptamer and target makes the ferrocene move closer to the electrode surface, thus facilitating the electron transfer. Based on this sensing mechanism, a new and highly sensitive biosensing concept by the use of SH-β-CD-Gr/AuNPs as enhancing materials is demonstrated for "signal-on" detection of targets (thrombin as a model target). This biosensor exhibits a wide linear range for thrombin from 1.6×10(-17) M to 8.0×10(-15) M and a very low limit of detection 5.2×10(-18) M, which is two-order magnitude better than those of SH-β-CD-Gr (the detection linear range from 1.6×10(-15) M to 8.0×10(-13) M and detection limit of 1.0×10(-15) M). Our proposed electrochemical aptasensor based on SH-β-CD-Gr/AuNPs shows good selectivity against other proteins such as human serum albumin, lysozyme and insulin. To the best of our knowledge, the present SH-β-CD-Gr/AuNPs hybrids are the most efficient graphene-based electrochemical active probes ever reported for biosensors.

Authors+Show Affiliations

Institute of Environmental Science, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.Institute of Environmental Science, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.Institute of Environmental Science, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. Electronic address: guoyj@sxu.edu.cn.Physical Chemistry and Applied Spectroscopy, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25618374

Citation

Xue, Qiong, et al. "Cyclodextrin Functionalized Graphene-gold Nanoparticle Hybrids With Strong Supramolecular Capability for Electrochemical Thrombin Aptasensor." Biosensors & Bioelectronics, vol. 68, 2015, pp. 429-436.
Xue Q, Liu Z, Guo Y, et al. Cyclodextrin functionalized graphene-gold nanoparticle hybrids with strong supramolecular capability for electrochemical thrombin aptasensor. Biosens Bioelectron. 2015;68:429-436.
Xue, Q., Liu, Z., Guo, Y., & Guo, S. (2015). Cyclodextrin functionalized graphene-gold nanoparticle hybrids with strong supramolecular capability for electrochemical thrombin aptasensor. Biosensors & Bioelectronics, 68, 429-436. https://doi.org/10.1016/j.bios.2015.01.025
Xue Q, et al. Cyclodextrin Functionalized Graphene-gold Nanoparticle Hybrids With Strong Supramolecular Capability for Electrochemical Thrombin Aptasensor. Biosens Bioelectron. 2015 Jun 15;68:429-436. PubMed PMID: 25618374.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cyclodextrin functionalized graphene-gold nanoparticle hybrids with strong supramolecular capability for electrochemical thrombin aptasensor. AU - Xue,Qiong, AU - Liu,Zhiguang, AU - Guo,Yujing, AU - Guo,Shaojun, Y1 - 2015/01/12/ PY - 2014/09/20/received PY - 2015/01/09/revised PY - 2015/01/10/accepted PY - 2015/1/26/entrez PY - 2015/1/27/pubmed PY - 2015/12/15/medline KW - Aptasensor KW - Gold nanoparticle KW - Graphene KW - Supramolecular recognition KW - Thio-β-cyclodextrin SP - 429 EP - 436 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 68 N2 - We demonstrate a facile one-pot synthetic strategy for controlled synthesis of thio-β-cyclodextrin functionalized graphene/gold nanoparticles (SH-β-CD-Gr/AuNPs) composites using SH-β-CD as both the dispersant and linker. The obtained SH-β-CD-Gr/AuNPs integrate the excellent electrical properties and large surface area of graphene and AuNPs with supramolecular recognition ability of CD, which show more effective electron transfer and higher enriched ability for the ferrocene probe via the host-guest interaction between CD and ferrocene than SH-β-CD-Gr. In the presence of target, the stronger interaction between aptamer and target makes the ferrocene move closer to the electrode surface, thus facilitating the electron transfer. Based on this sensing mechanism, a new and highly sensitive biosensing concept by the use of SH-β-CD-Gr/AuNPs as enhancing materials is demonstrated for "signal-on" detection of targets (thrombin as a model target). This biosensor exhibits a wide linear range for thrombin from 1.6×10(-17) M to 8.0×10(-15) M and a very low limit of detection 5.2×10(-18) M, which is two-order magnitude better than those of SH-β-CD-Gr (the detection linear range from 1.6×10(-15) M to 8.0×10(-13) M and detection limit of 1.0×10(-15) M). Our proposed electrochemical aptasensor based on SH-β-CD-Gr/AuNPs shows good selectivity against other proteins such as human serum albumin, lysozyme and insulin. To the best of our knowledge, the present SH-β-CD-Gr/AuNPs hybrids are the most efficient graphene-based electrochemical active probes ever reported for biosensors. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/25618374/Cyclodextrin_functionalized_graphene_gold_nanoparticle_hybrids_with_strong_supramolecular_capability_for_electrochemical_thrombin_aptasensor_ DB - PRIME DP - Unbound Medicine ER -