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Determination of guanine and adenine by high-performance liquid chromatography with a self-fabricated wall-jet/thin-layer electrochemical detector at a glassy carbon electrode.
Talanta. 2015 Mar; 134:354-359.T

Abstract

A sensitive wall-jet/thin-layer amperometric electrochemical detector (ECD) coupled to high-performance liquid chromatography (HPLC) was developed for simultaneous determination of guanine (G) and adenine (A). The analytes were detected at a glassy carbon electrode (GCE) and the HPLC-ECD calibration curves showed good linearity (R(2)>0.997) under optimized conditions. Limits of detection for G and A are 0.6 nM and 1.4 nM (S/N=3), respectively, which are lower than those obtained with an UV-vis detector and a commercial electrochemical detector. We have successfully applied this HPLC-ECD to assess the contents of G and A in hydrochloric acid-digested calf thymus double-stranded DNA. In addition, we compared in detail the analysis of G and A by cyclic voltammetry (CV) and by the HPLC-ECD system on both bare GCE and electroreduced graphene oxide (ERGO) modified GCE. We found that the adsorption of G and A on the electrode surfaces can vary their anodic CV peaks and the competitive adsorption of G and A on the limited sites of the electrode surfaces can cause crosstalk effects on their anodic CV peak signals, but the HPLC-ECD system is insensitive to such electrode-adsorption and can give more reliable analytical results.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Zhou Y
Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China; College of Pharmacy, Zunyi Medical University, Zunyi 563000, China.
Yan H
Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
Xie Q
Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China. Electronic address: xieqj@hunnu.edu.cn.
Yao S
Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.

MeSH

AdenineAdsorptionChromatography, High Pressure LiquidDNAElectrochemical TechniquesElectrodesGraphiteGuanine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25618679

Citation

Zhou, Yaping, et al. "Determination of Guanine and Adenine By High-performance Liquid Chromatography With a Self-fabricated Wall-jet/thin-layer Electrochemical Detector at a Glassy Carbon Electrode." Talanta, vol. 134, 2015, pp. 354-359.
Zhou Y, Yan H, Xie Q, et al. Determination of guanine and adenine by high-performance liquid chromatography with a self-fabricated wall-jet/thin-layer electrochemical detector at a glassy carbon electrode. Talanta. 2015;134:354-359.
Zhou, Y., Yan, H., Xie, Q., & Yao, S. (2015). Determination of guanine and adenine by high-performance liquid chromatography with a self-fabricated wall-jet/thin-layer electrochemical detector at a glassy carbon electrode. Talanta, 134, 354-359. https://doi.org/10.1016/j.talanta.2014.11.042
Zhou Y, et al. Determination of Guanine and Adenine By High-performance Liquid Chromatography With a Self-fabricated Wall-jet/thin-layer Electrochemical Detector at a Glassy Carbon Electrode. Talanta. 2015;134:354-359. PubMed PMID: 25618679.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of guanine and adenine by high-performance liquid chromatography with a self-fabricated wall-jet/thin-layer electrochemical detector at a glassy carbon electrode. AU - Zhou,Yaping, AU - Yan,Hongling, AU - Xie,Qingji, AU - Yao,Shouzhuo, Y1 - 2014/11/29/ PY - 2014/08/30/received PY - 2014/11/19/revised PY - 2014/11/20/accepted PY - 2015/1/26/entrez PY - 2015/1/27/pubmed PY - 2015/10/7/medline KW - Adenine KW - Electrode-adsorption effect KW - Guanine KW - High-performance liquid chromatography KW - Wall-jet/thin-layer amperometric electrochemical detector SP - 354 EP - 359 JF - Talanta JO - Talanta VL - 134 N2 - A sensitive wall-jet/thin-layer amperometric electrochemical detector (ECD) coupled to high-performance liquid chromatography (HPLC) was developed for simultaneous determination of guanine (G) and adenine (A). The analytes were detected at a glassy carbon electrode (GCE) and the HPLC-ECD calibration curves showed good linearity (R(2)>0.997) under optimized conditions. Limits of detection for G and A are 0.6 nM and 1.4 nM (S/N=3), respectively, which are lower than those obtained with an UV-vis detector and a commercial electrochemical detector. We have successfully applied this HPLC-ECD to assess the contents of G and A in hydrochloric acid-digested calf thymus double-stranded DNA. In addition, we compared in detail the analysis of G and A by cyclic voltammetry (CV) and by the HPLC-ECD system on both bare GCE and electroreduced graphene oxide (ERGO) modified GCE. We found that the adsorption of G and A on the electrode surfaces can vary their anodic CV peaks and the competitive adsorption of G and A on the limited sites of the electrode surfaces can cause crosstalk effects on their anodic CV peak signals, but the HPLC-ECD system is insensitive to such electrode-adsorption and can give more reliable analytical results. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/25618679/Determination_of_guanine_and_adenine_by_high_performance_liquid_chromatography_with_a_self_fabricated_wall_jet/thin_layer_electrochemical_detector_at_a_glassy_carbon_electrode_ DB - PRIME DP - Unbound Medicine ER -