Sulphated polysaccharides from Ulva clathrata and Cladosiphon okamuranus seaweeds both inhibit viral attachment/entry and cell-cell fusion, in NDV infection.
Mar Drugs. 2015 Jan 26; 13(2):697-712.MD

Abstract

Sulphated polysaccharides (SP) extracted from seaweeds have antiviral properties and are much less cytotoxic than conventional drugs, but little is known about their mode of action. Combination antiviral chemotherapy may offer advantages over single agent therapy, increasing efficiency, potency and delaying the emergence of resistant virus. The paramyxoviridae family includes pathogens causing morbidity and mortality worldwide in humans and animals, such as the Newcastle Disease Virus (NDV) in poultry. This study aims at determining the antiviral activity and mechanism of action in vitro of an ulvan (SP from the green seaweed Ulva clathrata), and of its mixture with a fucoidan (SP from Cladosiphon okamuranus), against La Sota NDV strain. The ulvan antiviral activity was tested using syncytia formation, exhibiting an IC50 of 0.1 μg/mL; ulvan had a better anti cell-cell spread effect than that previously shown for fucoidan, and inhibited cell-cell fusion via a direct effect on the F0 protein, but did not show any virucidal effect. The mixture of ulvan and fucoidan showed a greater anti-spread effect than SPs alone, but ulvan antagonizes the effect of fucoidan on the viral attachment/entry. Both SPs may be promising antivirals against paramyxovirus infection but their mixture has no clear synergistic advantage.

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Authors+Show Affiliations

Aguilar-Briseño JA

Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Av. Manuel L. Barragán y Av. Pedro de Alba s/n Cd. Universitaria, San Nicolás de los Garza, N.L. 66455, Mexico. albertvirus@gmail.com.

Cruz-Suarez LE

Sassi JF

Centre d'Etude et de Valorisation des Algues, Presqu'île de Pen Lan, 22610 Pleubian, France. Jean-Francois.SASSI@cea.fr.

MeSH

AnimalsAntiviral AgentsBirdsCell FusionCell SurvivalChlorocebus aethiopsDrug Screening Assays, AntitumorHumansNewcastle DiseaseNewcastle disease virusPhaeophytaPlant ExtractsPolysaccharidesSeaweedSpectrophotometry, InfraredVero CellsViral ProteinsVirus Attachment

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25629385

Citation

Aguilar-Briseño, José Alberto, et al. "Sulphated Polysaccharides From Ulva Clathrata and Cladosiphon Okamuranus Seaweeds Both Inhibit Viral Attachment/entry and Cell-cell Fusion, in NDV Infection." Marine Drugs, vol. 13, no. 2, 2015, pp. 697-712.

Aguilar-Briseño JA, Cruz-Suarez LE, Sassi JF, et al. Sulphated polysaccharides from Ulva clathrata and Cladosiphon okamuranus seaweeds both inhibit viral attachment/entry and cell-cell fusion, in NDV infection. Mar Drugs. 2015;13(2):697-712.

Aguilar-Briseño, J. A., Cruz-Suarez, L. E., Sassi, J. F., Ricque-Marie, D., Zapata-Benavides, P., Mendoza-Gamboa, E., Rodríguez-Padilla, C., & Trejo-Avila, L. M. (2015). Sulphated polysaccharides from Ulva clathrata and Cladosiphon okamuranus seaweeds both inhibit viral attachment/entry and cell-cell fusion, in NDV infection. Marine Drugs, 13(2), 697-712. https://doi.org/10.3390/md13020697

Aguilar-Briseño JA, et al. Sulphated Polysaccharides From Ulva Clathrata and Cladosiphon Okamuranus Seaweeds Both Inhibit Viral Attachment/entry and Cell-cell Fusion, in NDV Infection. Mar Drugs. 2015 Jan 26;13(2):697-712. PubMed PMID: 25629385.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Sulphated polysaccharides from Ulva clathrata and Cladosiphon okamuranus seaweeds both inhibit viral attachment/entry and cell-cell fusion, in NDV infection. AU - Aguilar-Briseño,José Alberto, AU - Cruz-Suarez,Lucia Elizabeth, AU - Sassi,Jean-François, AU - Ricque-Marie,Denis, AU - Zapata-Benavides,Pablo, AU - Mendoza-Gamboa,Edgar, AU - Rodríguez-Padilla,Cristina, AU - Trejo-Avila,Laura María, Y1 - 2015/01/26/ PY - 2014/10/31/received PY - 2015/01/06/accepted PY - 2015/1/29/entrez PY - 2015/1/30/pubmed PY - 2016/5/11/medline SP - 697 EP - 712 JF - Marine drugs JO - Mar Drugs VL - 13 IS - 2 N2 - Sulphated polysaccharides (SP) extracted from seaweeds have antiviral properties and are much less cytotoxic than conventional drugs, but little is known about their mode of action. Combination antiviral chemotherapy may offer advantages over single agent therapy, increasing efficiency, potency and delaying the emergence of resistant virus. The paramyxoviridae family includes pathogens causing morbidity and mortality worldwide in humans and animals, such as the Newcastle Disease Virus (NDV) in poultry. This study aims at determining the antiviral activity and mechanism of action in vitro of an ulvan (SP from the green seaweed Ulva clathrata), and of its mixture with a fucoidan (SP from Cladosiphon okamuranus), against La Sota NDV strain. The ulvan antiviral activity was tested using syncytia formation, exhibiting an IC50 of 0.1 μg/mL; ulvan had a better anti cell-cell spread effect than that previously shown for fucoidan, and inhibited cell-cell fusion via a direct effect on the F0 protein, but did not show any virucidal effect. The mixture of ulvan and fucoidan showed a greater anti-spread effect than SPs alone, but ulvan antagonizes the effect of fucoidan on the viral attachment/entry. Both SPs may be promising antivirals against paramyxovirus infection but their mixture has no clear synergistic advantage. SN - 1660-3397 UR - https://www.unboundmedicine.com/medline/citation/25629385/Sulphated_polysaccharides_from_Ulva_clathrata_and_Cladosiphon_okamuranus_seaweeds_both_inhibit_viral_attachment/entry_and_cell_cell_fusion_in_NDV_infection_ DB - PRIME DP - Unbound Medicine ER -

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Sulphated polysaccharides from Ulva clathrata and Cladosiphon okamuranus seaweeds both inhibit viral attachment/entry and cell-cell fusion, in NDV infection.Mar Drugs. 2015 Jan 26; 13(2):697-712.MD