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[Effect of electroacupuncture on CD 34+ endothelial progenitor cell counts in bone marrow and peripheral blood in focal cerebral ischemia/reperfusion rats].
Zhen Ci Yan Jiu. 2014 Dec; 39(6):437-42.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) on CD 34+ endothelial progenitor cells (EPCs) in bone marrow and peripheral blood and the expression of p-AKT protein in bone marrow in focal cerebral ischemia/reperfusion (CI/R) rats, so as to investigate its mechanism underlying improvement of cerebral ischemia.

METHODS

A total of 108 male SD rats were randomly divided into sham operation (sham) group, model (CI/R) group, and EA group which were further divided into 12, 24, 48 h subgroups (n = 12/group, 6 rats for biochemical analysis and the other 6 rats for Western blot analysis). Cl/R model was established by occlusion of the right middle cerebral artery for 2 hours followed by reperfusion. EA (2 Hz/20 Hz) was applied to "Baihui"(GV 20), left "Hegu" (LI 4) and left "Taichong" (LR 3) acupoints for 30 min, once daily. The neurological deficit scores were evaluated using Longa 5-grade standards. Flow cytometer was used to detect the percentages of CD 34+ EPCs in bone marrow and peripheral blood. The expression of p-AKT protein of bone marrow was detected by Western blot.

RESULTS

In comparison with the CIl/R model group, the neurological deficit score were gradually and significantly decreased 48 h after CI/R in the EA group (P<0. 05), suggesting an improvement of the neurological function after EA. Compared with the sham group, the percentages of CD 34+ EPCs in bone marrow and peripheral blood and the expression level of bone-marrow p-AKT protein were significantly up-regulated in the model group at the three time-points after CI/R (P<0. 01, P<0. 05). Following EA intervention, the percentages of CD 34+ EPCs at the three time-points in the peripheral blood, and at time-points of 12 h and 24 h in the bone marrow, and the expression levels of p-AKT protein at the three time-points were significantly further up-regulated in the EA group in comparison with the model group (P<0.05, P<0.01).

CONCLUSION

EA can effectively up-regulate the percentages of CD 34+ EPCs in the bone marrow and peripheral blood, and increase p-AKT protein expression in the bone marrow in CI/R rats, which may contribute to its effect in improving neurological function.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

25632566

Citation

Xie, Chen-chen, et al. "[Effect of Electroacupuncture On CD 34+ Endothelial Progenitor Cell Counts in Bone Marrow and Peripheral Blood in Focal Cerebral Ischemia/reperfusion Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 39, no. 6, 2014, pp. 437-42.
Xie CC, Luo Y, Pang YS, et al. [Effect of electroacupuncture on CD 34+ endothelial progenitor cell counts in bone marrow and peripheral blood in focal cerebral ischemia/reperfusion rats]. Zhen Ci Yan Jiu. 2014;39(6):437-42.
Xie, C. C., Luo, Y., Pang, Y. S., Gao, X., Li, M., Wen, H. Q., & Chen Rui-fang, . (2014). [Effect of electroacupuncture on CD 34+ endothelial progenitor cell counts in bone marrow and peripheral blood in focal cerebral ischemia/reperfusion rats]. Zhen Ci Yan Jiu = Acupuncture Research, 39(6), 437-42.
Xie CC, et al. [Effect of Electroacupuncture On CD 34+ Endothelial Progenitor Cell Counts in Bone Marrow and Peripheral Blood in Focal Cerebral Ischemia/reperfusion Rats]. Zhen Ci Yan Jiu. 2014;39(6):437-42. PubMed PMID: 25632566.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture on CD 34+ endothelial progenitor cell counts in bone marrow and peripheral blood in focal cerebral ischemia/reperfusion rats]. AU - Xie,Chen-chen, AU - Luo,Yong, AU - Pang,Yue-shan, AU - Gao,Xiang, AU - Li,Man, AU - Wen,Hai-qi, AU - Chen Rui-fang,, PY - 2015/1/31/entrez PY - 2015/1/31/pubmed PY - 2015/3/21/medline SP - 437 EP - 42 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 39 IS - 6 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) on CD 34+ endothelial progenitor cells (EPCs) in bone marrow and peripheral blood and the expression of p-AKT protein in bone marrow in focal cerebral ischemia/reperfusion (CI/R) rats, so as to investigate its mechanism underlying improvement of cerebral ischemia. METHODS: A total of 108 male SD rats were randomly divided into sham operation (sham) group, model (CI/R) group, and EA group which were further divided into 12, 24, 48 h subgroups (n = 12/group, 6 rats for biochemical analysis and the other 6 rats for Western blot analysis). Cl/R model was established by occlusion of the right middle cerebral artery for 2 hours followed by reperfusion. EA (2 Hz/20 Hz) was applied to "Baihui"(GV 20), left "Hegu" (LI 4) and left "Taichong" (LR 3) acupoints for 30 min, once daily. The neurological deficit scores were evaluated using Longa 5-grade standards. Flow cytometer was used to detect the percentages of CD 34+ EPCs in bone marrow and peripheral blood. The expression of p-AKT protein of bone marrow was detected by Western blot. RESULTS: In comparison with the CIl/R model group, the neurological deficit score were gradually and significantly decreased 48 h after CI/R in the EA group (P<0. 05), suggesting an improvement of the neurological function after EA. Compared with the sham group, the percentages of CD 34+ EPCs in bone marrow and peripheral blood and the expression level of bone-marrow p-AKT protein were significantly up-regulated in the model group at the three time-points after CI/R (P<0. 01, P<0. 05). Following EA intervention, the percentages of CD 34+ EPCs at the three time-points in the peripheral blood, and at time-points of 12 h and 24 h in the bone marrow, and the expression levels of p-AKT protein at the three time-points were significantly further up-regulated in the EA group in comparison with the model group (P<0.05, P<0.01). CONCLUSION: EA can effectively up-regulate the percentages of CD 34+ EPCs in the bone marrow and peripheral blood, and increase p-AKT protein expression in the bone marrow in CI/R rats, which may contribute to its effect in improving neurological function. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/25632566/[Effect_of_electroacupuncture_on_CD_34+_endothelial_progenitor_cell_counts_in_bone_marrow_and_peripheral_blood_in_focal_cerebral_ischemia/reperfusion_rats]_ DB - PRIME DP - Unbound Medicine ER -