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Effects of infusion of human methemoglobin solution following hydrogen sulfide poisoning.
Clin Toxicol (Phila) 2015; 53(2):93-101CT

Abstract

RATIONALE

We have recently reported that infusion of a solution containing methemoglobin (MetHb) during exposure to hydrogen sulfide results in a rapid and large decrease in the concentration of the pool of soluble/diffusible H2S in the blood. However, since the pool of dissolved H2S disappears very quickly after H2S exposure, it is unclear if the ability of MetHb to "trap" sulfide in the blood has any clinical interest and relevance in the treatment of sulfide poisoning.

METHODS

In anesthetized rats, repetition of short bouts of high level of H2S infusions was applied to allow the rapid development of an oxygen deficit. A solution containing MetHb (600 mg/kg) or its vehicle was administered 1 min and a half after the end of H2S intoxication.

RESULTS

The injection of MetHb solution increased methemoglobinemia to about 6%, almost instantly, but was unable to affect the blood concentration of soluble H2S, which had already vanished at the time of infusion, or to increase combined H2S. In addition, H2S-induced O2 deficit and lactate production as well as the recovery of carotid blood flow and blood pressure were similar in treated and control animals.

CONCLUSION

Our results do not support the view that administration of MetHb or drugs-induced methemoglobinemia during the recovery phase following severe H2S intoxication in sedated rats can restore cellular oxidative metabolism, as the pool of diffusible sulfide, accessible to MetHb, disappears rapidly from the blood after H2S exposure.

Authors+Show Affiliations

Division of Pulmonary and Critical Care Medicine, Department of Medicine, Pennsylvania State University College of Medicine , Hershey, PA , USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25634666

Citation

Chenuel, B, et al. "Effects of Infusion of Human Methemoglobin Solution Following Hydrogen Sulfide Poisoning." Clinical Toxicology (Philadelphia, Pa.), vol. 53, no. 2, 2015, pp. 93-101.
Chenuel B, Sonobe T, Haouzi P. Effects of infusion of human methemoglobin solution following hydrogen sulfide poisoning. Clin Toxicol (Phila). 2015;53(2):93-101.
Chenuel, B., Sonobe, T., & Haouzi, P. (2015). Effects of infusion of human methemoglobin solution following hydrogen sulfide poisoning. Clinical Toxicology (Philadelphia, Pa.), 53(2), pp. 93-101. doi:10.3109/15563650.2014.996570.
Chenuel B, Sonobe T, Haouzi P. Effects of Infusion of Human Methemoglobin Solution Following Hydrogen Sulfide Poisoning. Clin Toxicol (Phila). 2015;53(2):93-101. PubMed PMID: 25634666.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of infusion of human methemoglobin solution following hydrogen sulfide poisoning. AU - Chenuel,B, AU - Sonobe,T, AU - Haouzi,P, Y1 - 2015/01/29/ PY - 2015/1/31/entrez PY - 2015/1/31/pubmed PY - 2015/4/7/medline KW - H2S toxicity KW - Metallo-compound KW - Methemoglobin KW - Rat model SP - 93 EP - 101 JF - Clinical toxicology (Philadelphia, Pa.) JO - Clin Toxicol (Phila) VL - 53 IS - 2 N2 - RATIONALE: We have recently reported that infusion of a solution containing methemoglobin (MetHb) during exposure to hydrogen sulfide results in a rapid and large decrease in the concentration of the pool of soluble/diffusible H2S in the blood. However, since the pool of dissolved H2S disappears very quickly after H2S exposure, it is unclear if the ability of MetHb to "trap" sulfide in the blood has any clinical interest and relevance in the treatment of sulfide poisoning. METHODS: In anesthetized rats, repetition of short bouts of high level of H2S infusions was applied to allow the rapid development of an oxygen deficit. A solution containing MetHb (600 mg/kg) or its vehicle was administered 1 min and a half after the end of H2S intoxication. RESULTS: The injection of MetHb solution increased methemoglobinemia to about 6%, almost instantly, but was unable to affect the blood concentration of soluble H2S, which had already vanished at the time of infusion, or to increase combined H2S. In addition, H2S-induced O2 deficit and lactate production as well as the recovery of carotid blood flow and blood pressure were similar in treated and control animals. CONCLUSION: Our results do not support the view that administration of MetHb or drugs-induced methemoglobinemia during the recovery phase following severe H2S intoxication in sedated rats can restore cellular oxidative metabolism, as the pool of diffusible sulfide, accessible to MetHb, disappears rapidly from the blood after H2S exposure. SN - 1556-9519 UR - https://www.unboundmedicine.com/medline/citation/25634666/Effects_of_infusion_of_human_methemoglobin_solution_following_hydrogen_sulfide_poisoning_ L2 - http://www.tandfonline.com/doi/full/10.3109/15563650.2014.996570 DB - PRIME DP - Unbound Medicine ER -