Tags

Type your tag names separated by a space and hit enter

Adrenergic signaling mediates mechanical hyperalgesia through activation of P2X3 receptors in primary sensory neurons of rats with chronic pancreatitis.
Am J Physiol Gastrointest Liver Physiol. 2015 Apr 15; 308(8):G710-9.AJ

Abstract

The mechanism of pain in chronic pancreatitis (CP) is poorly understood. The aim of this study was designed to investigate roles of norepinephrine (NE) and P2X receptor (P2XR) signaling pathway in the pathogenesis of hyperalgesia in a rat model of CP. CP was induced in male adult rats by intraductal injection of trinitrobenzene sulfonic acid (TNBS). Mechanical hyperalgesia was assessed by referred somatic behaviors to mechanical stimulation of rat abdomen. P2XR-mediated responses of pancreatic dorsal root ganglion (DRG) neurons were measured utilizing calcium imaging and whole cell patch-clamp-recording techniques. Western blot analysis and immunofluorescence were performed to examine protein expression. TNBS injection produced a significant upregulation of P2X3R expression and an increase in ATP-evoked responses of pancreatic DRG neurons. The sensitization of P2X3Rs was reversed by administration of β-adrenergic receptor antagonist propranolol. Incubation of DRG neurons with NE significantly enhanced ATP-induced intracellular calcium signals, which were abolished by propranolol, and partially blocked by protein kinase A inhibitor H-89. Interestingly, TNBS injection led to a significant elevation of NE concentration in DRGs and the pancreas, an upregulation of β2-adrenergic receptor expression in DRGs, and amplification of the NE-induced potentiation of ATP responses. Importantly, pancreatic hyperalgesia was markedly attenuated by administration of purinergic receptor antagonist suramin or A317491 or β2-adrenergic receptor antagonist butoxamine. Sensitization of P2X3Rs, which was likely mediated by adrenergic signaling in primary sensory neurons, contributes to pancreatic pain, thus identifying a potential target for treating pancreatic pain caused by inflammation.

Authors+Show Affiliations

The Affiliated Zhangjiagang Hospital of Soochow University, Suzhou, China;The Affiliated Zhangjiagang Hospital of Soochow University, Suzhou, China; Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Institute of Neuroscience, Soochow University, Suzhou, China;Department of Anesthesiology, Nanjing Jinling Hospital, Nanjing, China.Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Institute of Neuroscience, Soochow University, Suzhou, China;Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Institute of Neuroscience, Soochow University, Suzhou, China;Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Institute of Neuroscience, Soochow University, Suzhou, China;Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Institute of Neuroscience, Soochow University, Suzhou, China;The Affiliated Zhangjiagang Hospital of Soochow University, Suzhou, China; Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Institute of Neuroscience, Soochow University, Suzhou, China; guangyinxu@suda.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25634810

Citation

Wang, Shusheng, et al. "Adrenergic Signaling Mediates Mechanical Hyperalgesia Through Activation of P2X3 Receptors in Primary Sensory Neurons of Rats With Chronic Pancreatitis." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 308, no. 8, 2015, pp. G710-9.
Wang S, Zhu HY, Jin Y, et al. Adrenergic signaling mediates mechanical hyperalgesia through activation of P2X3 receptors in primary sensory neurons of rats with chronic pancreatitis. Am J Physiol Gastrointest Liver Physiol. 2015;308(8):G710-9.
Wang, S., Zhu, H. Y., Jin, Y., Zhou, Y., Hu, S., Liu, T., Jiang, X., & Xu, G. Y. (2015). Adrenergic signaling mediates mechanical hyperalgesia through activation of P2X3 receptors in primary sensory neurons of rats with chronic pancreatitis. American Journal of Physiology. Gastrointestinal and Liver Physiology, 308(8), G710-9. https://doi.org/10.1152/ajpgi.00395.2014
Wang S, et al. Adrenergic Signaling Mediates Mechanical Hyperalgesia Through Activation of P2X3 Receptors in Primary Sensory Neurons of Rats With Chronic Pancreatitis. Am J Physiol Gastrointest Liver Physiol. 2015 Apr 15;308(8):G710-9. PubMed PMID: 25634810.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adrenergic signaling mediates mechanical hyperalgesia through activation of P2X3 receptors in primary sensory neurons of rats with chronic pancreatitis. AU - Wang,Shusheng, AU - Zhu,Hong-Yan, AU - Jin,Yi, AU - Zhou,Youlang, AU - Hu,Shufen, AU - Liu,Tong, AU - Jiang,Xinghong, AU - Xu,Guang-Yin, Y1 - 2015/01/29/ PY - 2014/11/03/received PY - 2015/01/24/accepted PY - 2015/1/31/entrez PY - 2015/1/31/pubmed PY - 2015/6/18/medline KW - chronic pancreatitis KW - dorsal root ganglion KW - norepinephrine KW - protein kinase A KW - purinergic receptors SP - G710 EP - 9 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am J Physiol Gastrointest Liver Physiol VL - 308 IS - 8 N2 - The mechanism of pain in chronic pancreatitis (CP) is poorly understood. The aim of this study was designed to investigate roles of norepinephrine (NE) and P2X receptor (P2XR) signaling pathway in the pathogenesis of hyperalgesia in a rat model of CP. CP was induced in male adult rats by intraductal injection of trinitrobenzene sulfonic acid (TNBS). Mechanical hyperalgesia was assessed by referred somatic behaviors to mechanical stimulation of rat abdomen. P2XR-mediated responses of pancreatic dorsal root ganglion (DRG) neurons were measured utilizing calcium imaging and whole cell patch-clamp-recording techniques. Western blot analysis and immunofluorescence were performed to examine protein expression. TNBS injection produced a significant upregulation of P2X3R expression and an increase in ATP-evoked responses of pancreatic DRG neurons. The sensitization of P2X3Rs was reversed by administration of β-adrenergic receptor antagonist propranolol. Incubation of DRG neurons with NE significantly enhanced ATP-induced intracellular calcium signals, which were abolished by propranolol, and partially blocked by protein kinase A inhibitor H-89. Interestingly, TNBS injection led to a significant elevation of NE concentration in DRGs and the pancreas, an upregulation of β2-adrenergic receptor expression in DRGs, and amplification of the NE-induced potentiation of ATP responses. Importantly, pancreatic hyperalgesia was markedly attenuated by administration of purinergic receptor antagonist suramin or A317491 or β2-adrenergic receptor antagonist butoxamine. Sensitization of P2X3Rs, which was likely mediated by adrenergic signaling in primary sensory neurons, contributes to pancreatic pain, thus identifying a potential target for treating pancreatic pain caused by inflammation. SN - 1522-1547 UR - https://www.unboundmedicine.com/medline/citation/25634810/Adrenergic_signaling_mediates_mechanical_hyperalgesia_through_activation_of_P2X3_receptors_in_primary_sensory_neurons_of_rats_with_chronic_pancreatitis_ L2 - https://journals.physiology.org/doi/10.1152/ajpgi.00395.2014?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -