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Preparation of uniform-sized hemoglobin-albumin microspheres as oxygen carriers by Shirasu porous glass membrane emulsification technique.
Colloids Surf B Biointerfaces. 2015 Mar 01; 127:1-7.CS

Abstract

We have developed a new type of artificial oxygen carrier composed of bovine hemoglobin (bHb) and bovine serum albumin (BSA) prepared by Shirasu porous glass (SPG) membrane emulsification technique. The resultant emulsion droplets containing 10 wt% bHb and 5-20 wt% BSA were subsequently cross-linked by glutaraldehyde to form the microspheres. Due to the uniform pore structure of SPG membranes, the average diameters of bHb10-BSAm microspheres were successfully controlled at around 5 μm with a coefficient of variation of around 10%. In addition, the biocompatibility of the carriers depended on their oxyhemoglobin percentage regardless of their same size. Finally, the P50 values of these microspheres ranged from 8.08 to 11.60 mmHg, which showed a high oxygen affinity and an oxygen delivery function.

Authors+Show Affiliations

Department of Chemical System Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.Department of Chemical System Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.Department of Environmental and Energy Chemistry, Kogakuin University, 2665-1 Nakano-machi, Hachioji-shi, Tokyo 192-0015, Japan.Department of Environmental and Energy Chemistry, Kogakuin University, 2665-1 Nakano-machi, Hachioji-shi, Tokyo 192-0015, Japan.Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.Department of Chemical System Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan; Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: taichi@m.u-tokyo.ac.jp.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25635614

Citation

Lai, Yao-Tong, et al. "Preparation of Uniform-sized Hemoglobin-albumin Microspheres as Oxygen Carriers By Shirasu Porous Glass Membrane Emulsification Technique." Colloids and Surfaces. B, Biointerfaces, vol. 127, 2015, pp. 1-7.
Lai YT, Sato M, Ohta S, et al. Preparation of uniform-sized hemoglobin-albumin microspheres as oxygen carriers by Shirasu porous glass membrane emulsification technique. Colloids Surf B Biointerfaces. 2015;127:1-7.
Lai, Y. T., Sato, M., Ohta, S., Akamatsu, K., Nakao, S., Sakai, Y., & Ito, T. (2015). Preparation of uniform-sized hemoglobin-albumin microspheres as oxygen carriers by Shirasu porous glass membrane emulsification technique. Colloids and Surfaces. B, Biointerfaces, 127, 1-7. https://doi.org/10.1016/j.colsurfb.2015.01.018
Lai YT, et al. Preparation of Uniform-sized Hemoglobin-albumin Microspheres as Oxygen Carriers By Shirasu Porous Glass Membrane Emulsification Technique. Colloids Surf B Biointerfaces. 2015 Mar 1;127:1-7. PubMed PMID: 25635614.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation of uniform-sized hemoglobin-albumin microspheres as oxygen carriers by Shirasu porous glass membrane emulsification technique. AU - Lai,Yao-Tong, AU - Sato,Mayu, AU - Ohta,Seiichi, AU - Akamatsu,Kazuki, AU - Nakao,Shin-ichi, AU - Sakai,Yasuyuki, AU - Ito,Taichi, Y1 - 2015/01/19/ PY - 2014/06/25/received PY - 2014/12/03/revised PY - 2015/01/11/accepted PY - 2015/1/31/entrez PY - 2015/1/31/pubmed PY - 2015/12/22/medline KW - Albumin KW - Hemoglobin KW - Membrane emulsification KW - Oxygen carriers KW - Shirasu porous glass membrane SP - 1 EP - 7 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 127 N2 - We have developed a new type of artificial oxygen carrier composed of bovine hemoglobin (bHb) and bovine serum albumin (BSA) prepared by Shirasu porous glass (SPG) membrane emulsification technique. The resultant emulsion droplets containing 10 wt% bHb and 5-20 wt% BSA were subsequently cross-linked by glutaraldehyde to form the microspheres. Due to the uniform pore structure of SPG membranes, the average diameters of bHb10-BSAm microspheres were successfully controlled at around 5 μm with a coefficient of variation of around 10%. In addition, the biocompatibility of the carriers depended on their oxyhemoglobin percentage regardless of their same size. Finally, the P50 values of these microspheres ranged from 8.08 to 11.60 mmHg, which showed a high oxygen affinity and an oxygen delivery function. SN - 1873-4367 UR - https://www.unboundmedicine.com/medline/citation/25635614/Preparation_of_uniform_sized_hemoglobin_albumin_microspheres_as_oxygen_carriers_by_Shirasu_porous_glass_membrane_emulsification_technique_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0927-7765(15)00025-9 DB - PRIME DP - Unbound Medicine ER -