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Effects of nemorosone, isolated from the plant Clusia rosea, on the cell cycle and gene expression in MCF-7 BUS breast cancer cell lines.

Abstract

BACKGROUND

Breast cancer is the cause of considerable morbidity and mortality in women. While estrogen receptor antagonists have been widely used in breast cancer treatment, patients have increasingly shown resistance to these agents and the identification of novel targeted therapies is therefore required. Nemorosone is the major constituent of the floral resin from Clusia rosea and belongs to the class of polycyclic polyisoprenylated benzophenones of the acylphloroglucinol group. The cytotoxicity of nemorosone in human cancer cell lines has been reported in recent years and has been related to estrogen receptors in breast cancer cells.

METHODS

Changes induced by nemorosone in the cell cycle and gene expression of the MCF-7 BUS (estrogen-dependent) breast cancer cell line were analyzed using flow cytometry and the RT(2) Profiler PCR array, respectively.

RESULTS

In comparison to breast cancer cells without treatment, nemorosone induced discrete cell cycle arrest in the G1 phase and significant depletion in the G2 phase. Moreover, the compound altered the expression of 19 genes related to different pathways, especially the cell cycle, apoptosis and hormone receptors.

CONCLUSION

These promising results justify further studies to clarify mechanisms of action of nemorosone, in view of evaluate the possible use of this benzophenone as adjuvant in the treatment of breast cancer.

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  • Publisher Full Text
  • Authors+Show Affiliations

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    UNESP - Univ. Estadual Paulista, Faculdade de Ciências Farmacêuticas de Araraquara, Departamento de Ciências Biológicas, CEP 14801-902, Araraquara, São Paulo, Brazil.

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    UEL - Univ. Estadual Londrina, Londrina, Paraná, Brazil.

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    UNESP - Univ. Estadual Paulista, Faculdade de Ciências Farmacêuticas de Araraquara, Departamento de Ciências Biológicas, CEP 14801-902, Araraquara, São Paulo, Brazil.

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    UNESP - Univ. Estadual Paulista, Faculdade de Ciências Farmacêuticas de Araraquara, Departamento de Ciências Biológicas, CEP 14801-902, Araraquara, São Paulo, Brazil. Electronic address: flaviabiomed@yahoo.com.br.

    ,

    UNESP - Univ. Estadual Paulista, Faculdade de Ciências Farmacêuticas de Araraquara, Departamento de Ciências Biológicas, CEP 14801-902, Araraquara, São Paulo, Brazil.

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    UNESP - Univ. Estadual Paulista, Faculdade de Ciências Farmacêuticas de Araraquara, Departamento de Ciências Biológicas, CEP 14801-902, Araraquara, São Paulo, Brazil.

    ,

    UNESP - Univ. Estadual Paulista, Faculdade de Ciências Farmacêuticas de Araraquara, Departamento de Ciências Biológicas, CEP 14801-902, Araraquara, São Paulo, Brazil.

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    Instituto de Farmacia y Alimentos (IFAL), Universidad de La Habana, La Habana, Cuba.

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    UNESP - Univ. Estadual Paulista, Instituto de Química, Campus Araraquara, c.p. 355, CEP 14800-900, Araraquara, São Paulo, Brazil.

    UNESP - Univ. Estadual Paulista, Faculdade de Ciências Farmacêuticas de Araraquara, Departamento de Ciências Biológicas, CEP 14801-902, Araraquara, São Paulo, Brazil.

    Source

    MeSH

    Antineoplastic Agents, Phytogenic
    Benzophenones
    Breast Neoplasms
    Cell Cycle
    Clusia
    Female
    Flowers
    Gene Expression Regulation, Neoplastic
    Humans
    MCF-7 Cells

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    25636884

    Citation

    Camargo, M S., et al. "Effects of Nemorosone, Isolated From the Plant Clusia Rosea, On the Cell Cycle and Gene Expression in MCF-7 BUS Breast Cancer Cell Lines." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 22, no. 1, 2015, pp. 153-7.
    Camargo MS, Oliveira MT, Santoni MM, et al. Effects of nemorosone, isolated from the plant Clusia rosea, on the cell cycle and gene expression in MCF-7 BUS breast cancer cell lines. Phytomedicine. 2015;22(1):153-7.
    Camargo, M. S., Oliveira, M. T., Santoni, M. M., Resende, F. A., Oliveira-Höhne, A. P., Espanha, L. G., ... Varanda, E. A. (2015). Effects of nemorosone, isolated from the plant Clusia rosea, on the cell cycle and gene expression in MCF-7 BUS breast cancer cell lines. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 22(1), pp. 153-7. doi:10.1016/j.phymed.2014.11.007.
    Camargo MS, et al. Effects of Nemorosone, Isolated From the Plant Clusia Rosea, On the Cell Cycle and Gene Expression in MCF-7 BUS Breast Cancer Cell Lines. Phytomedicine. 2015 Jan 15;22(1):153-7. PubMed PMID: 25636884.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effects of nemorosone, isolated from the plant Clusia rosea, on the cell cycle and gene expression in MCF-7 BUS breast cancer cell lines. AU - Camargo,M S, AU - Oliveira,M T, AU - Santoni,M M, AU - Resende,F A, AU - Oliveira-Höhne,A P, AU - Espanha,L G, AU - Nogueira,C H, AU - Cuesta-Rubio,O, AU - Vilegas,W, AU - Varanda,E A, Y1 - 2014/11/22/ PY - 2014/03/03/received PY - 2014/09/30/revised PY - 2014/11/15/accepted PY - 2015/2/1/entrez PY - 2015/2/1/pubmed PY - 2015/4/1/medline KW - Breast cancer KW - Cell cycle KW - Clusia rosea KW - Gene expression KW - Nemorosone SP - 153 EP - 7 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 22 IS - 1 N2 - BACKGROUND: Breast cancer is the cause of considerable morbidity and mortality in women. While estrogen receptor antagonists have been widely used in breast cancer treatment, patients have increasingly shown resistance to these agents and the identification of novel targeted therapies is therefore required. Nemorosone is the major constituent of the floral resin from Clusia rosea and belongs to the class of polycyclic polyisoprenylated benzophenones of the acylphloroglucinol group. The cytotoxicity of nemorosone in human cancer cell lines has been reported in recent years and has been related to estrogen receptors in breast cancer cells. METHODS: Changes induced by nemorosone in the cell cycle and gene expression of the MCF-7 BUS (estrogen-dependent) breast cancer cell line were analyzed using flow cytometry and the RT(2) Profiler PCR array, respectively. RESULTS: In comparison to breast cancer cells without treatment, nemorosone induced discrete cell cycle arrest in the G1 phase and significant depletion in the G2 phase. Moreover, the compound altered the expression of 19 genes related to different pathways, especially the cell cycle, apoptosis and hormone receptors. CONCLUSION: These promising results justify further studies to clarify mechanisms of action of nemorosone, in view of evaluate the possible use of this benzophenone as adjuvant in the treatment of breast cancer. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/25636884/Effects_of_nemorosone_isolated_from_the_plant_Clusia_rosea_on_the_cell_cycle_and_gene_expression_in_MCF_7_BUS_breast_cancer_cell_lines_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(14)00388-2 DB - PRIME DP - Unbound Medicine ER -