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High prevalence of CTX-M-15 and first report of CTX-M-3, CTX-M-22, CTX-M-28 and plasmid-mediated AmpC beta-lactamase producing Enterobacteriaceae causing urinary tract infections in Bosnia and Herzegovina in hospital and community settings.
J Infect Chemother. 2015 May; 21(5):363-9.JI

Abstract

AIM

To investigate molecular epidemiology of extended-spectrum β-lactamase/ESBL and plasmid-mediated AmpC β-lactamase/pAmpC producing Gram-negative bacteria causing urinary tract infections (UTIs) in Zenica-Doboj Canton, Bosnia and Herzegovina, in the period Decembar 2009-May 2010.

METHODS

Antibiotic susceptibility was determined by disc diffusion and broth microdilution according to CLSI guidelines. Double-disk synergy test was performed in order to screen for ESBLs/pAmpC beta-lactamases. PCR was used to detect bla(ESBL)/bla(ampC)/bla(carb) genes. Genetic relatedness of the strains was determined by pulsed-field-gel-electrophoresis (PFGE).

RESULTS

Among 85 patients with UTIs caused by ESBL producing isolates, 44 (51.8%) were from in-patients and 41 (48.2%) from outpatients. Klebsiella spp. was the most frequently isolated from in-patients, in 28 (63.6%) cases. Among outpatients, Klebsiella spp./Escherichia coli were the most frequently isolated, in 19 (46.3%)/16 (39.0%) cases. Twenty-one (75.0%) from hospital and nine (47.4%) from outpatient Klebsiella spp. isolates were positive for blaTEM, whereas 27 (96.4%) from in-patients and 6 (31.6%) from outpatient were bla(CTX-M) positive (18 hospital and five outpatient isolates were encoding bla(CTX-M-15)). One Klebsiella oxytoca and one Enterobacter cloacae inpatient isolates were positive for blaCTX-M-28. One Klebsiella pneumoniae outpatient isolate were positive for bla(CTX-M-22) and one E. coli for bla(CTX-M-3). One hospital Proteus mirabilis strain was positive for bla(CMY-2) and two Klebsiella spp. strains for blaDHA-1, whereas two E. coli, one K. oxytoca and one Proteus vulgaris outpatient strains were positive for bla(CMY-2).

CONCLUSION

Identification of bla(CTX-M-3), bla(CTX-M-22) and bla(CTX-M-28) among Enterobacteriaceae is uncommon. In this study we report the emergency of CMY-2 and DHA-1 plasmid-mediated beta-lactamases.

Authors+Show Affiliations

Department for Laboratory Diagnostics, Cantonal Public Health Institute Zenica, fra Ivana Jukića 2, 72 000 Zenica, Bosnia and Herzegovina. Electronic address: ibrahimagic.amir@gmail.com.School of Medicine, University of Zagreb, Šalata 3, 10 000 Zagreb, Croatia; Clinical Department of Clinical and Molecular Microbiology, University Hospital Center Zagreb, Kišpatičeva 12, 10 000 Zagreb, Croatia. Electronic address: branka.bedenic@zg.htnet.hr.Microbiology Department, Biotechnical faculty, University of Ljubljana, Večna pot 111, 1000 Ljubljana, Slovenia. Electronic address: farah_kamb@hotmail.com.Department for Laboratory Diagnostics, Cantonal Public Health Institute Zenica, fra Ivana Jukića 2, 72 000 Zenica, Bosnia and Herzegovina; Faculty for Health Care, University "VITEZ" Travnik, Školska 23, 72 270 Travnik, Bosnia and Herzegovina. Electronic address: selma_kamb@yahoo.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25638292

Citation

Ibrahimagić, Amir, et al. "High Prevalence of CTX-M-15 and First Report of CTX-M-3, CTX-M-22, CTX-M-28 and Plasmid-mediated AmpC Beta-lactamase Producing Enterobacteriaceae Causing Urinary Tract Infections in Bosnia and Herzegovina in Hospital and Community Settings." Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy, vol. 21, no. 5, 2015, pp. 363-9.
Ibrahimagić A, Bedenić B, Kamberović F, et al. High prevalence of CTX-M-15 and first report of CTX-M-3, CTX-M-22, CTX-M-28 and plasmid-mediated AmpC beta-lactamase producing Enterobacteriaceae causing urinary tract infections in Bosnia and Herzegovina in hospital and community settings. J Infect Chemother. 2015;21(5):363-9.
Ibrahimagić, A., Bedenić, B., Kamberović, F., & Uzunović, S. (2015). High prevalence of CTX-M-15 and first report of CTX-M-3, CTX-M-22, CTX-M-28 and plasmid-mediated AmpC beta-lactamase producing Enterobacteriaceae causing urinary tract infections in Bosnia and Herzegovina in hospital and community settings. Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy, 21(5), 363-9. https://doi.org/10.1016/j.jiac.2015.01.003
Ibrahimagić A, et al. High Prevalence of CTX-M-15 and First Report of CTX-M-3, CTX-M-22, CTX-M-28 and Plasmid-mediated AmpC Beta-lactamase Producing Enterobacteriaceae Causing Urinary Tract Infections in Bosnia and Herzegovina in Hospital and Community Settings. J Infect Chemother. 2015;21(5):363-9. PubMed PMID: 25638292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High prevalence of CTX-M-15 and first report of CTX-M-3, CTX-M-22, CTX-M-28 and plasmid-mediated AmpC beta-lactamase producing Enterobacteriaceae causing urinary tract infections in Bosnia and Herzegovina in hospital and community settings. AU - Ibrahimagić,Amir, AU - Bedenić,Branka, AU - Kamberović,Farah, AU - Uzunović,Selma, Y1 - 2015/01/09/ PY - 2014/10/08/received PY - 2014/12/05/revised PY - 2015/01/03/accepted PY - 2015/2/2/entrez PY - 2015/2/2/pubmed PY - 2016/2/27/medline KW - Antibiotic resistance KW - ESBL KW - bla(CMY-2) KW - bla(DHA-1) SP - 363 EP - 9 JF - Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy JO - J. Infect. Chemother. VL - 21 IS - 5 N2 - AIM: To investigate molecular epidemiology of extended-spectrum β-lactamase/ESBL and plasmid-mediated AmpC β-lactamase/pAmpC producing Gram-negative bacteria causing urinary tract infections (UTIs) in Zenica-Doboj Canton, Bosnia and Herzegovina, in the period Decembar 2009-May 2010. METHODS: Antibiotic susceptibility was determined by disc diffusion and broth microdilution according to CLSI guidelines. Double-disk synergy test was performed in order to screen for ESBLs/pAmpC beta-lactamases. PCR was used to detect bla(ESBL)/bla(ampC)/bla(carb) genes. Genetic relatedness of the strains was determined by pulsed-field-gel-electrophoresis (PFGE). RESULTS: Among 85 patients with UTIs caused by ESBL producing isolates, 44 (51.8%) were from in-patients and 41 (48.2%) from outpatients. Klebsiella spp. was the most frequently isolated from in-patients, in 28 (63.6%) cases. Among outpatients, Klebsiella spp./Escherichia coli were the most frequently isolated, in 19 (46.3%)/16 (39.0%) cases. Twenty-one (75.0%) from hospital and nine (47.4%) from outpatient Klebsiella spp. isolates were positive for blaTEM, whereas 27 (96.4%) from in-patients and 6 (31.6%) from outpatient were bla(CTX-M) positive (18 hospital and five outpatient isolates were encoding bla(CTX-M-15)). One Klebsiella oxytoca and one Enterobacter cloacae inpatient isolates were positive for blaCTX-M-28. One Klebsiella pneumoniae outpatient isolate were positive for bla(CTX-M-22) and one E. coli for bla(CTX-M-3). One hospital Proteus mirabilis strain was positive for bla(CMY-2) and two Klebsiella spp. strains for blaDHA-1, whereas two E. coli, one K. oxytoca and one Proteus vulgaris outpatient strains were positive for bla(CMY-2). CONCLUSION: Identification of bla(CTX-M-3), bla(CTX-M-22) and bla(CTX-M-28) among Enterobacteriaceae is uncommon. In this study we report the emergency of CMY-2 and DHA-1 plasmid-mediated beta-lactamases. SN - 1437-7780 UR - https://www.unboundmedicine.com/medline/citation/25638292/High_prevalence_of_CTX_M_15_and_first_report_of_CTX_M_3_CTX_M_22_CTX_M_28_and_plasmid_mediated_AmpC_beta_lactamase_producing_Enterobacteriaceae_causing_urinary_tract_infections_in_Bosnia_and_Herzegovina_in_hospital_and_community_settings_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1341-321X(15)00008-2 DB - PRIME DP - Unbound Medicine ER -