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Lesional infiltration of mast cells, Langerhans cells, T cells and local cytokine profiles in alopecia areata.
Arch Dermatol Res. 2015 May; 307(4):319-31.AD

Abstract

Alopecia areata (AA) is a chronic inflammatory disease mediated by an array of cells and cytokines. Immunohistochemistry (IHC) of histological sections with antibodies to mast cell tryptase, CD4, CD8, CD1a and semi-quantitative real-time PCR analysis of Th1- and Th2-type cytokines were performed in 55 patients to investigate the infiltration features of mast cells (MCs), T lymphocytes and Langerhans cells (LCs) in scalp lesions of patients with AA. In AA patients, increased MCs mainly infiltrated the peri-follicular and peri-vascular areas, and correlated positively with numbers of CD8(+) T lymphocytes in deep peri-follicular areas (P = 0.04), but negatively with CD4(+) T lymphocytes in deep peri-vascular areas (P = 0.031). In patients with active hair loss, LCs in epidermis, deep dermis and peri-vascular were elevated (Ps < 0.05). Infiltration of LCs in upper peri-vascular areas and CD8(+) T cell infiltration in deep peri-follicular areas were positively correlated (R = 0.618, P = 0.011), as well as LCs in deep peri-vascular areas with CD8(+) T cells in upper peri-follicular areas (R = 0.570, P = 0.017). In patients with active hair loss, Th1-type cytokine (IL-2, IL-8, TNF-α) mRNA expression in deep dermis were higher than in upper dermis (Ps < 0.05). However, in patients with non-active hair loss, Th2-type cytokine (IL-5, IL-10) mRNA expression in deep dermis was higher than that in the upper dermis (Ps < 0.05). Positive correlations were found existing between MCs and CD8(+) T cells, as well as between LCs and CD8(+) T cells. In conclusion, findings in this study allow us to propose a close relationship between mast cells and CD8(+) T cells, as well as between LCs and CD8(+) T cells in AA, as well as allergy may interfere with infiltrating T lymphocytes in AA lesional regions. Also, Th1-type cytokine are related to disease activity of alopecia areata, whereas Th2-type cytokines may be associated with persistence of AA.

Authors+Show Affiliations

Department of Dermatology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, Guangdong, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25638328

Citation

Zhang, Xiaoting, et al. "Lesional Infiltration of Mast Cells, Langerhans Cells, T Cells and Local Cytokine Profiles in Alopecia Areata." Archives of Dermatological Research, vol. 307, no. 4, 2015, pp. 319-31.
Zhang X, Zhao Y, Ye Y, et al. Lesional infiltration of mast cells, Langerhans cells, T cells and local cytokine profiles in alopecia areata. Arch Dermatol Res. 2015;307(4):319-31.
Zhang, X., Zhao, Y., Ye, Y., Li, S., Qi, S., Yang, Y., Cao, H., Yang, J., & Zhang, X. (2015). Lesional infiltration of mast cells, Langerhans cells, T cells and local cytokine profiles in alopecia areata. Archives of Dermatological Research, 307(4), 319-31. https://doi.org/10.1007/s00403-015-1539-1
Zhang X, et al. Lesional Infiltration of Mast Cells, Langerhans Cells, T Cells and Local Cytokine Profiles in Alopecia Areata. Arch Dermatol Res. 2015;307(4):319-31. PubMed PMID: 25638328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lesional infiltration of mast cells, Langerhans cells, T cells and local cytokine profiles in alopecia areata. AU - Zhang,Xiaoting, AU - Zhao,Ying, AU - Ye,Yanting, AU - Li,Shuifeng, AU - Qi,Shiling, AU - Yang,Yuqing, AU - Cao,Hui, AU - Yang,Jian, AU - Zhang,Xingqi, Y1 - 2015/02/01/ PY - 2014/05/19/received PY - 2015/01/07/accepted PY - 2014/12/21/revised PY - 2015/2/2/entrez PY - 2015/2/2/pubmed PY - 2016/2/10/medline SP - 319 EP - 31 JF - Archives of dermatological research JO - Arch. Dermatol. Res. VL - 307 IS - 4 N2 - Alopecia areata (AA) is a chronic inflammatory disease mediated by an array of cells and cytokines. Immunohistochemistry (IHC) of histological sections with antibodies to mast cell tryptase, CD4, CD8, CD1a and semi-quantitative real-time PCR analysis of Th1- and Th2-type cytokines were performed in 55 patients to investigate the infiltration features of mast cells (MCs), T lymphocytes and Langerhans cells (LCs) in scalp lesions of patients with AA. In AA patients, increased MCs mainly infiltrated the peri-follicular and peri-vascular areas, and correlated positively with numbers of CD8(+) T lymphocytes in deep peri-follicular areas (P = 0.04), but negatively with CD4(+) T lymphocytes in deep peri-vascular areas (P = 0.031). In patients with active hair loss, LCs in epidermis, deep dermis and peri-vascular were elevated (Ps < 0.05). Infiltration of LCs in upper peri-vascular areas and CD8(+) T cell infiltration in deep peri-follicular areas were positively correlated (R = 0.618, P = 0.011), as well as LCs in deep peri-vascular areas with CD8(+) T cells in upper peri-follicular areas (R = 0.570, P = 0.017). In patients with active hair loss, Th1-type cytokine (IL-2, IL-8, TNF-α) mRNA expression in deep dermis were higher than in upper dermis (Ps < 0.05). However, in patients with non-active hair loss, Th2-type cytokine (IL-5, IL-10) mRNA expression in deep dermis was higher than that in the upper dermis (Ps < 0.05). Positive correlations were found existing between MCs and CD8(+) T cells, as well as between LCs and CD8(+) T cells. In conclusion, findings in this study allow us to propose a close relationship between mast cells and CD8(+) T cells, as well as between LCs and CD8(+) T cells in AA, as well as allergy may interfere with infiltrating T lymphocytes in AA lesional regions. Also, Th1-type cytokine are related to disease activity of alopecia areata, whereas Th2-type cytokines may be associated with persistence of AA. SN - 1432-069X UR - https://www.unboundmedicine.com/medline/citation/25638328/Lesional_infiltration_of_mast_cells_Langerhans_cells_T_cells_and_local_cytokine_profiles_in_alopecia_areata_ L2 - https://link.springer.com/article/10.1007/s00403-015-1539-1 DB - PRIME DP - Unbound Medicine ER -