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Clinical and genetic correlations of familial Hirschsprung's disease.
J Pediatr Surg. 2015 Feb; 50(2):285-8.JP

Abstract

BACKGROUND

The risk of familial transmission in Hirschsprung's disease (HSCR) currently lacks correlation between the clinical phenotype and the underlying genetic factors. The aim of this study was to clinically evaluate familial HSCR transmission and to correlate with the genetic background.

METHODS

Clinical and gene analysis of familial HSCR patients were explored. DNA from 45 patients (35 kindreds) was screened for genetic variations of the RET, and EDNRB genes were screened for genetic variation by semi-automated bi-directional sequencing analysis and matched to controls.

MAIN RESULTS

Male:female ratio (3:1) had a female proband in 4 families. Aganglionosis was significantly more frequent with total colonic aganglionosis (TCA) in 40% familial cases (viz: 17/43 (43%) vs. 19/342 non-familial patients (5.6%) (p<0.01)). Transmission of S-HSCR was observed in 13 (31%), which was associated with EDNRB variation. RET gene promoter variation correlated with extended aganglionosis in 6/35 kindreds (17%). In 3 kindreds, both significant EDNRB and RET mutations were identified and where present were associated with increased penetrance in succeeding generations. An increased penetrance with succeeding generations occurred in 6 (14%). In a further 3 generation family, extensive variations in exon 6, 13, and 18 affected 3 males with progressive penetration and aganglionic length, including total intestinal aganglionosis in the further offspring. RET and MEN association was noted in 5 kindreds (14.3%) related to RET variations at Cysteine sites.

CONCLUSIONS

Cumulative effects of the RET and EDNRB genes contribute to long-segment and total colonic aganglionosis.

Authors+Show Affiliations

Division of Paediatric Surgery, University of Stellenbosch, Tygerberg, Western Cape, South Africa. Electronic address: swm@sun.ac.za.Division of Paediatric Surgery, University of Stellenbosch, Tygerberg, Western Cape, South Africa.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25638620

Citation

Moore, Sam W., and Monique Zaahl. "Clinical and Genetic Correlations of Familial Hirschsprung's Disease." Journal of Pediatric Surgery, vol. 50, no. 2, 2015, pp. 285-8.
Moore SW, Zaahl M. Clinical and genetic correlations of familial Hirschsprung's disease. J Pediatr Surg. 2015;50(2):285-8.
Moore, S. W., & Zaahl, M. (2015). Clinical and genetic correlations of familial Hirschsprung's disease. Journal of Pediatric Surgery, 50(2), 285-8. https://doi.org/10.1016/j.jpedsurg.2014.11.016
Moore SW, Zaahl M. Clinical and Genetic Correlations of Familial Hirschsprung's Disease. J Pediatr Surg. 2015;50(2):285-8. PubMed PMID: 25638620.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clinical and genetic correlations of familial Hirschsprung's disease. AU - Moore,Sam W, AU - Zaahl,Monique, Y1 - 2014/11/07/ PY - 2014/10/25/received PY - 2014/11/02/accepted PY - 2015/2/2/entrez PY - 2015/2/2/pubmed PY - 2016/1/13/medline KW - EDNRB gene KW - Familial KW - Genetic RET protooncogene KW - Hirschsprung’s disease SP - 285 EP - 8 JF - Journal of pediatric surgery JO - J Pediatr Surg VL - 50 IS - 2 N2 - BACKGROUND: The risk of familial transmission in Hirschsprung's disease (HSCR) currently lacks correlation between the clinical phenotype and the underlying genetic factors. The aim of this study was to clinically evaluate familial HSCR transmission and to correlate with the genetic background. METHODS: Clinical and gene analysis of familial HSCR patients were explored. DNA from 45 patients (35 kindreds) was screened for genetic variations of the RET, and EDNRB genes were screened for genetic variation by semi-automated bi-directional sequencing analysis and matched to controls. MAIN RESULTS: Male:female ratio (3:1) had a female proband in 4 families. Aganglionosis was significantly more frequent with total colonic aganglionosis (TCA) in 40% familial cases (viz: 17/43 (43%) vs. 19/342 non-familial patients (5.6%) (p<0.01)). Transmission of S-HSCR was observed in 13 (31%), which was associated with EDNRB variation. RET gene promoter variation correlated with extended aganglionosis in 6/35 kindreds (17%). In 3 kindreds, both significant EDNRB and RET mutations were identified and where present were associated with increased penetrance in succeeding generations. An increased penetrance with succeeding generations occurred in 6 (14%). In a further 3 generation family, extensive variations in exon 6, 13, and 18 affected 3 males with progressive penetration and aganglionic length, including total intestinal aganglionosis in the further offspring. RET and MEN association was noted in 5 kindreds (14.3%) related to RET variations at Cysteine sites. CONCLUSIONS: Cumulative effects of the RET and EDNRB genes contribute to long-segment and total colonic aganglionosis. SN - 1531-5037 UR - https://www.unboundmedicine.com/medline/citation/25638620/Clinical_and_genetic_correlations_of_familial_Hirschsprung's_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-3468(14)00726-X DB - PRIME DP - Unbound Medicine ER -