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Effect of curcumin on hepatic antioxidant enzymes activities and gene expressions in rats intoxicated with aflatoxin B1.

Abstract

Twenty-eight rats were examined in a 5-week experiment to investigate the effect of curcumin on gene expression and activities of hepatic antioxidant enzymes in rats intoxicated with aflatoxin B1 (AFB1 ). The rats were divided into four groups. Rats in 1-4 groups served as control, oral curcumin treated (15 mg/kg body weight), single i.p. dose of AFB1 (3 mg/kg body weight) and combination of single i.p. dose of AFB1 with oral curcumin treated, respectively. AFB1 Liver damage and oxidative stress were evident in untreated AFB1 -intoxicated rats as indicated by a significant elevation in hepatic transaminases, elevation in lipid peroxide biomarkers (thiobarbituric acid reactive substances; TBARS), reduction of reduced glutathione (GSH) concentration, reduction in the activities of antioxidant enzymes namely catalase (CAT), total superoxide dismutase (SOD), glutathione peroxidase (GPX) and glutathione-S-transferase (GST) and down-regulation of gene expression of these antioxidant enzymes compared to control. Liver sections of rats intoxicated with AFB1 showed a disrupted lobular architecture, scattered necrotic cells and biliary proliferation. Administration of curcumin with AFB1 resulted in amelioration of AFB1 -induced effects compared to untreated AFB1 -intoxicated rats via an up-regulation of antioxidant enzyme gene expression, activation of the expressed genes and increase in the availability of GSH.

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  • Publisher Full Text
  • Authors+Show Affiliations

    Department of Physiology, Biochemistry and Pharmacology (Biochemistry), College of Veterinary Medicine and Animal Resources, King Faisal University, P.O. Box 400, Al-Hufof, 31982, Saudi Arabia; Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Egypt.

    Source

    Phytotherapy research : PTR 29:1 2015 Jan pg 134-40

    MeSH

    Aflatoxin B1
    Animals
    Antioxidants
    Catalase
    Curcumin
    Gene Expression
    Glutathione
    Glutathione Peroxidase
    Glutathione Transferase
    Lipid Peroxidation
    Lipid Peroxides
    Liver
    Male
    Oxidation-Reduction
    Oxidative Stress
    Rats
    Superoxide Dismutase
    Thiobarbituric Acid Reactive Substances

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    25639897

    Citation

    TY - JOUR T1 - Effect of curcumin on hepatic antioxidant enzymes activities and gene expressions in rats intoxicated with aflatoxin B1. A1 - El-Bahr,S M, Y1 - 2014/10/08/ PY - 2014/7/20/received PY - 2014/9/4/revised PY - 2014/9/10/accepted PY - 2014/10/8/aheadofprint PY - 2015/2/3/entrez PY - 2015/2/3/pubmed PY - 2015/3/31/medline KW - Curcuma longa KW - antioxidants KW - biomarkers KW - liver damage KW - oxidative stress SP - 134 EP - 40 JF - Phytotherapy research : PTR JO - Phytother Res VL - 29 IS - 1 N2 - Twenty-eight rats were examined in a 5-week experiment to investigate the effect of curcumin on gene expression and activities of hepatic antioxidant enzymes in rats intoxicated with aflatoxin B1 (AFB1 ). The rats were divided into four groups. Rats in 1-4 groups served as control, oral curcumin treated (15 mg/kg body weight), single i.p. dose of AFB1 (3 mg/kg body weight) and combination of single i.p. dose of AFB1 with oral curcumin treated, respectively. AFB1 Liver damage and oxidative stress were evident in untreated AFB1 -intoxicated rats as indicated by a significant elevation in hepatic transaminases, elevation in lipid peroxide biomarkers (thiobarbituric acid reactive substances; TBARS), reduction of reduced glutathione (GSH) concentration, reduction in the activities of antioxidant enzymes namely catalase (CAT), total superoxide dismutase (SOD), glutathione peroxidase (GPX) and glutathione-S-transferase (GST) and down-regulation of gene expression of these antioxidant enzymes compared to control. Liver sections of rats intoxicated with AFB1 showed a disrupted lobular architecture, scattered necrotic cells and biliary proliferation. Administration of curcumin with AFB1 resulted in amelioration of AFB1 -induced effects compared to untreated AFB1 -intoxicated rats via an up-regulation of antioxidant enzyme gene expression, activation of the expressed genes and increase in the availability of GSH. SN - 1099-1573 UR - https://www.unboundmedicine.com/medline/citation/25639897/Effect_of_curcumin_on_hepatic_antioxidant_enzymes_activities_and_gene_expressions_in_rats_intoxicated_with_aflatoxin_B1_ L2 - http://dx.doi.org/10.1002/ptr.5239 ER -