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Genetic engineering of the Xa10 promoter for broad-spectrum and durable resistance to Xanthomonas oryzae pv. oryzae.
Plant Biotechnol J. 2015 Sep; 13(7):993-1001.PB

Abstract

Many pathovars of plant pathogenic bacteria Xanthomonas species inject transcription activator-like (TAL) effectors into plant host cells to promote disease susceptibility or trigger disease resistance. The rice TAL effector-dependent disease resistance gene Xa10 confers narrow-spectrum race-specific resistance to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight disease in rice. To generate broad-spectrum and durable resistance to Xoo, we developed a modified Xa10 gene, designated as Xa10(E5) . Xa10(E5) has an EBE-amended promoter containing 5 tandemly arranged EBEs each responding specifically to a corresponding virulent or avirulent TAL effector and a stable transgenic rice line containing Xa10(E5) was generated in the cultivar Nipponbare. The Xa10(E5) gene was specifically induced by Xoo strains that harbour the corresponding TAL effectors and conferred TAL effector-dependent resistance to the pathogens at all developmental stages of rice. Further disease evaluation demonstrated that the Xa10(E5) gene in either Nipponbare or 9311 genetic backgrounds provided broad-spectrum disease resistance to 27 of the 28 Xoo strains collected from 11 countries. The development of Xa10(E5) and transgenic rice lines provides new genetic materials for molecular breeding of rice for broad-spectrum and durable disease resistance to bacterial blight.

Authors+Show Affiliations

Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore. Department of Biological Sciences, National University of Singapore, Singapore, Singapore.Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore. Rice Research Institute, Anhui Academy of Agricultural Sciences, Anhui, China.Department of Plant Pathology, Kansas State University, Manhattan, KS, USA.Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore. Department of Biological Sciences, National University of Singapore, Singapore, Singapore. School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25644581

Citation

Zeng, Xuan, et al. "Genetic Engineering of the Xa10 Promoter for Broad-spectrum and Durable Resistance to Xanthomonas Oryzae Pv. Oryzae." Plant Biotechnology Journal, vol. 13, no. 7, 2015, pp. 993-1001.
Zeng X, Tian D, Gu K, et al. Genetic engineering of the Xa10 promoter for broad-spectrum and durable resistance to Xanthomonas oryzae pv. oryzae. Plant Biotechnol J. 2015;13(7):993-1001.
Zeng, X., Tian, D., Gu, K., Zhou, Z., Yang, X., Luo, Y., White, F. F., & Yin, Z. (2015). Genetic engineering of the Xa10 promoter for broad-spectrum and durable resistance to Xanthomonas oryzae pv. oryzae. Plant Biotechnology Journal, 13(7), 993-1001. https://doi.org/10.1111/pbi.12342
Zeng X, et al. Genetic Engineering of the Xa10 Promoter for Broad-spectrum and Durable Resistance to Xanthomonas Oryzae Pv. Oryzae. Plant Biotechnol J. 2015;13(7):993-1001. PubMed PMID: 25644581.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic engineering of the Xa10 promoter for broad-spectrum and durable resistance to Xanthomonas oryzae pv. oryzae. AU - Zeng,Xuan, AU - Tian,Dongsheng, AU - Gu,Keyu, AU - Zhou,Zhiyun, AU - Yang,Xiaobei, AU - Luo,Yanchang, AU - White,Frank F, AU - Yin,Zhongchao, Y1 - 2015/02/03/ PY - 2014/10/01/received PY - 2014/10/09/revised PY - 2014/10/19/accepted PY - 2015/2/4/entrez PY - 2015/2/4/pubmed PY - 2016/5/20/medline KW - R gene KW - Rice KW - TAL effector KW - Xa10 KW - Xanthomonas oryzae pv. oryzae KW - bacterial blight SP - 993 EP - 1001 JF - Plant biotechnology journal JO - Plant Biotechnol J VL - 13 IS - 7 N2 - Many pathovars of plant pathogenic bacteria Xanthomonas species inject transcription activator-like (TAL) effectors into plant host cells to promote disease susceptibility or trigger disease resistance. The rice TAL effector-dependent disease resistance gene Xa10 confers narrow-spectrum race-specific resistance to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight disease in rice. To generate broad-spectrum and durable resistance to Xoo, we developed a modified Xa10 gene, designated as Xa10(E5) . Xa10(E5) has an EBE-amended promoter containing 5 tandemly arranged EBEs each responding specifically to a corresponding virulent or avirulent TAL effector and a stable transgenic rice line containing Xa10(E5) was generated in the cultivar Nipponbare. The Xa10(E5) gene was specifically induced by Xoo strains that harbour the corresponding TAL effectors and conferred TAL effector-dependent resistance to the pathogens at all developmental stages of rice. Further disease evaluation demonstrated that the Xa10(E5) gene in either Nipponbare or 9311 genetic backgrounds provided broad-spectrum disease resistance to 27 of the 28 Xoo strains collected from 11 countries. The development of Xa10(E5) and transgenic rice lines provides new genetic materials for molecular breeding of rice for broad-spectrum and durable disease resistance to bacterial blight. SN - 1467-7652 UR - https://www.unboundmedicine.com/medline/citation/25644581/Genetic_engineering_of_the_Xa10_promoter_for_broad_spectrum_and_durable_resistance_to_Xanthomonas_oryzae_pv__oryzae_ L2 - https://doi.org/10.1111/pbi.12342 DB - PRIME DP - Unbound Medicine ER -