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Standardized extract of Vitex doniana Sweet stalls protein oxidation, lipid peroxidation and DNA fragmention in acetaminophen-induced hepatotoxicity.
J Ethnopharmacol. 2015 Apr 22; 164:273-82.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Vitex doniana fruits are locally used in Nigeria as a remedy in the treatment of jaundice and liver related disease. The effect of methanolic extract of Vitex doniana fruits on acetaminophen induced protein oxidation, lipid peroxidation and DNA fragmentation was investigated in mice.

MATERIALS AND METHODS

Antioxidant activity of the extract (0.2-1.0mg/mL) was investigated in vitro using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide ion, hydrogen peroxide, hydroxyl radical and ferric ion reducing system. Vitex doniana extract at 1.0mg/mL scavenged DPPH, superoxide ion, hydrogen peroxide, and hydroxyl radical by 86%, 78%, 80% and 72% respectively, it also reduced ferric ion significantly. Hepatoprotective effect of Vitex doniana fruits was monitored in acetaminophen-induced hepatotoxicity in mice.

RESULTS

Acetaminophen-mediated alterations in serum alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, albumin and total bilirubin levels in mice were significantly (P<0.05) attenuated by the extract. Similarly, acetaminophen-mediated decrease in activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose 6-phosphate dehydrogenase was significantly (P<0.05) attenuated in the liver of mice. Increased levels of conjugated dienes, lipid hydroperoxides, malondialdehyde, protein carbonyl and fragmented DNA were significantly (P<0.05) lowered by methanolic extract of Vitex doniana fruits.

CONCLUSIONS

Overall, the results of this study show that Vitex doniana fruits possess antioxidant properties and halted acetaminophen-mediated oxidative rout on cellular proteins, lipids and DNA, made possible by β-sitosterol, platycodin D, apigenin, saikosaponin, chrysin and ellagitanin in the extract.

Authors+Show Affiliations

Antioxidants, Free Radicals, Functional Foods and Toxicology Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria. Electronic address: ajiboyeyong@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25645189

Citation

Ajiboye, T O.. "Standardized Extract of Vitex Doniana Sweet Stalls Protein Oxidation, Lipid Peroxidation and DNA Fragmention in Acetaminophen-induced Hepatotoxicity." Journal of Ethnopharmacology, vol. 164, 2015, pp. 273-82.
Ajiboye TO. Standardized extract of Vitex doniana Sweet stalls protein oxidation, lipid peroxidation and DNA fragmention in acetaminophen-induced hepatotoxicity. J Ethnopharmacol. 2015;164:273-82.
Ajiboye, T. O. (2015). Standardized extract of Vitex doniana Sweet stalls protein oxidation, lipid peroxidation and DNA fragmention in acetaminophen-induced hepatotoxicity. Journal of Ethnopharmacology, 164, 273-82. https://doi.org/10.1016/j.jep.2015.01.026
Ajiboye TO. Standardized Extract of Vitex Doniana Sweet Stalls Protein Oxidation, Lipid Peroxidation and DNA Fragmention in Acetaminophen-induced Hepatotoxicity. J Ethnopharmacol. 2015 Apr 22;164:273-82. PubMed PMID: 25645189.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Standardized extract of Vitex doniana Sweet stalls protein oxidation, lipid peroxidation and DNA fragmention in acetaminophen-induced hepatotoxicity. A1 - Ajiboye,T O, Y1 - 2015/01/30/ PY - 2014/10/02/received PY - 2015/01/14/revised PY - 2015/01/21/accepted PY - 2015/2/4/entrez PY - 2015/2/4/pubmed PY - 2016/1/13/medline KW - Acetaminophen KW - Acetaminophen (PUBChem CID: 1983) KW - Fragmented DNA KW - Lipid peroxidation KW - Oxidative stress KW - Protein oxidation KW - Vitex doniana fruits SP - 273 EP - 82 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 164 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Vitex doniana fruits are locally used in Nigeria as a remedy in the treatment of jaundice and liver related disease. The effect of methanolic extract of Vitex doniana fruits on acetaminophen induced protein oxidation, lipid peroxidation and DNA fragmentation was investigated in mice. MATERIALS AND METHODS: Antioxidant activity of the extract (0.2-1.0mg/mL) was investigated in vitro using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide ion, hydrogen peroxide, hydroxyl radical and ferric ion reducing system. Vitex doniana extract at 1.0mg/mL scavenged DPPH, superoxide ion, hydrogen peroxide, and hydroxyl radical by 86%, 78%, 80% and 72% respectively, it also reduced ferric ion significantly. Hepatoprotective effect of Vitex doniana fruits was monitored in acetaminophen-induced hepatotoxicity in mice. RESULTS: Acetaminophen-mediated alterations in serum alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, albumin and total bilirubin levels in mice were significantly (P<0.05) attenuated by the extract. Similarly, acetaminophen-mediated decrease in activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose 6-phosphate dehydrogenase was significantly (P<0.05) attenuated in the liver of mice. Increased levels of conjugated dienes, lipid hydroperoxides, malondialdehyde, protein carbonyl and fragmented DNA were significantly (P<0.05) lowered by methanolic extract of Vitex doniana fruits. CONCLUSIONS: Overall, the results of this study show that Vitex doniana fruits possess antioxidant properties and halted acetaminophen-mediated oxidative rout on cellular proteins, lipids and DNA, made possible by β-sitosterol, platycodin D, apigenin, saikosaponin, chrysin and ellagitanin in the extract. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/25645189/Standardized_extract_of_Vitex_doniana_Sweet_stalls_protein_oxidation_lipid_peroxidation_and_DNA_fragmention_in_acetaminophen_induced_hepatotoxicity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(15)00041-0 DB - PRIME DP - Unbound Medicine ER -