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Simultaneous determination of seven taxoids in rat plasma by UPLC-MS/MS and pharmacokinetic study after oral administration of Taxus yunnanensis extracts.
J Pharm Biomed Anal. 2015 Mar 25; 107:346-54.JP

Abstract

A rapid, sensitive and reliable method has been developed and validated for the simultaneous determination of seven taxoids including 10-deacetylbaccatin III (10-DAB III), baccatin III, 5-epi-canadensene, taxinine M, 10-deacetyltaxol (10-DAT), cephalomannine and paclitaxel in rat plasma using docetaxel as the internal standard (IS). The plasma samples were pretreated by liquid-liquid extraction with methyl tert-butyl ether. The chromatographic separation was achieved on a C18 column (50 mm × 2.1 mm, 1.8 μm, Waters, USA) with a gradient elution program consisting of methanol and water (containing 0.1% formic acid) at a flow rate of 0.2 mL/min. Detection was performed under the selected reaction monitoring (SRM) scan using an electrospray ionization (ESI) in the positive ion mode. The mass transitions were as follows: m/z 567.4→444.9 for 10-DAB III, m/z 609.0→549.3 for baccatin III, m/z 617.4→496.9 for 5-epi-canadensene, m/z 709.6→649.3 for taxinine M, m/z 834.8→307.9 for 10-DAT, m/z 854.5→285.4 for cephalomannine, m/z 876.8→307.3 for paclitaxel and m/z 830.8→549.6 for IS, respectively. All calibration curves exhibited good linearity (r(2)>0.99) over a wide concentration range for all components. The intra-day and inter-day precisions at three different levels were both less than 14.3% in terms of relative standard deviation (RSD) and the accuracies ranged from -8.3% to 14.8% in terms of relative error (RE). The extraction recoveries of the seven compounds ranged from 62.5% to 100.5%. The developed method was successfully applied to the pharmacokinetic study of the seven taxoids in rat plasma after oral administration of the crude extract of the twigs and leaves of Taxus yunnanensis.

Authors+Show Affiliations

School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China.School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China.School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China.School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China.School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China.School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China.School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China. Electronic address: jinjing@mail.sysu.edu.cn.School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China. Electronic address: huangmin@mail.sysu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25645339

Citation

Liu, Bin, et al. "Simultaneous Determination of Seven Taxoids in Rat Plasma By UPLC-MS/MS and Pharmacokinetic Study After Oral Administration of Taxus Yunnanensis Extracts." Journal of Pharmaceutical and Biomedical Analysis, vol. 107, 2015, pp. 346-54.
Liu B, Gou X, Bai X, et al. Simultaneous determination of seven taxoids in rat plasma by UPLC-MS/MS and pharmacokinetic study after oral administration of Taxus yunnanensis extracts. J Pharm Biomed Anal. 2015;107:346-54.
Liu, B., Gou, X., Bai, X., Hou, X., Li, D., Zhong, G., Jin, J., & Huang, M. (2015). Simultaneous determination of seven taxoids in rat plasma by UPLC-MS/MS and pharmacokinetic study after oral administration of Taxus yunnanensis extracts. Journal of Pharmaceutical and Biomedical Analysis, 107, 346-54. https://doi.org/10.1016/j.jpba.2015.01.001
Liu B, et al. Simultaneous Determination of Seven Taxoids in Rat Plasma By UPLC-MS/MS and Pharmacokinetic Study After Oral Administration of Taxus Yunnanensis Extracts. J Pharm Biomed Anal. 2015 Mar 25;107:346-54. PubMed PMID: 25645339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of seven taxoids in rat plasma by UPLC-MS/MS and pharmacokinetic study after oral administration of Taxus yunnanensis extracts. AU - Liu,Bin, AU - Gou,Xiaoli, AU - Bai,Xupeng, AU - Hou,Xiangyu, AU - Li,Dongshun, AU - Zhong,Guoping, AU - Jin,Jing, AU - Huang,Min, Y1 - 2015/01/10/ PY - 2014/10/06/received PY - 2015/01/01/accepted PY - 2015/2/4/entrez PY - 2015/2/4/pubmed PY - 2015/12/15/medline KW - Pharmacokinetics KW - Plasma concentration KW - Taxoids KW - Taxus yunnanensis KW - UPLC–MS/MS SP - 346 EP - 54 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 107 N2 - A rapid, sensitive and reliable method has been developed and validated for the simultaneous determination of seven taxoids including 10-deacetylbaccatin III (10-DAB III), baccatin III, 5-epi-canadensene, taxinine M, 10-deacetyltaxol (10-DAT), cephalomannine and paclitaxel in rat plasma using docetaxel as the internal standard (IS). The plasma samples were pretreated by liquid-liquid extraction with methyl tert-butyl ether. The chromatographic separation was achieved on a C18 column (50 mm × 2.1 mm, 1.8 μm, Waters, USA) with a gradient elution program consisting of methanol and water (containing 0.1% formic acid) at a flow rate of 0.2 mL/min. Detection was performed under the selected reaction monitoring (SRM) scan using an electrospray ionization (ESI) in the positive ion mode. The mass transitions were as follows: m/z 567.4→444.9 for 10-DAB III, m/z 609.0→549.3 for baccatin III, m/z 617.4→496.9 for 5-epi-canadensene, m/z 709.6→649.3 for taxinine M, m/z 834.8→307.9 for 10-DAT, m/z 854.5→285.4 for cephalomannine, m/z 876.8→307.3 for paclitaxel and m/z 830.8→549.6 for IS, respectively. All calibration curves exhibited good linearity (r(2)>0.99) over a wide concentration range for all components. The intra-day and inter-day precisions at three different levels were both less than 14.3% in terms of relative standard deviation (RSD) and the accuracies ranged from -8.3% to 14.8% in terms of relative error (RE). The extraction recoveries of the seven compounds ranged from 62.5% to 100.5%. The developed method was successfully applied to the pharmacokinetic study of the seven taxoids in rat plasma after oral administration of the crude extract of the twigs and leaves of Taxus yunnanensis. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/25645339/Simultaneous_determination_of_seven_taxoids_in_rat_plasma_by_UPLC_MS/MS_and_pharmacokinetic_study_after_oral_administration_of_Taxus_yunnanensis_extracts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(15)00002-3 DB - PRIME DP - Unbound Medicine ER -