Tags

Type your tag names separated by a space and hit enter

Vitamin B12 supplementation during pregnancy and postpartum improves B12 status of both mothers and infants but vaccine response in mothers only: a randomized clinical trial in Bangladesh.
Eur J Nutr. 2016 Feb; 55(1):281-93.EJ

Abstract

PURPOSE

Poor vitamin B12 (B12) status is associated with adverse outcomes in pregnancy and infancy. Little is known about effects of B12 supplementation on immune function. The present study aimed to evaluate effects of pre- and postnatal B12 supplementation on biomarkers of B12 status and vaccine-specific responses in mothers and infants.

METHOD

In a blinded, placebo-controlled trial, Bangladeshi women (n = 68, age 18-35 years, hemoglobin <110 g/L, 11-14 weeks pregnant) were randomized to receive 250 μg/day B12 or a placebo throughout pregnancy and 3-month postpartum along with 60 mg iron + 400 μg folate. Women were immunized with pandemic influenza A (H1N1) vaccine at 26- to 28-week gestation. Blood from mothers (baseline, 72-h post-delivery, 3-month postpartum), newborns and infants (3-month) was analyzed for hemoglobin, B12, methylmalonic acid (MMA), total homocysteine (tHcy), ferritin and serum transferrin receptor, C-reactive protein (CRP) and alpha-1-acid glycoprotein (AGP). Vitamin B12 was also assessed in breast milk. H1N1-specific antibodies were determined in plasma and colostrum/breast milk.

RESULTS

At baseline, 26% women were B12 deficient (<150 pmol/L), 40% had marginal status (150-220 pmol/L), 43% had elevated MMA (>271 nmol/L), and 31% had elevated tHcy (>10 μmol/L). Supplementation increased B12 in plasma, colostrums and breast milk (p < 0.05) and lowered MMA in neonates, mothers and infants at 3 months (p < 0.05). B12 supplementation significantly increased H1N1-specific IgA responses in plasma and colostrums in mothers and reduced proportion of infants with elevated AGP and CRP compared with placebo.

CONCLUSION

Supplementation with 250 μg/day B12 during pregnancy and lactation substantially improved maternal, infant and breast milk B12 status. Maternal supplementation improved H1N1 vaccine-specific responses in mothers only and may alleviate inflammatory responses in infants.

Authors+Show Affiliations

Nutritional Biochemistry Laboratory, International Centre for Diarrheal Disease Research, Bangladesh (ICDDR,B), 68 Shaheed Tajuddin Ahmed Sarani, Mohakhali, Dhaka, 1212, Bangladesh. Department of Nutrition, University of California, Davis, CA, USA.Nutritional Biochemistry Laboratory, International Centre for Diarrheal Disease Research, Bangladesh (ICDDR,B), 68 Shaheed Tajuddin Ahmed Sarani, Mohakhali, Dhaka, 1212, Bangladesh.Nutritional Biochemistry Laboratory, International Centre for Diarrheal Disease Research, Bangladesh (ICDDR,B), 68 Shaheed Tajuddin Ahmed Sarani, Mohakhali, Dhaka, 1212, Bangladesh.Nutritional Biochemistry Laboratory, International Centre for Diarrheal Disease Research, Bangladesh (ICDDR,B), 68 Shaheed Tajuddin Ahmed Sarani, Mohakhali, Dhaka, 1212, Bangladesh.Nutritional Biochemistry Laboratory, International Centre for Diarrheal Disease Research, Bangladesh (ICDDR,B), 68 Shaheed Tajuddin Ahmed Sarani, Mohakhali, Dhaka, 1212, Bangladesh.Nutritional Biochemistry Laboratory, International Centre for Diarrheal Disease Research, Bangladesh (ICDDR,B), 68 Shaheed Tajuddin Ahmed Sarani, Mohakhali, Dhaka, 1212, Bangladesh.ARS Western Human Nutrition Research Center, USDA, Davis, CA, USA.ARS Western Human Nutrition Research Center, USDA, Davis, CA, USA. Department of Nutrition, University of California, Davis, CA, USA.Nutritional Biochemistry Laboratory, International Centre for Diarrheal Disease Research, Bangladesh (ICDDR,B), 68 Shaheed Tajuddin Ahmed Sarani, Mohakhali, Dhaka, 1212, Bangladesh.ARS Western Human Nutrition Research Center, USDA, Davis, CA, USA. Department of Nutrition, University of California, Davis, CA, USA.Nutritional Biochemistry Laboratory, International Centre for Diarrheal Disease Research, Bangladesh (ICDDR,B), 68 Shaheed Tajuddin Ahmed Sarani, Mohakhali, Dhaka, 1212, Bangladesh. rubhana@icddrb.org.

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25648738

Citation

Siddiqua, Towfida J., et al. "Vitamin B12 Supplementation During Pregnancy and Postpartum Improves B12 Status of Both Mothers and Infants but Vaccine Response in Mothers Only: a Randomized Clinical Trial in Bangladesh." European Journal of Nutrition, vol. 55, no. 1, 2016, pp. 281-93.
Siddiqua TJ, Ahmad SM, Ahsan KB, et al. Vitamin B12 supplementation during pregnancy and postpartum improves B12 status of both mothers and infants but vaccine response in mothers only: a randomized clinical trial in Bangladesh. Eur J Nutr. 2016;55(1):281-93.
Siddiqua, T. J., Ahmad, S. M., Ahsan, K. B., Rashid, M., Roy, A., Rahman, S. M., Shahab-Ferdows, S., Hampel, D., Ahmed, T., Allen, L. H., & Raqib, R. (2016). Vitamin B12 supplementation during pregnancy and postpartum improves B12 status of both mothers and infants but vaccine response in mothers only: a randomized clinical trial in Bangladesh. European Journal of Nutrition, 55(1), 281-93. https://doi.org/10.1007/s00394-015-0845-x
Siddiqua TJ, et al. Vitamin B12 Supplementation During Pregnancy and Postpartum Improves B12 Status of Both Mothers and Infants but Vaccine Response in Mothers Only: a Randomized Clinical Trial in Bangladesh. Eur J Nutr. 2016;55(1):281-93. PubMed PMID: 25648738.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vitamin B12 supplementation during pregnancy and postpartum improves B12 status of both mothers and infants but vaccine response in mothers only: a randomized clinical trial in Bangladesh. AU - Siddiqua,Towfida J, AU - Ahmad,Shaikh M, AU - Ahsan,Khalid B, AU - Rashid,Mamunur, AU - Roy,Anjan, AU - Rahman,Syed M, AU - Shahab-Ferdows,Setareh, AU - Hampel,Daniela, AU - Ahmed,Tahmeed, AU - Allen,Lindsay H, AU - Raqib,Rubhana, Y1 - 2015/02/04/ PY - 2014/04/28/received PY - 2015/01/26/accepted PY - 2015/2/5/entrez PY - 2015/2/5/pubmed PY - 2016/11/9/medline KW - Breast milk B12 KW - Methylmalonic acid KW - Vaccine response KW - Vitamin B12 supplementation SP - 281 EP - 93 JF - European journal of nutrition JO - Eur J Nutr VL - 55 IS - 1 N2 - PURPOSE: Poor vitamin B12 (B12) status is associated with adverse outcomes in pregnancy and infancy. Little is known about effects of B12 supplementation on immune function. The present study aimed to evaluate effects of pre- and postnatal B12 supplementation on biomarkers of B12 status and vaccine-specific responses in mothers and infants. METHOD: In a blinded, placebo-controlled trial, Bangladeshi women (n = 68, age 18-35 years, hemoglobin <110 g/L, 11-14 weeks pregnant) were randomized to receive 250 μg/day B12 or a placebo throughout pregnancy and 3-month postpartum along with 60 mg iron + 400 μg folate. Women were immunized with pandemic influenza A (H1N1) vaccine at 26- to 28-week gestation. Blood from mothers (baseline, 72-h post-delivery, 3-month postpartum), newborns and infants (3-month) was analyzed for hemoglobin, B12, methylmalonic acid (MMA), total homocysteine (tHcy), ferritin and serum transferrin receptor, C-reactive protein (CRP) and alpha-1-acid glycoprotein (AGP). Vitamin B12 was also assessed in breast milk. H1N1-specific antibodies were determined in plasma and colostrum/breast milk. RESULTS: At baseline, 26% women were B12 deficient (<150 pmol/L), 40% had marginal status (150-220 pmol/L), 43% had elevated MMA (>271 nmol/L), and 31% had elevated tHcy (>10 μmol/L). Supplementation increased B12 in plasma, colostrums and breast milk (p < 0.05) and lowered MMA in neonates, mothers and infants at 3 months (p < 0.05). B12 supplementation significantly increased H1N1-specific IgA responses in plasma and colostrums in mothers and reduced proportion of infants with elevated AGP and CRP compared with placebo. CONCLUSION: Supplementation with 250 μg/day B12 during pregnancy and lactation substantially improved maternal, infant and breast milk B12 status. Maternal supplementation improved H1N1 vaccine-specific responses in mothers only and may alleviate inflammatory responses in infants. SN - 1436-6215 UR - https://www.unboundmedicine.com/medline/citation/25648738/Vitamin_B12_supplementation_during_pregnancy_and_postpartum_improves_B12_status_of_both_mothers_and_infants_but_vaccine_response_in_mothers_only:_a_randomized_clinical_trial_in_Bangladesh_ L2 - https://dx.doi.org/10.1007/s00394-015-0845-x DB - PRIME DP - Unbound Medicine ER -