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Evaluation of antioxidant, total phenol and flavonoid content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts.
Appl Biochem Biotechnol. 2015 Apr; 175(7):3231-43.AB

Abstract

Artocarpus altilis (breadfruit) pulp, peel and whole fruit were extracted with various solvents such as hexane, dichloromethane (DCM) and methanol. The antioxidant activity of these extracts were examined using the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test. IC50 was 55 ± 5.89 μg/ml for the pulp part of methanol extract. In the β-carotene bleaching assay, the antioxidant activity was 90.02 ± 1.51 % for the positive control (Trolox) and 88.34 ± 1.31 % for the pulp part of the fruit methanol extract. The total phenolic content of the crude extracts was determined using the Folin-Ciocalteu procedure; methanol pulp part demonstrated the highest phenol content value of 781 ± 52.97 mg GAE/g of dry sample. While the total flavonoid content was determined using the aluminium chloride colorimetric assay, the highest value of 6213.33 ± 142.22 mg QE/g was indicated by pulp part of the fruit methanol extract. The antimicrobial activity of the crude extracts was tested using disc diffusion method against pathogenic microorganisms: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus, Salmonella typhimurium, Escherichia coli, Klebsiella pneumonia and Candida albicans. Methanol extract of pulp part was recorded to have the highest zone of inhibition against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentration (MIC) and MBC/minimal fungicidal concentration (MFC) for the extracts were also determined using the microdilution method ranging from 4000 to 63 μg/ml against pathogenic microbes. The MBC/MFC values varied from 250 to 4000 μg/ml. A correlation between antioxidant activity assays, antimicrobial activity and phenolic content was established. The results shows that the various parts of A. altilis fruit extracts promising antioxidant activities have potential bioactivities due to high content of phenolic compounds.

Authors+Show Affiliations

Department of Biomedical Science, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia (IIUM), Jalaln Sultan Ahmad Shah, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25649443

Citation

Jalal, Tara Kamal, et al. "Evaluation of Antioxidant, Total Phenol and Flavonoid Content and Antimicrobial Activities of Artocarpus Altilis (breadfruit) of Underutilized Tropical Fruit Extracts." Applied Biochemistry and Biotechnology, vol. 175, no. 7, 2015, pp. 3231-43.
Jalal TK, Ahmed IA, Mikail M, et al. Evaluation of antioxidant, total phenol and flavonoid content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts. Appl Biochem Biotechnol. 2015;175(7):3231-43.
Jalal, T. K., Ahmed, I. A., Mikail, M., Momand, L., Draman, S., Isa, M. L., Abdull Rasad, M. S., Nor Omar, M., Ibrahim, M., & Abdul Wahab, R. (2015). Evaluation of antioxidant, total phenol and flavonoid content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts. Applied Biochemistry and Biotechnology, 175(7), 3231-43. https://doi.org/10.1007/s12010-015-1499-0
Jalal TK, et al. Evaluation of Antioxidant, Total Phenol and Flavonoid Content and Antimicrobial Activities of Artocarpus Altilis (breadfruit) of Underutilized Tropical Fruit Extracts. Appl Biochem Biotechnol. 2015;175(7):3231-43. PubMed PMID: 25649443.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of antioxidant, total phenol and flavonoid content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts. AU - Jalal,Tara Kamal, AU - Ahmed,Idris Adewale, AU - Mikail,Maryam, AU - Momand,Lailuma, AU - Draman,Samsul, AU - Isa,Muhammad Lokman Md, AU - Abdull Rasad,Mohammad Syaiful Bahari, AU - Nor Omar,Muhammad, AU - Ibrahim,Muhammad, AU - Abdul Wahab,Ridhwan, Y1 - 2015/02/04/ PY - 2014/08/03/received PY - 2015/01/19/accepted PY - 2015/2/5/entrez PY - 2015/2/5/pubmed PY - 2015/12/17/medline SP - 3231 EP - 43 JF - Applied biochemistry and biotechnology JO - Appl Biochem Biotechnol VL - 175 IS - 7 N2 - Artocarpus altilis (breadfruit) pulp, peel and whole fruit were extracted with various solvents such as hexane, dichloromethane (DCM) and methanol. The antioxidant activity of these extracts were examined using the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test. IC50 was 55 ± 5.89 μg/ml for the pulp part of methanol extract. In the β-carotene bleaching assay, the antioxidant activity was 90.02 ± 1.51 % for the positive control (Trolox) and 88.34 ± 1.31 % for the pulp part of the fruit methanol extract. The total phenolic content of the crude extracts was determined using the Folin-Ciocalteu procedure; methanol pulp part demonstrated the highest phenol content value of 781 ± 52.97 mg GAE/g of dry sample. While the total flavonoid content was determined using the aluminium chloride colorimetric assay, the highest value of 6213.33 ± 142.22 mg QE/g was indicated by pulp part of the fruit methanol extract. The antimicrobial activity of the crude extracts was tested using disc diffusion method against pathogenic microorganisms: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus, Salmonella typhimurium, Escherichia coli, Klebsiella pneumonia and Candida albicans. Methanol extract of pulp part was recorded to have the highest zone of inhibition against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentration (MIC) and MBC/minimal fungicidal concentration (MFC) for the extracts were also determined using the microdilution method ranging from 4000 to 63 μg/ml against pathogenic microbes. The MBC/MFC values varied from 250 to 4000 μg/ml. A correlation between antioxidant activity assays, antimicrobial activity and phenolic content was established. The results shows that the various parts of A. altilis fruit extracts promising antioxidant activities have potential bioactivities due to high content of phenolic compounds. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/25649443/Evaluation_of_antioxidant_total_phenol_and_flavonoid_content_and_antimicrobial_activities_of_Artocarpus_altilis__breadfruit__of_underutilized_tropical_fruit_extracts_ L2 - https://dx.doi.org/10.1007/s12010-015-1499-0 DB - PRIME DP - Unbound Medicine ER -