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An HPLC method to determine sennoside A and sennoside B in Sennae fructus and Sennae folium.
Pharmeur Bio Sci Notes 2014; 2014:92-102PB

Abstract

INTRODUCTION

The current Ph. Eur. monographs for senna pods, senna leaf and senna leaf dry extract standardised describe a photometric assay based on the Bornträger reaction to determine hydroxyanthracene glycosides, calculated as sennoside B. The method is timeconsuming, unspecific for sennosides and the precision is not adequate for a modern assay.

AIM

The photometric method shall therefore be replaced by a modern HPLC method. About 70 % of the total anthrachinone content in herbal drugs of senna species is due to sennoside A and sennoside B. These substances are therefore suitable for the standardisation of Senna products. The Japanese Pharmacopoeia (JP) already describes an HPLC method to determine sennoside A and sennoside B in the monograph for senna leaf. It uses ion-pair chromatography with tetraheptylammoniumbromide. The procedure described in the monograph has a runtime of 70 min.

METHOD

The adapted and validated method described here uses solid-phase extraction (SPE) which allows a selective sample preparation by using an anion exchange phase. A conventional RP C18 column Tosh TSKgel ODS-80TS (4.6 mm × 150 mm), 5 μm, was used as stationary phase and acetonitrile for chromatography R, water R, phosphoric acid R (200:800:1 V/V/V) as mobile phase. The flow rate was 1.2 mL/min, the column temperature 40 °C, the detection wavelength 380 nm, and the injection volume 20 μL. The runtime is 10 min, the chromatogram shows 2 peaks due to sennoside A/B and 2 additional smaller compounds. One of them is rhein-8-O-glucoside.

RESULTS

The procedure has been successfully validated according to ICH guidelines. We analysed 6 batches of Senna. The pods (Senna angustifolia) showed a total content of sennoside A and B of 1.74-2.76 % m/m and the content of senna leaves was clearly lower with 1.07-1.19 % m/m, respectively.

CONCLUSION

The suggested method is considered to be suitable to determine sennoside A and sennoside B in senna leaves and senna pods. The consideration is based on the performed validation and on the results for the analysed samples. A short run time and better resolution are clear advantages of the suggested method, compared to other methods.

Authors+Show Affiliations

Zurich University of Applied Science, Institute of Biotechnology, Research Group of Phytopharmacy, Einsiedlerstrasse 31, 8820 Wädenswil, Switzerland, immanuel.rosenthal@gmail.com.Zurich University of Applied Science, Institute of Biotechnology, Research Group of Phytopharmacy, Einsiedlerstrasse 31, 8820 Wädenswil, Switzerland, wola@zhaw.ch.Zurich University of Applied Science, Institute of Biotechnology, Research Group of Phytopharmacy, Einsiedlerstrasse 31, 8820 Wädenswil, Switzerland, mier@zhaw.ch.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25655246

Citation

Rosenthal, Immanuel, et al. "An HPLC Method to Determine Sennoside a and Sennoside B in Sennae Fructus and Sennae Folium." Pharmeuropa Bio & Scientific Notes, vol. 2014, 2014, pp. 92-102.
Rosenthal I, Wolfram E, Meier B. An HPLC method to determine sennoside A and sennoside B in Sennae fructus and Sennae folium. Pharmeur Bio Sci Notes. 2014;2014:92-102.
Rosenthal, I., Wolfram, E., & Meier, B. (2014). An HPLC method to determine sennoside A and sennoside B in Sennae fructus and Sennae folium. Pharmeuropa Bio & Scientific Notes, 2014, pp. 92-102.
Rosenthal I, Wolfram E, Meier B. An HPLC Method to Determine Sennoside a and Sennoside B in Sennae Fructus and Sennae Folium. Pharmeur Bio Sci Notes. 2014;2014:92-102. PubMed PMID: 25655246.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An HPLC method to determine sennoside A and sennoside B in Sennae fructus and Sennae folium. AU - Rosenthal,Immanuel, AU - Wolfram,Evelyn, AU - Meier,Beat, PY - 2015/2/7/entrez PY - 2015/2/7/pubmed PY - 2015/10/20/medline KW - HPLC KW - senna leaf KW - senna pods KW - sennoside A KW - sennoside B KW - validation SP - 92 EP - 102 JF - Pharmeuropa bio & scientific notes JO - Pharmeur Bio Sci Notes VL - 2014 N2 - INTRODUCTION: The current Ph. Eur. monographs for senna pods, senna leaf and senna leaf dry extract standardised describe a photometric assay based on the Bornträger reaction to determine hydroxyanthracene glycosides, calculated as sennoside B. The method is timeconsuming, unspecific for sennosides and the precision is not adequate for a modern assay. AIM: The photometric method shall therefore be replaced by a modern HPLC method. About 70 % of the total anthrachinone content in herbal drugs of senna species is due to sennoside A and sennoside B. These substances are therefore suitable for the standardisation of Senna products. The Japanese Pharmacopoeia (JP) already describes an HPLC method to determine sennoside A and sennoside B in the monograph for senna leaf. It uses ion-pair chromatography with tetraheptylammoniumbromide. The procedure described in the monograph has a runtime of 70 min. METHOD: The adapted and validated method described here uses solid-phase extraction (SPE) which allows a selective sample preparation by using an anion exchange phase. A conventional RP C18 column Tosh TSKgel ODS-80TS (4.6 mm × 150 mm), 5 μm, was used as stationary phase and acetonitrile for chromatography R, water R, phosphoric acid R (200:800:1 V/V/V) as mobile phase. The flow rate was 1.2 mL/min, the column temperature 40 °C, the detection wavelength 380 nm, and the injection volume 20 μL. The runtime is 10 min, the chromatogram shows 2 peaks due to sennoside A/B and 2 additional smaller compounds. One of them is rhein-8-O-glucoside. RESULTS: The procedure has been successfully validated according to ICH guidelines. We analysed 6 batches of Senna. The pods (Senna angustifolia) showed a total content of sennoside A and B of 1.74-2.76 % m/m and the content of senna leaves was clearly lower with 1.07-1.19 % m/m, respectively. CONCLUSION: The suggested method is considered to be suitable to determine sennoside A and sennoside B in senna leaves and senna pods. The consideration is based on the performed validation and on the results for the analysed samples. A short run time and better resolution are clear advantages of the suggested method, compared to other methods. SN - 2075-2504 UR - https://www.unboundmedicine.com/medline/citation/25655246/An_HPLC_method_to_determine_sennoside A_and_sennoside B_in_Sennae_fructus_and_Sennae_folium_ DB - PRIME DP - Unbound Medicine ER -