TY - JOUR T1 - Structural characterization and anticoagulant activity of a sulfated polysaccharide from the green alga Codium divaricatum. AU - Li,Na, AU - Mao,Wenjun, AU - Yan,Mengxia, AU - Liu,Xue, AU - Xia,Zheng, AU - Wang,Shuyao, AU - Xiao,Bo, AU - Chen,Chenglong, AU - Zhang,Lifang, AU - Cao,Sujian, Y1 - 2015/01/02/ PY - 2014/09/03/received PY - 2014/12/04/revised PY - 2014/12/05/accepted PY - 2015/2/10/entrez PY - 2015/2/11/pubmed PY - 2015/11/18/medline KW - Anticoagulant activity KW - Codium divaricatum KW - Marine green alga KW - Structural characterization KW - Sulfated polysaccharide SP - 175 EP - 82 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 121 N2 - A sulfated polysaccharide, designated CP2-1, was isolated from the green alga Codium divaricatum by water extraction and purified by anion-exchange and size-exclusion chromatography. CP2-1 is a galactan which is highly sulfated and substituted with pyruvic acid ketals. On the basis of chemical and spectroscopic analyses, the backbone of CP2-1 was mainly composed of (1→3)-β-d-galactopyranose residues, branched by single (1→)-β-d-galactopyranose units attached to the main chain at C-4 positions. The degree of branching was estimated to be about 12.2%. Sulfate groups were at C-4 of (1→3)-β-d-galactopyranose and C-6 of non-reducing terminal galactose residues. In addition, the ketals of pyruvic acid were found at 3,4- of non-reducing terminal galactose residues forming a five-membered ring. CP2-1 possessed a high anticoagulant activity as assessed by the activated partial thromboplastin time and thrombin time assays. The investigation demonstrated that CP2-1 was an anticoagulant-active sulfated polysaccharide distinguishing from other sulfated polysaccharides from marine green algae. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/25659687/Structural_characterization_and_anticoagulant_activity_of_a_sulfated_polysaccharide_from_the_green_alga_Codium_divaricatum_ DB - PRIME DP - Unbound Medicine ER -