TY - JOUR T1 - Intrathecal L-arginine reduces the antinociception of sevoflurane in formalin-induced pain in rats. AU - Chen,Jian-Qing, AU - Zeng,Yin-Ming, AU - Dai,Ti-Jun, AU - Tang,Qi-Feng, Y1 - 2015/02/07/ PY - 2014/10/03/received PY - 2015/02/01/revised PY - 2015/02/03/accepted PY - 2015/2/10/entrez PY - 2015/2/11/pubmed PY - 2015/9/15/medline KW - Antinociception KW - Nitric oxide KW - Pain KW - Sevoflurane KW - c-Fos KW - l-arginine SP - 156 EP - 60 JF - Neuroscience letters JO - Neurosci Lett VL - 590 N2 - The purpose of this study was to investigate the contribution of spinal nitric oxide (NO) to the antinociceptive effects of emulsified sevoflurane in rats. Formalin tests were used to assess the nociceptive response. Immunohistochemistry was performed to determine the effects of emulsified sevoflurane on formalin-induced changes of Fos-like immunoreactive (Fos-LI)-positive neurons in the spinal cord. We found that emulsified sevoflurane administered intraperitoneally at a dosage of 2.5 ml/kg did not impair motor performance in rats, but it significantly decreased the formalin-induced paw licking time. Furthermore, Fos-LI-positive neurons were mainly found in the ipsilateral dorsal horn after the injection of formalin. The administration of emulsified sevoflurane significantly decreased Fos-LI-labeled neurons. Finally, intrathecal L-arginine alone did not affect the basal pain threshold, but it significantly decreased the antinociceptive response of emulsified sevoflurane against formalin injection and the suppressive effects of sevoflurane on formalin-induced Fos protein expression (P<0.05). These data suggest that spinal cord NO is involved in the antinociception of sevoflurane in rats. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/25660619/Intrathecal_L_arginine_reduces_the_antinociception_of_sevoflurane_in_formalin_induced_pain_in_rats_ DB - PRIME DP - Unbound Medicine ER -