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Rapid determination of ruscogenin in rat plasma with application to pharmacokinetic study.

Abstract

A sensitive and rapid ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine ruscogenin in rat plasma using midazolam as the internal standard (IS). Sample preparation was accomplished through a liquid-liquid extraction procedure with ethyl acetate to 0.2mL plasma sample. The analyte and IS were separated on an Acquity UPLC BEH C18 column (2.1mm×50mm, 1.7μm) with the mobile phase of acetonitrile and 1% formic acid in water with gradient elution at a flow rate of 0.40mL/min. Ruscogenin and IS were eluted at 1.74 and 1.11min, respectively. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with positive-ion electrospray ionization (ESI) by multiple reactions monitoring (MRM) of the transitions at m/z 431.2→287.0 for ruscogenin and m/z 326.2→291.1 for IS. The linearity of this method was found to be within the concentration range of 2-1000ng/mL with a lower limit of quantification of 2ng/mL. Only 2.0min was needed for an analytical run. The matrix effect was 92.4-107.3% for ruscogenin. The intra- and inter-day precision (RSD%) were less than 11.2% and accuracy (RE%) was within ±9.8%. The recovery ranged from 75.4% to 86.3%. Ruscogenin was sufficiently stable under all relevant analytical conditions. The method was also successfully applied to the pharmacokinetic study of ruscogenin in rats.

Authors+Show Affiliations

Department of Pharmacy, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, China.Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.Department of Pharmacy, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, China.Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. Electronic address: shwurong@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25660717

Citation

Ji, Pei-ying, et al. "Rapid Determination of Ruscogenin in Rat Plasma With Application to Pharmacokinetic Study." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 985, 2015, pp. 71-4.
Ji PY, Li ZW, Yang Q, et al. Rapid determination of ruscogenin in rat plasma with application to pharmacokinetic study. J Chromatogr B Analyt Technol Biomed Life Sci. 2015;985:71-4.
Ji, P. Y., Li, Z. W., Yang, Q., & Wu, R. (2015). Rapid determination of ruscogenin in rat plasma with application to pharmacokinetic study. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 985, 71-4. https://doi.org/10.1016/j.jchromb.2015.01.022
Ji PY, et al. Rapid Determination of Ruscogenin in Rat Plasma With Application to Pharmacokinetic Study. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Mar 15;985:71-4. PubMed PMID: 25660717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid determination of ruscogenin in rat plasma with application to pharmacokinetic study. AU - Ji,Pei-ying, AU - Li,Zhi-wen, AU - Yang,Qing, AU - Wu,Rong, Y1 - 2015/01/23/ PY - 2014/11/21/received PY - 2015/01/13/revised PY - 2015/01/16/accepted PY - 2015/2/10/entrez PY - 2015/2/11/pubmed PY - 2015/11/18/medline KW - Pharmacokinetics KW - Rat plasma KW - Ruscogenin KW - UPLC-MS/MS SP - 71 EP - 4 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 985 N2 - A sensitive and rapid ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine ruscogenin in rat plasma using midazolam as the internal standard (IS). Sample preparation was accomplished through a liquid-liquid extraction procedure with ethyl acetate to 0.2mL plasma sample. The analyte and IS were separated on an Acquity UPLC BEH C18 column (2.1mm×50mm, 1.7μm) with the mobile phase of acetonitrile and 1% formic acid in water with gradient elution at a flow rate of 0.40mL/min. Ruscogenin and IS were eluted at 1.74 and 1.11min, respectively. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with positive-ion electrospray ionization (ESI) by multiple reactions monitoring (MRM) of the transitions at m/z 431.2→287.0 for ruscogenin and m/z 326.2→291.1 for IS. The linearity of this method was found to be within the concentration range of 2-1000ng/mL with a lower limit of quantification of 2ng/mL. Only 2.0min was needed for an analytical run. The matrix effect was 92.4-107.3% for ruscogenin. The intra- and inter-day precision (RSD%) were less than 11.2% and accuracy (RE%) was within ±9.8%. The recovery ranged from 75.4% to 86.3%. Ruscogenin was sufficiently stable under all relevant analytical conditions. The method was also successfully applied to the pharmacokinetic study of ruscogenin in rats. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/25660717/Rapid_determination_of_ruscogenin_in_rat_plasma_with_application_to_pharmacokinetic_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(15)00055-0 DB - PRIME DP - Unbound Medicine ER -