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Adaptive regulation of digestive serine proteases in the larval midgut of Helicoverpa armigera in response to a plant protease inhibitor.
Insect Biochem Mol Biol. 2015 Apr; 59:18-29.IB

Abstract

Protease inhibitors (PIs) are direct defenses induced by plants in response to herbivory. PIs reduce herbivore digestive efficiency by inhibiting insects' digestive proteases; in turn insects can adapt to PIs by generally increasing protease levels and/or by inducing the expression of PI-insensitive proteases. Helicoverpa armigera, a highly polyphagous lepidopteran insect pest, is known for its ability to adapt to PIs. To advance our molecular and functional understanding of the regulation of digestive proteases, we performed a comprehensive gene expression experiment of H. armigera exposed to soybean Kunitz trypsin inhibitor (SKTI) using a custom-designed microarray. We observed poor larval growth on the SKTI diet until 24 h, however after 48 h larvae attained comparable weight to that of control diet. Although initially the expression of several trypsins and chymotrypsins increased, eventually the expression of some trypsins decreased, while the number of chymotrypsins and their expression increased in response to SKTI. Some of the diverged serine proteases were also differentially expressed. The expression of serine proteases observed using microarrays were further validated by qRT-PCR at different time points (12, 24, 48, 72 and 96 h) after the start of SKTI ingestion. There were also large changes in transcriptional patterns over time in the control diet. Carbohydrate metabolism and immune defense genes were affected in response to SKTI ingestion. Enzyme assays revealed reduced trypsin-specific activity and increased chymotrypsin-specific activity in response to SKTI. The differential regulation of trypsins and chymotrypsins at the transcript and protein levels accompanying a rebound in growth rate indicates that induction of SKTI-insensitive proteases is an effective strategy of H. armigera in coping with this protease inhibitor in its diet.

Authors+Show Affiliations

Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straβe 8, D-07745 Jena, Germany.Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straβe 8, D-07745 Jena, Germany.Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straβe 8, D-07745 Jena, Germany.Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straβe 8, D-07745 Jena, Germany. Electronic address: heckel@ice.mpg.de.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25662099

Citation

Kuwar, Suyog S., et al. "Adaptive Regulation of Digestive Serine Proteases in the Larval Midgut of Helicoverpa Armigera in Response to a Plant Protease Inhibitor." Insect Biochemistry and Molecular Biology, vol. 59, 2015, pp. 18-29.
Kuwar SS, Pauchet Y, Vogel H, et al. Adaptive regulation of digestive serine proteases in the larval midgut of Helicoverpa armigera in response to a plant protease inhibitor. Insect Biochem Mol Biol. 2015;59:18-29.
Kuwar, S. S., Pauchet, Y., Vogel, H., & Heckel, D. G. (2015). Adaptive regulation of digestive serine proteases in the larval midgut of Helicoverpa armigera in response to a plant protease inhibitor. Insect Biochemistry and Molecular Biology, 59, 18-29. https://doi.org/10.1016/j.ibmb.2015.01.016
Kuwar SS, et al. Adaptive Regulation of Digestive Serine Proteases in the Larval Midgut of Helicoverpa Armigera in Response to a Plant Protease Inhibitor. Insect Biochem Mol Biol. 2015;59:18-29. PubMed PMID: 25662099.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adaptive regulation of digestive serine proteases in the larval midgut of Helicoverpa armigera in response to a plant protease inhibitor. AU - Kuwar,Suyog S, AU - Pauchet,Yannick, AU - Vogel,Heiko, AU - Heckel,David G, Y1 - 2015/02/04/ PY - 2014/09/23/received PY - 2015/01/07/revised PY - 2015/01/09/accepted PY - 2015/2/10/entrez PY - 2015/2/11/pubmed PY - 2016/2/26/medline KW - Helicoverpa armigera KW - Microarray KW - Protease inhibitor KW - Serine protease SP - 18 EP - 29 JF - Insect biochemistry and molecular biology JO - Insect Biochem Mol Biol VL - 59 N2 - Protease inhibitors (PIs) are direct defenses induced by plants in response to herbivory. PIs reduce herbivore digestive efficiency by inhibiting insects' digestive proteases; in turn insects can adapt to PIs by generally increasing protease levels and/or by inducing the expression of PI-insensitive proteases. Helicoverpa armigera, a highly polyphagous lepidopteran insect pest, is known for its ability to adapt to PIs. To advance our molecular and functional understanding of the regulation of digestive proteases, we performed a comprehensive gene expression experiment of H. armigera exposed to soybean Kunitz trypsin inhibitor (SKTI) using a custom-designed microarray. We observed poor larval growth on the SKTI diet until 24 h, however after 48 h larvae attained comparable weight to that of control diet. Although initially the expression of several trypsins and chymotrypsins increased, eventually the expression of some trypsins decreased, while the number of chymotrypsins and their expression increased in response to SKTI. Some of the diverged serine proteases were also differentially expressed. The expression of serine proteases observed using microarrays were further validated by qRT-PCR at different time points (12, 24, 48, 72 and 96 h) after the start of SKTI ingestion. There were also large changes in transcriptional patterns over time in the control diet. Carbohydrate metabolism and immune defense genes were affected in response to SKTI ingestion. Enzyme assays revealed reduced trypsin-specific activity and increased chymotrypsin-specific activity in response to SKTI. The differential regulation of trypsins and chymotrypsins at the transcript and protein levels accompanying a rebound in growth rate indicates that induction of SKTI-insensitive proteases is an effective strategy of H. armigera in coping with this protease inhibitor in its diet. SN - 1879-0240 UR - https://www.unboundmedicine.com/medline/citation/25662099/Adaptive_regulation_of_digestive_serine_proteases_in_the_larval_midgut_of_Helicoverpa_armigera_in_response_to_a_plant_protease_inhibitor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(15)00023-5 DB - PRIME DP - Unbound Medicine ER -