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Testing the SI × SC rule: Pollen-pistil interactions in interspecific crosses between members of the tomato clade (Solanum section Lycopersicon, Solanaceae).
Am J Bot 2015; 102(2):302-11AJ

Abstract

PREMISE OF THE STUDY

Interspecific reproductive barriers (IRBs) act to ensure species integrity by preventing hybridization. Previous studies on interspecific crosses in the tomato clade have focused on the success of fruit and seed set. The SI × SC rule (SI species × SC species crosses are incompatible, but the reciprocal crosses are compatible) often applies to interspecific crosses. Because SI systems in the Solanaceae affect pollen tube growth, we focused on this process in a comprehensive study of interspecific crosses in the tomato clade to test whether the SI × SC rule was always followed.

METHODS

Pollen tube growth was assessed in reciprocal crosses between all 13 species of the tomato clade using fluorescence microscopy.

KEY RESULTS

In crosses between SC and SI species, pollen tube growth follows the SI × SC rule: interspecific pollen tube rejection occurs when SI species are pollinated by SC species, but in the reciprocal crosses (SC × SI), pollen tubes reach ovaries. However, pollen tube rejection occurred in some crosses between pairs of SC species, demonstrating that a fully functional SI system is not necessary for pollen tube rejection in interspecific crosses. Further, gradations in the strength of both pistil and pollen IRBs were revealed in interspecific crosses using SC populations of generally SI species.

CONCLUSION

The SI × SC rule explains many of the compatibility relations in the tomato clade, but exceptions occur with more recently evolved SC species and accessions, revealing differences in strength of both pistil and pollen IRBs.

Authors+Show Affiliations

Department of Biology, Colorado State University, Fort Collins, Colorado 80523-1878 USA.Department of Biology, Colorado State University, Fort Collins, Colorado 80523-1878 USA.Department of Plant Sciences, University of California, Davis, One Shields Avenue, Davis, California 95616 USA.Department of Plant Sciences, University of California, Davis, One Shields Avenue, Davis, California 95616 USA.Department of Biochemistry, University of Missouri-Columbia, 117 Schweitzer Hall, Columbia, Missouri 65211 USA.Department of Biology, Colorado State University, Fort Collins, Colorado 80523-1878 USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25667082

Citation

Baek, You Soon, et al. "Testing the SI × SC Rule: Pollen-pistil Interactions in Interspecific Crosses Between Members of the Tomato Clade (Solanum Section Lycopersicon, Solanaceae)." American Journal of Botany, vol. 102, no. 2, 2015, pp. 302-11.
Baek YS, Covey PA, Petersen JJ, et al. Testing the SI × SC rule: Pollen-pistil interactions in interspecific crosses between members of the tomato clade (Solanum section Lycopersicon, Solanaceae). Am J Bot. 2015;102(2):302-11.
Baek, Y. S., Covey, P. A., Petersen, J. J., Chetelat, R. T., McClure, B., & Bedinger, P. A. (2015). Testing the SI × SC rule: Pollen-pistil interactions in interspecific crosses between members of the tomato clade (Solanum section Lycopersicon, Solanaceae). American Journal of Botany, 102(2), pp. 302-11. doi:10.3732/ajb.1400484.
Baek YS, et al. Testing the SI × SC Rule: Pollen-pistil Interactions in Interspecific Crosses Between Members of the Tomato Clade (Solanum Section Lycopersicon, Solanaceae). Am J Bot. 2015;102(2):302-11. PubMed PMID: 25667082.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Testing the SI × SC rule: Pollen-pistil interactions in interspecific crosses between members of the tomato clade (Solanum section Lycopersicon, Solanaceae). AU - Baek,You Soon, AU - Covey,Paul A, AU - Petersen,Jennifer J, AU - Chetelat,Roger T, AU - McClure,Bruce, AU - Bedinger,Patricia A, Y1 - 2015/01/22/ PY - 2015/2/11/entrez PY - 2015/2/11/pubmed PY - 2015/7/16/medline KW - SI × SC rule KW - Solanaceae KW - Solanum KW - interspecific reproductive barriers KW - pollen–pistil interactions KW - self-incompatibility KW - tomato clade KW - unilateral incompatibility KW - unilateral incongruity KW - wide hybridization KW - wild tomato species SP - 302 EP - 11 JF - American journal of botany JO - Am. J. Bot. VL - 102 IS - 2 N2 - PREMISE OF THE STUDY: Interspecific reproductive barriers (IRBs) act to ensure species integrity by preventing hybridization. Previous studies on interspecific crosses in the tomato clade have focused on the success of fruit and seed set. The SI × SC rule (SI species × SC species crosses are incompatible, but the reciprocal crosses are compatible) often applies to interspecific crosses. Because SI systems in the Solanaceae affect pollen tube growth, we focused on this process in a comprehensive study of interspecific crosses in the tomato clade to test whether the SI × SC rule was always followed. METHODS: Pollen tube growth was assessed in reciprocal crosses between all 13 species of the tomato clade using fluorescence microscopy. KEY RESULTS: In crosses between SC and SI species, pollen tube growth follows the SI × SC rule: interspecific pollen tube rejection occurs when SI species are pollinated by SC species, but in the reciprocal crosses (SC × SI), pollen tubes reach ovaries. However, pollen tube rejection occurred in some crosses between pairs of SC species, demonstrating that a fully functional SI system is not necessary for pollen tube rejection in interspecific crosses. Further, gradations in the strength of both pistil and pollen IRBs were revealed in interspecific crosses using SC populations of generally SI species. CONCLUSION: The SI × SC rule explains many of the compatibility relations in the tomato clade, but exceptions occur with more recently evolved SC species and accessions, revealing differences in strength of both pistil and pollen IRBs. SN - 1537-2197 UR - https://www.unboundmedicine.com/medline/citation/25667082/Testing_the_SI_×_SC_rule:_Pollen_pistil_interactions_in_interspecific_crosses_between_members_of_the_tomato_clade__Solanum_section_Lycopersicon_Solanaceae__ L2 - https://doi.org/10.3732/ajb.1400484 DB - PRIME DP - Unbound Medicine ER -