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Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers.
Poult Sci. 2015 Apr; 94(4):601-11.PS

Abstract

Avian pathogenic Escherichia coli (APEC) causes extensive mortality in poultry flocks, leading to extensive economic losses. To date, little information has been available on the molecular basis of antimicrobial resistance in APEC in Hebei, China. Therefore, the objective of this study was to characterize the virulence and antimicrobial resistance of multidrug-resistant APEC isolated from septicemic broilers at the molecular level. Among 87 nonrepetitive E. coli isolates, 41 (47.1%) carried 3 or more of the APEC virulence genes iroN, ompT, iss, iutA, and hlyF. All 87 APEC isolates showed multidrug-resistant phenotypes, particularly against ampicillin, kanamycin, ciprofloxacin, levofloxacin, streptomycin, gentamycin, ofloxacin, norfloxacin, and ceftriaxone. The β-lactamase-encoding genes blaTEM, blaCMY-2, blaOXA-30, blaCTX-M-15, and blaSHV-2; the aminoglycoside-modifying enzymes (AME) strA, strB, aph(3')-IIa, aac(3)-IIa, aac(6')-Ib, and ant(3″)-Ia; and the plasmid-mediated quinolone resistance (PMQR) genes qnrA, qnrB, and qnrS, were also identified in 66 (75.9%), 65 (74.7%), and 6 (6.9%) isolates, respectively. All isolates were evaluated in terms of replicon type. The plasmid replicons were identified in 63 (72.4%) isolates, and the FIB, B/O, and K replicons were the most present. To the best of our knowledge, this is the first report of molecular characterization of antimicrobial resistance in APEC strains from China.

Authors+Show Affiliations

College of Veterinary Medicine, Hebei Agricultural University; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, 071001 liyurong0312@126.com.College of Veterinary Medicine, Hebei Agricultural University; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, 071001.College of Veterinary Medicine, Hebei Agricultural University; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, 071001.College of Veterinary Medicine, Hebei Agricultural University; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, 071001.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25667425

Citation

Li, Yurong, et al. "Molecular Characterization of Multidrug-resistant Avian Pathogenic Escherichia Coli Isolated From Septicemic Broilers." Poultry Science, vol. 94, no. 4, 2015, pp. 601-11.
Li Y, Chen L, Wu X, et al. Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers. Poult Sci. 2015;94(4):601-11.
Li, Y., Chen, L., Wu, X., & Huo, S. (2015). Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers. Poultry Science, 94(4), 601-11. https://doi.org/10.3382/ps/pev008
Li Y, et al. Molecular Characterization of Multidrug-resistant Avian Pathogenic Escherichia Coli Isolated From Septicemic Broilers. Poult Sci. 2015;94(4):601-11. PubMed PMID: 25667425.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers. AU - Li,Yurong, AU - Chen,Ligong, AU - Wu,Xianjun, AU - Huo,Shuying, Y1 - 2015/02/09/ PY - 2015/2/11/entrez PY - 2015/2/11/pubmed PY - 2015/8/26/medline KW - APEC KW - plasmid KW - resistance gene KW - septicemic broiler KW - virulence SP - 601 EP - 11 JF - Poultry science JO - Poult Sci VL - 94 IS - 4 N2 - Avian pathogenic Escherichia coli (APEC) causes extensive mortality in poultry flocks, leading to extensive economic losses. To date, little information has been available on the molecular basis of antimicrobial resistance in APEC in Hebei, China. Therefore, the objective of this study was to characterize the virulence and antimicrobial resistance of multidrug-resistant APEC isolated from septicemic broilers at the molecular level. Among 87 nonrepetitive E. coli isolates, 41 (47.1%) carried 3 or more of the APEC virulence genes iroN, ompT, iss, iutA, and hlyF. All 87 APEC isolates showed multidrug-resistant phenotypes, particularly against ampicillin, kanamycin, ciprofloxacin, levofloxacin, streptomycin, gentamycin, ofloxacin, norfloxacin, and ceftriaxone. The β-lactamase-encoding genes blaTEM, blaCMY-2, blaOXA-30, blaCTX-M-15, and blaSHV-2; the aminoglycoside-modifying enzymes (AME) strA, strB, aph(3')-IIa, aac(3)-IIa, aac(6')-Ib, and ant(3″)-Ia; and the plasmid-mediated quinolone resistance (PMQR) genes qnrA, qnrB, and qnrS, were also identified in 66 (75.9%), 65 (74.7%), and 6 (6.9%) isolates, respectively. All isolates were evaluated in terms of replicon type. The plasmid replicons were identified in 63 (72.4%) isolates, and the FIB, B/O, and K replicons were the most present. To the best of our knowledge, this is the first report of molecular characterization of antimicrobial resistance in APEC strains from China. SN - 0032-5791 UR - https://www.unboundmedicine.com/medline/citation/25667425/Molecular_characterization_of_multidrug_resistant_avian_pathogenic_Escherichia_coli_isolated_from_septicemic_broilers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0032-5791(19)32442-3 DB - PRIME DP - Unbound Medicine ER -