Tags

Type your tag names separated by a space and hit enter

Role of excitatory amino acid receptors in synaptic transmission in area CA1 of rat hippocampus.
Proc R Soc Lond B Biol Sci. 1989 May 22; 236(1285):373-84.PR

Abstract

The new antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), which blocks responses to kainate and quisqualate, has been used in conjunction with D-2-amino-5-phosphonovalerate (APV), which blocks selectively responses to N-methyl-D-aspartate (NMDA), to determine the role of excitatory amino acid receptors in synaptic transmission. An excitatory postsynaptic potential (EPSP)-inhibitory postsynaptic potential (IPSP) sequence was evoked in CA1 neurons by stimulation of the Schaffer collateral-commissural pathway in rat hippocampal slices. CNQX (10 microM) substantially reduced the EPSP without having any effect on input resistance or membrane potential. The IPSP was also reduced provided that the stimulating electrode was place approximately 1 mm from the recording electrode. The EPSP that remained in the presence of CNQX had characteristics of an NMDA receptor-mediated potential; it had a slow timecourse, summated at high frequencies, was blocked reversibly by APV, increased greatly in size in Mg2+-free medium, and showed an anomalous voltage dependence in Mg2+-containing medium. In the presence of CNQX, an APV-sensitive polysynaptic GABAergic IPSP could be evoked, indicating that NMDA receptors can mediate suprathreshold EPSPS in inhibitory interneurons. It is suggested that either NMDA or non-NMDA receptors can, under different circumstances, mediate the synaptic excitation of pyramidal neurons and inhibitory interneurons in area CA1 of the hippocampus.

Authors+Show Affiliations

Department of Pharmacology, University of Bristol, School of Medical Sciences, U.K.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2567518

Citation

Davies, S N., and G L. Collingridge. "Role of Excitatory Amino Acid Receptors in Synaptic Transmission in Area CA1 of Rat Hippocampus." Proceedings of the Royal Society of London. Series B, Biological Sciences, vol. 236, no. 1285, 1989, pp. 373-84.
Davies SN, Collingridge GL. Role of excitatory amino acid receptors in synaptic transmission in area CA1 of rat hippocampus. Proc R Soc Lond B Biol Sci. 1989;236(1285):373-84.
Davies, S. N., & Collingridge, G. L. (1989). Role of excitatory amino acid receptors in synaptic transmission in area CA1 of rat hippocampus. Proceedings of the Royal Society of London. Series B, Biological Sciences, 236(1285), 373-84.
Davies SN, Collingridge GL. Role of Excitatory Amino Acid Receptors in Synaptic Transmission in Area CA1 of Rat Hippocampus. Proc R Soc Lond B Biol Sci. 1989 May 22;236(1285):373-84. PubMed PMID: 2567518.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of excitatory amino acid receptors in synaptic transmission in area CA1 of rat hippocampus. AU - Davies,S N, AU - Collingridge,G L, PY - 1989/5/22/pubmed PY - 1989/5/22/medline PY - 1989/5/22/entrez SP - 373 EP - 84 JF - Proceedings of the Royal Society of London. Series B, Biological sciences JO - Proc R Soc Lond B Biol Sci VL - 236 IS - 1285 N2 - The new antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), which blocks responses to kainate and quisqualate, has been used in conjunction with D-2-amino-5-phosphonovalerate (APV), which blocks selectively responses to N-methyl-D-aspartate (NMDA), to determine the role of excitatory amino acid receptors in synaptic transmission. An excitatory postsynaptic potential (EPSP)-inhibitory postsynaptic potential (IPSP) sequence was evoked in CA1 neurons by stimulation of the Schaffer collateral-commissural pathway in rat hippocampal slices. CNQX (10 microM) substantially reduced the EPSP without having any effect on input resistance or membrane potential. The IPSP was also reduced provided that the stimulating electrode was place approximately 1 mm from the recording electrode. The EPSP that remained in the presence of CNQX had characteristics of an NMDA receptor-mediated potential; it had a slow timecourse, summated at high frequencies, was blocked reversibly by APV, increased greatly in size in Mg2+-free medium, and showed an anomalous voltage dependence in Mg2+-containing medium. In the presence of CNQX, an APV-sensitive polysynaptic GABAergic IPSP could be evoked, indicating that NMDA receptors can mediate suprathreshold EPSPS in inhibitory interneurons. It is suggested that either NMDA or non-NMDA receptors can, under different circumstances, mediate the synaptic excitation of pyramidal neurons and inhibitory interneurons in area CA1 of the hippocampus. SN - 0950-1193 UR - https://www.unboundmedicine.com/medline/citation/2567518/Role_of_excitatory_amino_acid_receptors_in_synaptic_transmission_in_area_CA1_of_rat_hippocampus_ L2 - https://royalsocietypublishing.org/doi/10.1098/rspb.1989.0028?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -