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Insulin antagonism of dexamethasone induction of tyrosine aminotransferase in cultured fetal hepatocytes. A correlation between enzyme activity, synthesis, level of messenger RNA and transcription.
Eur J Biochem. 1989 Jun 15; 182(2):429-35.EJ

Abstract

Previous studies have shown that insulin depresses the induction of tyrosine aminotransferase by glucocorticoids in cultured fetal rat hepatocytes. However, the site at which this inhibitory effect is exerted was not elucidated, since only enzyme activity was determined in such studies. Therefore, the effect of insulin on tyrosine aminotransferase synthesis, the level of its mRNA as well as the rate of transcription of the gene in isolated nuclei have been determined. The results obtained indicate that in cultures exposed to dexamethasone, Bt2cAMP, insulin and combinations of these additives, there is an excellent correlation between the enzyme activity, enzyme synthesis and the level of mRNA. Run-on transcription experiments indicate that the reduction in the level of mRNA by insulin in dexamethasone-supplemented cultures is the result of a diminished rate of gene transcription.

Authors+Show Affiliations

Cake MH
School of Biological and Environmental Sciences, Murdoch University, Western Australia.
Ho KK
No affiliation info available
Shelly L
No affiliation info available
Milward E
No affiliation info available
Yeoh GC
No affiliation info available

MeSH

AnimalsCells, CulturedDexamethasoneDrug AntagonismEnzyme InductionFetusInsulinLiverRNA, MessengerRatsRats, Inbred StrainsTranscription, GeneticTyrosine Transaminase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2567669

Citation

Cake, M H., et al. "Insulin Antagonism of Dexamethasone Induction of Tyrosine Aminotransferase in Cultured Fetal Hepatocytes. a Correlation Between Enzyme Activity, Synthesis, Level of Messenger RNA and Transcription." European Journal of Biochemistry, vol. 182, no. 2, 1989, pp. 429-35.
Cake MH, Ho KK, Shelly L, et al. Insulin antagonism of dexamethasone induction of tyrosine aminotransferase in cultured fetal hepatocytes. A correlation between enzyme activity, synthesis, level of messenger RNA and transcription. Eur J Biochem. 1989;182(2):429-35.
Cake, M. H., Ho, K. K., Shelly, L., Milward, E., & Yeoh, G. C. (1989). Insulin antagonism of dexamethasone induction of tyrosine aminotransferase in cultured fetal hepatocytes. A correlation between enzyme activity, synthesis, level of messenger RNA and transcription. European Journal of Biochemistry, 182(2), 429-35.
Cake MH, et al. Insulin Antagonism of Dexamethasone Induction of Tyrosine Aminotransferase in Cultured Fetal Hepatocytes. a Correlation Between Enzyme Activity, Synthesis, Level of Messenger RNA and Transcription. Eur J Biochem. 1989 Jun 15;182(2):429-35. PubMed PMID: 2567669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Insulin antagonism of dexamethasone induction of tyrosine aminotransferase in cultured fetal hepatocytes. A correlation between enzyme activity, synthesis, level of messenger RNA and transcription. AU - Cake,M H, AU - Ho,K K, AU - Shelly,L, AU - Milward,E, AU - Yeoh,G C, PY - 1989/6/15/pubmed PY - 1989/6/15/medline PY - 1989/6/15/entrez SP - 429 EP - 35 JF - European journal of biochemistry JO - Eur J Biochem VL - 182 IS - 2 N2 - Previous studies have shown that insulin depresses the induction of tyrosine aminotransferase by glucocorticoids in cultured fetal rat hepatocytes. However, the site at which this inhibitory effect is exerted was not elucidated, since only enzyme activity was determined in such studies. Therefore, the effect of insulin on tyrosine aminotransferase synthesis, the level of its mRNA as well as the rate of transcription of the gene in isolated nuclei have been determined. The results obtained indicate that in cultures exposed to dexamethasone, Bt2cAMP, insulin and combinations of these additives, there is an excellent correlation between the enzyme activity, enzyme synthesis and the level of mRNA. Run-on transcription experiments indicate that the reduction in the level of mRNA by insulin in dexamethasone-supplemented cultures is the result of a diminished rate of gene transcription. SN - 0014-2956 UR - https://www.unboundmedicine.com/medline/citation/2567669/Insulin_antagonism_of_dexamethasone_induction_of_tyrosine_aminotransferase_in_cultured_fetal_hepatocytes__A_correlation_between_enzyme_activity_synthesis_level_of_messenger_RNA_and_transcription_ DB - PRIME DP - Unbound Medicine ER -