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Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice.
Int J Nanomedicine. 2015; 10:297-303.IJ

Abstract

Diclofenac is a nonsteroidal anti-inflammatory drug (NSAID) that exhibits anti-inflammatory, antinociceptive, and antipyretic activities. Liposomes have been shown to improve the therapeutic efficacy of encapsulated drugs. The present study was conducted to compare the antinociceptive properties between liposome-encapsulated and free-form diclofenac in vivo via different nociceptive assay models. Liposome-encapsulated diclofenac was prepared using the commercialized proliposome method. Antinociceptive effects of liposome-encapsulated and free-form diclofenac were evaluated using formalin test, acetic acid-induced abdominal writhing test, Randall-Selitto paw pressure test, and plantar test. The results of the writhing test showed a significant reduction of abdominal constriction in all treatment groups in a dose-dependent manner. The 20 mg/kg liposome-encapsulated diclofenac demonstrated the highest antinociceptive effect at 78.97% compared with 55.89% in the free-form group at equivalent dosage. Both liposome-encapsulated and free-form diclofenac produced significant results in the late phase of formalin assay at a dose of 20 mg/kg, with antinociception percentages of 78.84% and 60.71%, respectively. Significant results of antinociception were also observed in both hyperalgesia assays. For Randall-Sellito assay, the highest antinociception effect of 71.38% was achieved with 20 mg/kg liposome-encapsulated diclofenac, while the lowest antinociceptive effect of 17.32% was recorded with 0 mg/kg liposome formulation, whereas in the plantar test, the highest antinociceptive effect was achieved at 56.7% with 20 mg/kg liposome-encapsulated diclofenac, and the lowest effect was shown with 0 mg/kg liposome formulation of 8.89%. The present study suggests that liposome-encapsulated diclofenac exhibits higher antinociceptive efficacy in a dose-dependent manner in comparison with free-form diclofenac.

Authors+Show Affiliations

Department of Biomedical Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.Department of Biomedical Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.Department of Biomedical Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia ; InQpharm Group, Kuala Lumpur, Malaysia.Department of Human Anatomy, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.Department of Biomedical Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.Department of Biomedical Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia ; Halal Product Research Institute, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25678786

Citation

Goh, Jun Zheng, et al. "Evaluation of Antinociceptive Activity of Nanoliposome-encapsulated and Free-form Diclofenac in Rats and Mice." International Journal of Nanomedicine, vol. 10, 2015, pp. 297-303.
Goh JZ, Tang SN, Chiong HS, et al. Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice. Int J Nanomedicine. 2015;10:297-303.
Goh, J. Z., Tang, S. N., Chiong, H. S., Yong, Y. K., Zuraini, A., & Hakim, M. N. (2015). Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice. International Journal of Nanomedicine, 10, 297-303. https://doi.org/10.2147/IJN.S75545
Goh JZ, et al. Evaluation of Antinociceptive Activity of Nanoliposome-encapsulated and Free-form Diclofenac in Rats and Mice. Int J Nanomedicine. 2015;10:297-303. PubMed PMID: 25678786.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice. AU - Goh,Jun Zheng, AU - Tang,Sook Nai, AU - Chiong,Hoe Siong, AU - Yong,Yoke Keong, AU - Zuraini,Ahmad, AU - Hakim,Muhammad Nazrul, Y1 - 2014/12/31/ PY - 2015/2/14/entrez PY - 2015/2/14/pubmed PY - 2016/8/17/medline KW - antinociceptive KW - diclofenac KW - efficacy KW - liposome KW - nanoencapsulation SP - 297 EP - 303 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 10 N2 - Diclofenac is a nonsteroidal anti-inflammatory drug (NSAID) that exhibits anti-inflammatory, antinociceptive, and antipyretic activities. Liposomes have been shown to improve the therapeutic efficacy of encapsulated drugs. The present study was conducted to compare the antinociceptive properties between liposome-encapsulated and free-form diclofenac in vivo via different nociceptive assay models. Liposome-encapsulated diclofenac was prepared using the commercialized proliposome method. Antinociceptive effects of liposome-encapsulated and free-form diclofenac were evaluated using formalin test, acetic acid-induced abdominal writhing test, Randall-Selitto paw pressure test, and plantar test. The results of the writhing test showed a significant reduction of abdominal constriction in all treatment groups in a dose-dependent manner. The 20 mg/kg liposome-encapsulated diclofenac demonstrated the highest antinociceptive effect at 78.97% compared with 55.89% in the free-form group at equivalent dosage. Both liposome-encapsulated and free-form diclofenac produced significant results in the late phase of formalin assay at a dose of 20 mg/kg, with antinociception percentages of 78.84% and 60.71%, respectively. Significant results of antinociception were also observed in both hyperalgesia assays. For Randall-Sellito assay, the highest antinociception effect of 71.38% was achieved with 20 mg/kg liposome-encapsulated diclofenac, while the lowest antinociceptive effect of 17.32% was recorded with 0 mg/kg liposome formulation, whereas in the plantar test, the highest antinociceptive effect was achieved at 56.7% with 20 mg/kg liposome-encapsulated diclofenac, and the lowest effect was shown with 0 mg/kg liposome formulation of 8.89%. The present study suggests that liposome-encapsulated diclofenac exhibits higher antinociceptive efficacy in a dose-dependent manner in comparison with free-form diclofenac. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/25678786/Evaluation_of_antinociceptive_activity_of_nanoliposome_encapsulated_and_free_form_diclofenac_in_rats_and_mice_ L2 - https://dx.doi.org/10.2147/IJN.S75545 DB - PRIME DP - Unbound Medicine ER -