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Development of an equine groove model to induce metacarpophalangeal osteoarthritis: a pilot study on 6 horses.
PLoS One. 2015; 10(2):e0115089.Plos

Abstract

The aim of this work was to develop an equine metacarpophalangeal joint model that induces osteoarthritis that is not primarily mediated by instability or inflammation. The study involved six Standardbred horses. Standardized cartilage surface damage or "grooves" were created arthroscopically on the distal dorsal aspect of the lateral and medial metacarpal condyles of a randomly chosen limb. The contralateral limb was sham operated. After 2 weeks of stall rest, horses were trotted 30 minutes every other day for 8 weeks, then evaluated for lameness and radiographed. Synovial fluid was analyzed for cytology and biomarkers. At 10 weeks post-surgery, horses were euthanized for macroscopic and histologic joint evaluation. Arthroscopic grooving allowed precise and identical damage to the cartilage of all animals. Under the controlled exercise regime, this osteoarthritis groove model displayed significant radiographic, macroscopic, and microscopic degenerative and reactive changes. Histology demonstrated consistent surgically induced grooves limited to non-calcified cartilage and accompanied by secondary adjacent cartilage lesions, chondrocyte necrosis, chondrocyte clusters, cartilage matrix softening, fissuring, mild subchondral bone inflammation, edema, and osteoblastic margination. Synovial fluid biochemistry and cytology demonstrated significantly elevated total protein without an increase in prostaglandin E2, neutrophils, or chondrocytes. This equine metacarpophalangeal groove model demonstrated that standardized non-calcified cartilage damage accompanied by exercise triggered altered osteochondral morphology and cartilage degeneration with minimal or inefficient repair and little inflammatory response. This model, if validated, would allow for assessment of disease processes and the effects of therapy.

Authors+Show Affiliations

Equine Research Centre, University of Lyon, VetAgro Sup, Veterinary Campus of Lyon, GREMERES-ICE, Marcy l'Etoile, France.Equine Research Centre, University of Lyon, VetAgro Sup, Veterinary Campus of Lyon, GREMERES-ICE, Marcy l'Etoile, France.Equine Research Centre, University of Lyon, VetAgro Sup, Veterinary Campus of Lyon, GREMERES-ICE, Marcy l'Etoile, France.Exploratory Unit, Sanofi-aventis Recherche, Montpellier, France.Exploratory Unit, Sanofi-aventis Recherche, Montpellier, France.Exploratory Unit, Sanofi-aventis Recherche, Montpellier, France.Exploratory Unit, Sanofi-aventis Recherche, Montpellier, France.Equine Research Centre, University of Lyon, VetAgro Sup, Veterinary Campus of Lyon, GREMERES-ICE, Marcy l'Etoile, France.Exploratory Unit, Sanofi-aventis Recherche, Montpellier, France.Clinical unit for osteoarticular diseases, CHU Lapeyronie University Hospital, Montpellier, France.Exploratory Unit, Sanofi-aventis Recherche, Montpellier, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25680102

Citation

Maninchedda, Ugo, et al. "Development of an Equine Groove Model to Induce Metacarpophalangeal Osteoarthritis: a Pilot Study On 6 Horses." PloS One, vol. 10, no. 2, 2015, pp. e0115089.
Maninchedda U, Lepage OM, Gangl M, et al. Development of an equine groove model to induce metacarpophalangeal osteoarthritis: a pilot study on 6 horses. PLoS ONE. 2015;10(2):e0115089.
Maninchedda, U., Lepage, O. M., Gangl, M., Hilairet, S., Remandet, B., Meot, F., Penarier, G., Segard, E., Cortez, P., Jorgensen, C., & Steinberg, R. (2015). Development of an equine groove model to induce metacarpophalangeal osteoarthritis: a pilot study on 6 horses. PloS One, 10(2), e0115089. https://doi.org/10.1371/journal.pone.0115089
Maninchedda U, et al. Development of an Equine Groove Model to Induce Metacarpophalangeal Osteoarthritis: a Pilot Study On 6 Horses. PLoS ONE. 2015;10(2):e0115089. PubMed PMID: 25680102.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of an equine groove model to induce metacarpophalangeal osteoarthritis: a pilot study on 6 horses. AU - Maninchedda,Ugo, AU - Lepage,Olivier M, AU - Gangl,Monika, AU - Hilairet,Sandrine, AU - Remandet,Bernard, AU - Meot,Francoise, AU - Penarier,Geraldine, AU - Segard,Emilie, AU - Cortez,Pierre, AU - Jorgensen,Christian, AU - Steinberg,Régis, Y1 - 2015/02/13/ PY - 2014/09/04/received PY - 2014/11/10/accepted PY - 2015/2/14/entrez PY - 2015/2/14/pubmed PY - 2016/1/14/medline SP - e0115089 EP - e0115089 JF - PloS one JO - PLoS ONE VL - 10 IS - 2 N2 - The aim of this work was to develop an equine metacarpophalangeal joint model that induces osteoarthritis that is not primarily mediated by instability or inflammation. The study involved six Standardbred horses. Standardized cartilage surface damage or "grooves" were created arthroscopically on the distal dorsal aspect of the lateral and medial metacarpal condyles of a randomly chosen limb. The contralateral limb was sham operated. After 2 weeks of stall rest, horses were trotted 30 minutes every other day for 8 weeks, then evaluated for lameness and radiographed. Synovial fluid was analyzed for cytology and biomarkers. At 10 weeks post-surgery, horses were euthanized for macroscopic and histologic joint evaluation. Arthroscopic grooving allowed precise and identical damage to the cartilage of all animals. Under the controlled exercise regime, this osteoarthritis groove model displayed significant radiographic, macroscopic, and microscopic degenerative and reactive changes. Histology demonstrated consistent surgically induced grooves limited to non-calcified cartilage and accompanied by secondary adjacent cartilage lesions, chondrocyte necrosis, chondrocyte clusters, cartilage matrix softening, fissuring, mild subchondral bone inflammation, edema, and osteoblastic margination. Synovial fluid biochemistry and cytology demonstrated significantly elevated total protein without an increase in prostaglandin E2, neutrophils, or chondrocytes. This equine metacarpophalangeal groove model demonstrated that standardized non-calcified cartilage damage accompanied by exercise triggered altered osteochondral morphology and cartilage degeneration with minimal or inefficient repair and little inflammatory response. This model, if validated, would allow for assessment of disease processes and the effects of therapy. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25680102/Development_of_an_equine_groove_model_to_induce_metacarpophalangeal_osteoarthritis:_a_pilot_study_on_6_horses_ L2 - http://dx.plos.org/10.1371/journal.pone.0115089 DB - PRIME DP - Unbound Medicine ER -