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Phenotypes of ATP-activated current associated with their genotypes of P2X1-6 subunits in neurons innervating tooth-pulp.
Biochem Biophys Res Commun 2015; 458(3):596-600BB

Abstract

To explore the association of the phenotype of ATP-activated current with the genotype of P2X1-6 subunits in nociceptors, we developed a method that allows us to label nociceptive neurons innervating tooth-pulp in rat trigeminal ganglion (TG) neurons using a retrograde fluorescence-tracing method, to record ATP-activated current in freshly isolated fluorescence-labeled neurons, and then to conduct single cell immunohistochemical staining for P2X1-6 subunits in the same neuron. We found that fast application of 100 μM ATP to fluorescence-traced TG neurons produced robust inward current in 87% (96/110) of cells tested. The diameter of cells varied from 16 to 56 μm. Three types of ATP-activated current (F, I and S) were recorded with distinct rise times of the current (R10-90, P < 0.05). There was a positive correlation between the cell diameter and the value of R10-90 (P < 0.05): the value of R10-90 increased with increases in the cell diameter. Cells responsive to ATP with the type F current mainly showed positive staining for P2X3 and P2X5, but negative staining for P2X2; cells responsive to ATP with the type I current showed positive staining for P2X1-3 and P2X5, but negative staining for P2X4; and cells responsive to ATP with the type S current showed positive staining for P2X1-5, but negative staining for P2X6. The present findings suggest that in addition to P2X3 subunits, P2X5 subunits are also involved in the generation of the F type of ATP-activated current in small-sized nociceptive neurons. In addition to the P2X2/3 subunit-containing channels, more complex uncharacterized combinations of P2X1-5 subunits exist in native medium-sized nociceptive neurons exhibiting the I and S types of ATP-activated current. In addition, the P2X6 subunit is not a main subunit involved in the nociceptive signal in rat TG neurons innervating tooth-pulp.

Authors+Show Affiliations

Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, Hubei Province, China; School of Medicine, Jianghan University, Wuhan, 430056, China.Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, Hubei Province, China.Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, Hubei Province, China.Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, Hubei Province, China.School of Medicine, Jianghan University, Wuhan, 430056, China.Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, Hubei Province, China.Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, Hubei Province, China.Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, Hubei Province, China. Electronic address: licy.whibs@aliyun.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25680470

Citation

Liu, Yuwei, et al. "Phenotypes of ATP-activated Current Associated With Their Genotypes of P2X1-6 Subunits in Neurons Innervating Tooth-pulp." Biochemical and Biophysical Research Communications, vol. 458, no. 3, 2015, pp. 596-600.
Liu Y, Tian X, Wu Y, et al. Phenotypes of ATP-activated current associated with their genotypes of P2X1-6 subunits in neurons innervating tooth-pulp. Biochem Biophys Res Commun. 2015;458(3):596-600.
Liu, Y., Tian, X., Wu, Y., Chen, L., Yi, C. L., Li, Z. W., ... Li, C. Y. (2015). Phenotypes of ATP-activated current associated with their genotypes of P2X1-6 subunits in neurons innervating tooth-pulp. Biochemical and Biophysical Research Communications, 458(3), pp. 596-600. doi:10.1016/j.bbrc.2015.01.156.
Liu Y, et al. Phenotypes of ATP-activated Current Associated With Their Genotypes of P2X1-6 Subunits in Neurons Innervating Tooth-pulp. Biochem Biophys Res Commun. 2015 Mar 13;458(3):596-600. PubMed PMID: 25680470.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypes of ATP-activated current associated with their genotypes of P2X1-6 subunits in neurons innervating tooth-pulp. AU - Liu,Yuwei, AU - Tian,Xiang, AU - Wu,Yuxiang, AU - Chen,Lin, AU - Yi,Chu-Li, AU - Li,Zhi-Wang, AU - Zhang,Ying, AU - Li,Chao-Ying, Y1 - 2015/02/11/ PY - 2015/01/23/received PY - 2015/01/29/accepted PY - 2015/2/15/entrez PY - 2015/2/15/pubmed PY - 2015/5/13/medline KW - Immunohistochemistry KW - P2X KW - Retrograde fluorescence-tracing KW - Tooth-pulp KW - Trigeminal ganglion SP - 596 EP - 600 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 458 IS - 3 N2 - To explore the association of the phenotype of ATP-activated current with the genotype of P2X1-6 subunits in nociceptors, we developed a method that allows us to label nociceptive neurons innervating tooth-pulp in rat trigeminal ganglion (TG) neurons using a retrograde fluorescence-tracing method, to record ATP-activated current in freshly isolated fluorescence-labeled neurons, and then to conduct single cell immunohistochemical staining for P2X1-6 subunits in the same neuron. We found that fast application of 100 μM ATP to fluorescence-traced TG neurons produced robust inward current in 87% (96/110) of cells tested. The diameter of cells varied from 16 to 56 μm. Three types of ATP-activated current (F, I and S) were recorded with distinct rise times of the current (R10-90, P < 0.05). There was a positive correlation between the cell diameter and the value of R10-90 (P < 0.05): the value of R10-90 increased with increases in the cell diameter. Cells responsive to ATP with the type F current mainly showed positive staining for P2X3 and P2X5, but negative staining for P2X2; cells responsive to ATP with the type I current showed positive staining for P2X1-3 and P2X5, but negative staining for P2X4; and cells responsive to ATP with the type S current showed positive staining for P2X1-5, but negative staining for P2X6. The present findings suggest that in addition to P2X3 subunits, P2X5 subunits are also involved in the generation of the F type of ATP-activated current in small-sized nociceptive neurons. In addition to the P2X2/3 subunit-containing channels, more complex uncharacterized combinations of P2X1-5 subunits exist in native medium-sized nociceptive neurons exhibiting the I and S types of ATP-activated current. In addition, the P2X6 subunit is not a main subunit involved in the nociceptive signal in rat TG neurons innervating tooth-pulp. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/25680470/Phenotypes_of_ATP_activated_current_associated_with_their_genotypes_of_P2X1_6_subunits_in_neurons_innervating_tooth_pulp_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(15)00222-3 DB - PRIME DP - Unbound Medicine ER -