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Evaluation of DNA damage and antioxidant system induced by di-n-butyl phthalates exposure in earthworms (Eisenia fetida).
Ecotoxicol Environ Saf. 2015 May; 115:75-82.EE

Abstract

Di-n-butyl phthalates (DBP) are recognized as ubiquitous contaminants in soil and adversely impact the health of organisms. The effect of DBP on the activity of antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT), malondialdehyde (MDA) content and DNA damage were used as biomarkers to analyze the relationship between DNA damage and oxidative stress and to evaluate the genotoxic effect of DBP on earthworms (Eisenia fetida). DBP was added to artificial soil in the amounts of 0, 5, 10, 50 and 100mg per kg of soil. Earthworm tissues exposed to each treatment were collected on the 7th, 14th, 21st, and 28th day of the treatment. The results showed that SOD and CAT levels were significantly inhibited in the 100mgkg(-1) treatment group on day 28. MDA content in treatment groups was higher than in the control group throughout the exposure time, suggesting that DBP may lead to oxidative stress in cells. A dose-response relationship existed between DNA damage and total soil DBP levels. The comet assay showed that increasing concentrations of DBP resulted in a gradual increase in the OTM, Comet Tail Length and Tail DNA %. The degree of DNA damage was increased with increasing concentration of DBP. These results suggested that DBP induced serious oxidative damage on earthworms and induced the formation of reactive oxygen species (ROS) in earthworms. The excessive generation of ROS caused damage to vital macromolecules including lipids and DNA. DBP in the soils were responsible for the exerting genotoxic effects on earthworms.

Authors+Show Affiliations

College of Resources and Environment, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer, Key Laboratory of Colleges and Universities in Shandong Province Agricultural Environment, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China.College of Resources and Environment, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer, Key Laboratory of Colleges and Universities in Shandong Province Agricultural Environment, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China. Electronic address: lgd107@sdau.edu.cn.College of Resources and Environment, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer, Key Laboratory of Colleges and Universities in Shandong Province Agricultural Environment, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China.College of Resources and Environment, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer, Key Laboratory of Colleges and Universities in Shandong Province Agricultural Environment, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China.College of Resources and Environment, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer, Key Laboratory of Colleges and Universities in Shandong Province Agricultural Environment, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China.College of Resources and Environment, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer, Key Laboratory of Colleges and Universities in Shandong Province Agricultural Environment, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China.College of Resources and Environment, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer, Key Laboratory of Colleges and Universities in Shandong Province Agricultural Environment, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25682584

Citation

Du, Li, et al. "Evaluation of DNA Damage and Antioxidant System Induced By Di-n-butyl Phthalates Exposure in Earthworms (Eisenia Fetida)." Ecotoxicology and Environmental Safety, vol. 115, 2015, pp. 75-82.
Du L, Li G, Liu M, et al. Evaluation of DNA damage and antioxidant system induced by di-n-butyl phthalates exposure in earthworms (Eisenia fetida). Ecotoxicol Environ Saf. 2015;115:75-82.
Du, L., Li, G., Liu, M., Li, Y., Yin, S., Zhao, J., & Zhang, X. (2015). Evaluation of DNA damage and antioxidant system induced by di-n-butyl phthalates exposure in earthworms (Eisenia fetida). Ecotoxicology and Environmental Safety, 115, 75-82. https://doi.org/10.1016/j.ecoenv.2015.01.031
Du L, et al. Evaluation of DNA Damage and Antioxidant System Induced By Di-n-butyl Phthalates Exposure in Earthworms (Eisenia Fetida). Ecotoxicol Environ Saf. 2015;115:75-82. PubMed PMID: 25682584.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of DNA damage and antioxidant system induced by di-n-butyl phthalates exposure in earthworms (Eisenia fetida). AU - Du,Li, AU - Li,Guangde, AU - Liu,Mingming, AU - Li,Yanqiang, AU - Yin,Suzhen, AU - Zhao,Jie, AU - Zhang,Xinyi, Y1 - 2015/02/13/ PY - 2014/11/14/received PY - 2015/01/28/revised PY - 2015/01/31/accepted PY - 2015/2/16/entrez PY - 2015/2/16/pubmed PY - 2015/10/24/medline KW - DNA damage KW - Di-n-butyl phthalate KW - Earthworm (Eisenia fetida) KW - Genotoxicity KW - Oxidative stress SP - 75 EP - 82 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 115 N2 - Di-n-butyl phthalates (DBP) are recognized as ubiquitous contaminants in soil and adversely impact the health of organisms. The effect of DBP on the activity of antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT), malondialdehyde (MDA) content and DNA damage were used as biomarkers to analyze the relationship between DNA damage and oxidative stress and to evaluate the genotoxic effect of DBP on earthworms (Eisenia fetida). DBP was added to artificial soil in the amounts of 0, 5, 10, 50 and 100mg per kg of soil. Earthworm tissues exposed to each treatment were collected on the 7th, 14th, 21st, and 28th day of the treatment. The results showed that SOD and CAT levels were significantly inhibited in the 100mgkg(-1) treatment group on day 28. MDA content in treatment groups was higher than in the control group throughout the exposure time, suggesting that DBP may lead to oxidative stress in cells. A dose-response relationship existed between DNA damage and total soil DBP levels. The comet assay showed that increasing concentrations of DBP resulted in a gradual increase in the OTM, Comet Tail Length and Tail DNA %. The degree of DNA damage was increased with increasing concentration of DBP. These results suggested that DBP induced serious oxidative damage on earthworms and induced the formation of reactive oxygen species (ROS) in earthworms. The excessive generation of ROS caused damage to vital macromolecules including lipids and DNA. DBP in the soils were responsible for the exerting genotoxic effects on earthworms. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/25682584/Evaluation_of_DNA_damage_and_antioxidant_system_induced_by_di_n_butyl_phthalates_exposure_in_earthworms__Eisenia_fetida__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(15)00041-X DB - PRIME DP - Unbound Medicine ER -