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Genome size shifts: karyotype evolution in Crepis section Neglectoides (Asteraceae).
Plant Biol (Stuttg) 2015; 17(4):775-86PB

Abstract

Plant genome size evolution is a very dynamic process: the ancestral genome of angiosperms was initially most likely small, which led to a tendency towards genome increase during evolution. However, findings in several angiosperm lineages demonstrate mechanisms that also led to genome size contraction. Recent molecular investigations on the Asteraceae genus Crepis suggest that several genomic reduction events have occurred during the evolution of the genus. This study focuses on the Mediterranean Crepis sect. Neglectoides, which includes three species with some of the smallest genomes within the whole genus. Crepis neglecta has the largest genome in sect. Neglectoides, approximately twice the size of the two species Crepis cretica and Crepis hellenica. Whereas C. cretica and C. hellencia are more closely related to each other than to C. neglecta the karyotypes of the latter species and C. cretica are similar, while that of C. hellenica differs considerably. Here, the karyotypic organisation of the three species is investigated with fluorescence in-situ hybridisation and studied in a molecular phylogenetic framework based on the nuclear markers Actin, CHR12, CPN60B, GPCR1 and XTH23. Our findings further corroborate the occurrence of genome size contraction in Crepis, and suggest that the difference in genome size between C. neglecta and C. cretica is mostly due to elimination of dispersed repetitive elements, whereas chromosomal reorganisation was involved in the karyotype formation of C. hellenica.

Authors+Show Affiliations

Botanic Garden and Botanical Museum Berlin-Dahlem, Freie Universität Berlin, Berlin, Germany. Dahlem Centre of Plant Sciences, Freie Universität Berlin, Berlin, Germany.Dahlem Centre of Plant Sciences, Freie Universität Berlin, Berlin, Germany. Institut für Biologie - Angewandte Genetik, Freie Universität Berlin, Berlin, Germany.CNRS, Laboratoire Ecologie, Systématique et Evolution, UMR 8079, Université Paris Sud, Orsay, France. Faculty of Forestry, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.Institute for Biochemistry I, Medical Faculty, University of Cologne, Cologne, Germany. Molecular Biology Group, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany.Botanic Garden and Botanical Museum Berlin-Dahlem, Freie Universität Berlin, Berlin, Germany. AG Spezielle Botanik, Justus-Liebig-Universität Giessen, Giessen, Germany.Dahlem Centre of Plant Sciences, Freie Universität Berlin, Berlin, Germany. Institut für Biologie - Angewandte Genetik, Freie Universität Berlin, Berlin, Germany.Department of Biology, Botanical Institute, University of Patras, Patras, Greece.CNRS, Laboratoire Ecologie, Systématique et Evolution, UMR 8079, Université Paris Sud, Orsay, France.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25683604

Citation

Enke, N, et al. "Genome Size Shifts: Karyotype Evolution in Crepis Section Neglectoides (Asteraceae)." Plant Biology (Stuttgart, Germany), vol. 17, no. 4, 2015, pp. 775-86.
Enke N, Kunze R, Pustahija F, et al. Genome size shifts: karyotype evolution in Crepis section Neglectoides (Asteraceae). Plant Biol (Stuttg). 2015;17(4):775-86.
Enke, N., Kunze, R., Pustahija, F., Glöckner, G., Zimmermann, J., Oberländer, J., ... Siljak-Yakovlev, S. (2015). Genome size shifts: karyotype evolution in Crepis section Neglectoides (Asteraceae). Plant Biology (Stuttgart, Germany), 17(4), pp. 775-86. doi:10.1111/plb.12318.
Enke N, et al. Genome Size Shifts: Karyotype Evolution in Crepis Section Neglectoides (Asteraceae). Plant Biol (Stuttg). 2015;17(4):775-86. PubMed PMID: 25683604.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome size shifts: karyotype evolution in Crepis section Neglectoides (Asteraceae). AU - Enke,N, AU - Kunze,R, AU - Pustahija,F, AU - Glöckner,G, AU - Zimmermann,J, AU - Oberländer,J, AU - Kamari,G, AU - Siljak-Yakovlev,S, Y1 - 2015/04/09/ PY - 2014/08/12/received PY - 2015/01/19/accepted PY - 2015/2/17/entrez PY - 2015/2/17/pubmed PY - 2016/1/6/medline KW - Crepis KW - fluorescence in situ hybridisation KW - fluorochrome banding KW - genome size KW - heterochromatin and rDNA physical mapping KW - molecular phylogeny KW - nuclear single or low copy genes SP - 775 EP - 86 JF - Plant biology (Stuttgart, Germany) JO - Plant Biol (Stuttg) VL - 17 IS - 4 N2 - Plant genome size evolution is a very dynamic process: the ancestral genome of angiosperms was initially most likely small, which led to a tendency towards genome increase during evolution. However, findings in several angiosperm lineages demonstrate mechanisms that also led to genome size contraction. Recent molecular investigations on the Asteraceae genus Crepis suggest that several genomic reduction events have occurred during the evolution of the genus. This study focuses on the Mediterranean Crepis sect. Neglectoides, which includes three species with some of the smallest genomes within the whole genus. Crepis neglecta has the largest genome in sect. Neglectoides, approximately twice the size of the two species Crepis cretica and Crepis hellenica. Whereas C. cretica and C. hellencia are more closely related to each other than to C. neglecta the karyotypes of the latter species and C. cretica are similar, while that of C. hellenica differs considerably. Here, the karyotypic organisation of the three species is investigated with fluorescence in-situ hybridisation and studied in a molecular phylogenetic framework based on the nuclear markers Actin, CHR12, CPN60B, GPCR1 and XTH23. Our findings further corroborate the occurrence of genome size contraction in Crepis, and suggest that the difference in genome size between C. neglecta and C. cretica is mostly due to elimination of dispersed repetitive elements, whereas chromosomal reorganisation was involved in the karyotype formation of C. hellenica. SN - 1438-8677 UR - https://www.unboundmedicine.com/medline/citation/25683604/Genome_size_shifts:_karyotype_evolution_in_Crepis_section_Neglectoides__Asteraceae__ L2 - https://doi.org/10.1111/plb.12318 DB - PRIME DP - Unbound Medicine ER -