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Enhancement of phosphatidylinositol turnover and cyclic nucleotide accumulation by chronic anethole trithione treatment in rat submaxillary glands.
J Pharm Pharmacol. 1989 Apr; 41(4):247-52.JP

Abstract

The effect of chronic treatment with anethole trithione (ANTT) on the phosphatidylinositol (PI) turnover and cyclic (c)AMP and cGMP accumulation in rat submaxillary glands (SMG) has been compared with the effect of chronic treatment with atropine and a cholinesterase inhibitor, diisopropylfluorophosphate (dyflos, DFP). Experiments were performed 24, 48 and 24 h after the last dose of ANTT, atropine and dyflos, respectively. ANTT and atropine enhanced carbachol-stimulated [32P] incorporation into phosphatidic acid in the SMG slices, while dyflos showed no effect. Pilocarpine-stimulated in-vivo incorporation of [3H]myoinositol into inositol phosphates was significantly enhanced by ANTT, but not by atropine or by dyflos. Phospholipase C-dependent hydrolysis of phosphatidylinositol 4,5-bisphosphate was significantly enhanced by ANTT and atropine, but not by dyflos. Pilocarpine-stimulated in-vivo accumulation of cAMP and cGMP was enhanced by ANTT and atropine, but dyflos reduced cAMP accumulation without affecting cGMP accumulation. The enhancement of PI turnover and cyclic nucleotide accumulation seems to contribute to the development of supersensitivity of the salivary gland caused by chronic treatment with ANTT and atropine, while reduction of cAMP accumulation may be responsible for the subsensitivity caused by dyflos.

Authors+Show Affiliations

Ukai Y
Research Laboratories, Nippon Shinyaku Co. Ltd., Kyoto, Japan.
Taniguchi N
No affiliation info available
Yamazaki A
No affiliation info available
Kimura K
No affiliation info available

MeSH

Anethole TrithioneAnimalsAnisolesAtropineCarbacholCyclic AMPCyclic GMPIn Vitro TechniquesInositolIsoflurophateMalePhosphatidylinositolsPilocarpineRatsRats, Inbred StrainsSubmandibular GlandType C Phospholipases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2568464

Citation

Ukai, Y, et al. "Enhancement of Phosphatidylinositol Turnover and Cyclic Nucleotide Accumulation By Chronic Anethole Trithione Treatment in Rat Submaxillary Glands." The Journal of Pharmacy and Pharmacology, vol. 41, no. 4, 1989, pp. 247-52.
Ukai Y, Taniguchi N, Yamazaki A, et al. Enhancement of phosphatidylinositol turnover and cyclic nucleotide accumulation by chronic anethole trithione treatment in rat submaxillary glands. J Pharm Pharmacol. 1989;41(4):247-52.
Ukai, Y., Taniguchi, N., Yamazaki, A., & Kimura, K. (1989). Enhancement of phosphatidylinositol turnover and cyclic nucleotide accumulation by chronic anethole trithione treatment in rat submaxillary glands. The Journal of Pharmacy and Pharmacology, 41(4), 247-52.
Ukai Y, et al. Enhancement of Phosphatidylinositol Turnover and Cyclic Nucleotide Accumulation By Chronic Anethole Trithione Treatment in Rat Submaxillary Glands. J Pharm Pharmacol. 1989;41(4):247-52. PubMed PMID: 2568464.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhancement of phosphatidylinositol turnover and cyclic nucleotide accumulation by chronic anethole trithione treatment in rat submaxillary glands. AU - Ukai,Y, AU - Taniguchi,N, AU - Yamazaki,A, AU - Kimura,K, PY - 1989/4/1/pubmed PY - 1989/4/1/medline PY - 1989/4/1/entrez SP - 247 EP - 52 JF - The Journal of pharmacy and pharmacology JO - J Pharm Pharmacol VL - 41 IS - 4 N2 - The effect of chronic treatment with anethole trithione (ANTT) on the phosphatidylinositol (PI) turnover and cyclic (c)AMP and cGMP accumulation in rat submaxillary glands (SMG) has been compared with the effect of chronic treatment with atropine and a cholinesterase inhibitor, diisopropylfluorophosphate (dyflos, DFP). Experiments were performed 24, 48 and 24 h after the last dose of ANTT, atropine and dyflos, respectively. ANTT and atropine enhanced carbachol-stimulated [32P] incorporation into phosphatidic acid in the SMG slices, while dyflos showed no effect. Pilocarpine-stimulated in-vivo incorporation of [3H]myoinositol into inositol phosphates was significantly enhanced by ANTT, but not by atropine or by dyflos. Phospholipase C-dependent hydrolysis of phosphatidylinositol 4,5-bisphosphate was significantly enhanced by ANTT and atropine, but not by dyflos. Pilocarpine-stimulated in-vivo accumulation of cAMP and cGMP was enhanced by ANTT and atropine, but dyflos reduced cAMP accumulation without affecting cGMP accumulation. The enhancement of PI turnover and cyclic nucleotide accumulation seems to contribute to the development of supersensitivity of the salivary gland caused by chronic treatment with ANTT and atropine, while reduction of cAMP accumulation may be responsible for the subsensitivity caused by dyflos. SN - 0022-3573 UR - https://www.unboundmedicine.com/medline/citation/2568464/Enhancement_of_phosphatidylinositol_turnover_and_cyclic_nucleotide_accumulation_by_chronic_anethole_trithione_treatment_in_rat_submaxillary_glands_ DB - PRIME DP - Unbound Medicine ER -