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[Up-regulation of intrarenal renin-angiotensin system contributes to renal damage in high-salt induced hypertension rats].
Beijing Da Xue Xue Bao Yi Xue Ban. 2015 Feb 18; 47(1):149-54.BD

Abstract

OBJECTIVE

To test the hypothesis that in a high-salt induced hypertension in normal rats, whether the changes of intrarenal renin-agiotensin system (RAS) play a critical role in renal damage and could be reflected by urinary angiotensinogen (AGT).

METHODS

In the study, 27 normotensive male Wistar-Kyoto rats were divided into control group [0.3% (mass faction) NaCl in chow, n=9, NS], high-salt diet group [8% (mass faction) NaCl in chow, n=9, HS] and high-salt diet with Losartan group [8% (mass faction) NaCl in chow and 20 mg/(kg×d) Losartan in gavages, n=9, HS+L)], and were fed for six weeks. The blood pressure was monitored and urine samples were collected every 2 weeks. AGTs in plasma, kidney and urine were measured by ELISA kits. The renal cortex expression of mRNA and protein of AGT were measured by Real-time PCR and immunohistochemistry (IHC). The renin activity and ANG II were measured by radioimmunoassay (RIA) kits.

RESULTS

Compared with NS, the systolic blood pressure (SBP) [(156 ± 2) mmHg vs. (133 ± 3) mmHg, P<0.05] increased significantly at the end of the 2nd week, and the urinary protein [(14.07 ± 2.84) mg/24 h vs. (7.62 ± 3.02) mg/24 h, P<0.05] increased significantly at the end of the 6th week in HS. Compared with HS, there was no significant difference in SBP (P>0.05) but the proteinuria [(9.69 ± 2.73) mg/24 h vs. (14.07 ± 2.84) mg/24 h, P<0.01] decreased significantly in HS+L. Compared with NS, there was no significant difference in the plasma renin activity, angiotensinogen and ANG II level in HS (P>0.05), but the renal cortex renin content [(8.72 ± 1.98) ng/(mL × h) vs. (4.37 ± 1.26) ng/(mL × h), P<0.05], AGT formation [(4.02 ± 0.60) ng/mg vs. (2.59 ± 0.42) ng/mg, P<0.01], ANG II level [(313.8 ± 48.76) pmol/L vs. (188.9 ± 46.95) pmol/L, P<0.05] were increased significantly in HS, and the urinary AGT and ANG II excretion rates increased significantly (P<0.05). Compared with HS, the plasma renin activity, angiotensinogen and ANG II level were significantly increased (P<0.05), but the renal cortex renin content, AGT formation, ANG II level significantly decreased (P<0.05), and the urinary AGT and ANG II excretion rates decreased significantly in HS+L (P<0.05). The urinary AGT excretion rates were positively correlated with the AGT level in the renal cortex (P<0.05).

CONCLUSION

Up-regulation of intarenal RAS may contribute to renal damage in high-salt induced hypertension rats. Urinary AGT may reflect the status of intrarenal RAS.

Authors+Show Affiliations

Department of Nephrology, Peking University Third Hospital, Beijing 100191, China.Department of Nephrology, Peking University Third Hospital, Beijing 100191, China.Department of Nephrology, Peking University Third Hospital, Beijing 100191, China.Department of Nephrology, Peking University Third Hospital, Beijing 100191, China.Department of Nephrology, Peking University Third Hospital, Beijing 100191, China.Department of Nephrology, Peking University Third Hospital, Beijing 100191, China.Department of Nephrology, Peking University Third Hospital, Beijing 100191, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

25686347

Citation

Wu, Hai-yan, et al. "[Up-regulation of Intrarenal Renin-angiotensin System Contributes to Renal Damage in High-salt Induced Hypertension Rats]." Beijing Da Xue Xue Bao. Yi Xue Ban = Journal of Peking University. Health Sciences, vol. 47, no. 1, 2015, pp. 149-54.
Wu HY, Liang YX, Bai Q, et al. [Up-regulation of intrarenal renin-angiotensin system contributes to renal damage in high-salt induced hypertension rats]. Beijing Da Xue Xue Bao. 2015;47(1):149-54.
Wu, H. Y., Liang, Y. X., Bai, Q., Zhuang, Z., A, L. T., Zheng, D. X., & Wang, Y. (2015). [Up-regulation of intrarenal renin-angiotensin system contributes to renal damage in high-salt induced hypertension rats]. Beijing Da Xue Xue Bao. Yi Xue Ban = Journal of Peking University. Health Sciences, 47(1), 149-54.
Wu HY, et al. [Up-regulation of Intrarenal Renin-angiotensin System Contributes to Renal Damage in High-salt Induced Hypertension Rats]. Beijing Da Xue Xue Bao. 2015 Feb 18;47(1):149-54. PubMed PMID: 25686347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Up-regulation of intrarenal renin-angiotensin system contributes to renal damage in high-salt induced hypertension rats]. AU - Wu,Hai-yan, AU - Liang,Yao-xian, AU - Bai,Qiong, AU - Zhuang,Zhen, AU - A,La-ta, AU - Zheng,Dan-xia, AU - Wang,Yue, PY - 2015/2/17/entrez PY - 2015/2/17/pubmed PY - 2015/10/8/medline SP - 149 EP - 54 JF - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences JO - Beijing Da Xue Xue Bao VL - 47 IS - 1 N2 - OBJECTIVE: To test the hypothesis that in a high-salt induced hypertension in normal rats, whether the changes of intrarenal renin-agiotensin system (RAS) play a critical role in renal damage and could be reflected by urinary angiotensinogen (AGT). METHODS: In the study, 27 normotensive male Wistar-Kyoto rats were divided into control group [0.3% (mass faction) NaCl in chow, n=9, NS], high-salt diet group [8% (mass faction) NaCl in chow, n=9, HS] and high-salt diet with Losartan group [8% (mass faction) NaCl in chow and 20 mg/(kg×d) Losartan in gavages, n=9, HS+L)], and were fed for six weeks. The blood pressure was monitored and urine samples were collected every 2 weeks. AGTs in plasma, kidney and urine were measured by ELISA kits. The renal cortex expression of mRNA and protein of AGT were measured by Real-time PCR and immunohistochemistry (IHC). The renin activity and ANG II were measured by radioimmunoassay (RIA) kits. RESULTS: Compared with NS, the systolic blood pressure (SBP) [(156 ± 2) mmHg vs. (133 ± 3) mmHg, P<0.05] increased significantly at the end of the 2nd week, and the urinary protein [(14.07 ± 2.84) mg/24 h vs. (7.62 ± 3.02) mg/24 h, P<0.05] increased significantly at the end of the 6th week in HS. Compared with HS, there was no significant difference in SBP (P>0.05) but the proteinuria [(9.69 ± 2.73) mg/24 h vs. (14.07 ± 2.84) mg/24 h, P<0.01] decreased significantly in HS+L. Compared with NS, there was no significant difference in the plasma renin activity, angiotensinogen and ANG II level in HS (P>0.05), but the renal cortex renin content [(8.72 ± 1.98) ng/(mL × h) vs. (4.37 ± 1.26) ng/(mL × h), P<0.05], AGT formation [(4.02 ± 0.60) ng/mg vs. (2.59 ± 0.42) ng/mg, P<0.01], ANG II level [(313.8 ± 48.76) pmol/L vs. (188.9 ± 46.95) pmol/L, P<0.05] were increased significantly in HS, and the urinary AGT and ANG II excretion rates increased significantly (P<0.05). Compared with HS, the plasma renin activity, angiotensinogen and ANG II level were significantly increased (P<0.05), but the renal cortex renin content, AGT formation, ANG II level significantly decreased (P<0.05), and the urinary AGT and ANG II excretion rates decreased significantly in HS+L (P<0.05). The urinary AGT excretion rates were positively correlated with the AGT level in the renal cortex (P<0.05). CONCLUSION: Up-regulation of intarenal RAS may contribute to renal damage in high-salt induced hypertension rats. Urinary AGT may reflect the status of intrarenal RAS. SN - 1671-167X UR - https://www.unboundmedicine.com/medline/citation/25686347/[Up_regulation_of_intrarenal_renin_angiotensin_system_contributes_to_renal_damage_in_high_salt_induced_hypertension_rats]_ L2 - http://xuebao.bjmu.edu.cn/EN/Y2015/V47/I1/149 DB - PRIME DP - Unbound Medicine ER -