Tags

Type your tag names separated by a space and hit enter

Study of the concordance between p16 immunohistochemistry and HPV-PCR genotyping for the viral diagnosis of oropharyngeal squamous cell carcinoma.

Abstract

OBJECTIVE

The diagnosis of HPV-related oropharyngeal cancer in clinical practice is based on p16 immunohistochemistry and PCR detection of viral DNA (HPV-PCR). The primary objective of this study was to evaluate the concordance between these 2 diagnostic tests. The secondary objective was to study the clinical characteristics of these patients.

MATERIALS AND METHODS

This single-centre prospective study was conducted between February 2010 and July 2012. Immunohistochemical analysis of p16 and HPV-PCR were performed on tumour biopsies. Concordance was evaluated according to Cohen's kappa coefficient and was interpreted according to the Landis and Koch scale. The patients' clinical data were analysed as a function of the diagnostic test results.

RESULTS

Seventy-one patients were included in this study. The prevalence of HPV was 43.7% according to p16 and 31% according to HPV-PCR. The concordance study revealed a kappa coefficient of 0.615. A tumour of the tonsil or base of the tongue was detected in 100% of p16+/HPV-PCR+ cases. Smoking and alcohol abuse were significantly less frequent among HPV+ patients regardless of the method of detection. These patients were older and presented tumours with a lower grade of histological differentiation.

CONCLUSION

p16 immunohistochemistry or HPV-PCR used alone appear to be insufficient. These results confirm the high prevalence of HPV-related oropharyngeal squamous cell carcinoma (OSCC) and the previously reported specific clinical and histological features, apart from age. It appears essential for future clinical trials to be stratified according to smoking and tumour HPV status, defined by means of reliable virological tests targeting E6/E7 mRNA and no longer a simple positive response to the p16 marker, as is frequently the case at the present time. New tests suitable for use in routine practice therefore need to be developed.

Authors+Show Affiliations

Service d'oto-rhino-laryngologie, CHU de Bordeaux, Hôpital Pellegrin, place Amélie-Raba-Léon, 33056 Bordeaux cedex, France.Service d'oto-rhino-laryngologie, CHU de Bordeaux, Hôpital Pellegrin, place Amélie-Raba-Léon, 33056 Bordeaux cedex, France.Laboratoire de virologie, CHU de Bordeaux, Hôpital Pellegrin, place Amélie-Raba-Léon, 33056 Bordeaux cedex, France.Laboratoire d'anatomie-pathologique, CHU de Bordeaux, Hôpital Pellegrin, place Amélie-Raba-Léon, 33056 Bordeaux cedex, France.Service de chirurgie maxillo-faciale, CHU de Bordeaux, Hôpital Pellegrin, place Amélie-Raba-Léon, 33056 Bordeaux cedex, France.Service d'oto-rhino-laryngologie, CHU de Bordeaux, Hôpital Pellegrin, place Amélie-Raba-Léon, 33056 Bordeaux cedex, France.Service d'oto-rhino-laryngologie, CHU de Bordeaux, Hôpital Pellegrin, place Amélie-Raba-Léon, 33056 Bordeaux cedex, France. Electronic address: erwan.de-mones-del-pujol@chu-bordeaux.fr.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

25687724

Citation

Fonmarty, D, et al. "Study of the Concordance Between P16 Immunohistochemistry and HPV-PCR Genotyping for the Viral Diagnosis of Oropharyngeal Squamous Cell Carcinoma." European Annals of Otorhinolaryngology, Head and Neck Diseases, vol. 132, no. 3, 2015, pp. 135-9.
Fonmarty D, Cherrière S, Fleury H, et al. Study of the concordance between p16 immunohistochemistry and HPV-PCR genotyping for the viral diagnosis of oropharyngeal squamous cell carcinoma. Eur Ann Otorhinolaryngol Head Neck Dis. 2015;132(3):135-9.
Fonmarty, D., Cherrière, S., Fleury, H., Eimer, S., Majoufre-Lefebvre, C., Castetbon, V., & de Monès, E. (2015). Study of the concordance between p16 immunohistochemistry and HPV-PCR genotyping for the viral diagnosis of oropharyngeal squamous cell carcinoma. European Annals of Otorhinolaryngology, Head and Neck Diseases, 132(3), pp. 135-9. doi:10.1016/j.anorl.2015.01.003.
Fonmarty D, et al. Study of the Concordance Between P16 Immunohistochemistry and HPV-PCR Genotyping for the Viral Diagnosis of Oropharyngeal Squamous Cell Carcinoma. Eur Ann Otorhinolaryngol Head Neck Dis. 2015;132(3):135-9. PubMed PMID: 25687724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Study of the concordance between p16 immunohistochemistry and HPV-PCR genotyping for the viral diagnosis of oropharyngeal squamous cell carcinoma. AU - Fonmarty,D, AU - Cherrière,S, AU - Fleury,H, AU - Eimer,S, AU - Majoufre-Lefebvre,C, AU - Castetbon,V, AU - de Monès,E, Y1 - 2015/02/14/ PY - 2015/2/18/entrez PY - 2015/2/18/pubmed PY - 2016/3/26/medline KW - HPV KW - Oropharyngeal cancer KW - P16 KW - PCR SP - 135 EP - 9 JF - European annals of otorhinolaryngology, head and neck diseases JO - Eur Ann Otorhinolaryngol Head Neck Dis VL - 132 IS - 3 N2 - OBJECTIVE: The diagnosis of HPV-related oropharyngeal cancer in clinical practice is based on p16 immunohistochemistry and PCR detection of viral DNA (HPV-PCR). The primary objective of this study was to evaluate the concordance between these 2 diagnostic tests. The secondary objective was to study the clinical characteristics of these patients. MATERIALS AND METHODS: This single-centre prospective study was conducted between February 2010 and July 2012. Immunohistochemical analysis of p16 and HPV-PCR were performed on tumour biopsies. Concordance was evaluated according to Cohen's kappa coefficient and was interpreted according to the Landis and Koch scale. The patients' clinical data were analysed as a function of the diagnostic test results. RESULTS: Seventy-one patients were included in this study. The prevalence of HPV was 43.7% according to p16 and 31% according to HPV-PCR. The concordance study revealed a kappa coefficient of 0.615. A tumour of the tonsil or base of the tongue was detected in 100% of p16+/HPV-PCR+ cases. Smoking and alcohol abuse were significantly less frequent among HPV+ patients regardless of the method of detection. These patients were older and presented tumours with a lower grade of histological differentiation. CONCLUSION: p16 immunohistochemistry or HPV-PCR used alone appear to be insufficient. These results confirm the high prevalence of HPV-related oropharyngeal squamous cell carcinoma (OSCC) and the previously reported specific clinical and histological features, apart from age. It appears essential for future clinical trials to be stratified according to smoking and tumour HPV status, defined by means of reliable virological tests targeting E6/E7 mRNA and no longer a simple positive response to the p16 marker, as is frequently the case at the present time. New tests suitable for use in routine practice therefore need to be developed. SN - 1879-730X UR - https://www.unboundmedicine.com/medline/citation/25687724/Study_of_the_concordance_between_p16_immunohistochemistry_and_HPV_PCR_genotyping_for_the_viral_diagnosis_of_oropharyngeal_squamous_cell_carcinoma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1879-7296(15)00004-6 DB - PRIME DP - Unbound Medicine ER -